A Solid-Phase Assay for the Quantitation of Total Protein Eluted from Balafilcon, Lotrafilcon, and Etafilcon Contact Lenses

Glasier, Mary-Ann; Subbaraman, Lakshman N.; Senchyna, Michelle; Jones, Lyndon

doi:10.1080/02713680802291537

Cited by 7 publications

(1 citation statement)

References 39 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Таким образом, именно эти белки осуществляют основной вклад в двухфазную картину изменений общего уровня белка слезы. Хотя выяснение роли слёзных желез и продуцируемых ими белков в механизме развития ПЭР требует дальнейших исследований, три белка, а именно серотрансферрин, сывороточный альбумин и аннексин А1 можно считать маркерами этого осложнения, мониторинг содержания которых в слёзной жидкости не представляет особой сложности [16,17].…”

Section: результатыunclassified

Mechanisms of perioperative corneal abrasions: alterations in tear film proteome

et al. 2016

View full text Add to dashboard Cite

Perioperative corneal abrasion is an ophthalmic complication commonly found in patients underwent general anesthesia. In this study, correlations between development of corneal injury and proteomic changes in tear film during general anesthesia were examined using an animal (rabbit) model. Being started after 1-h anesthesia, the process of accumulation of pathological changes in the cornea unequivocally led clinically significant abrasions following 3-6 h of the narcosis. The corneal damage was associated with alterations in profiles of major proteins of the tear film. Analysis of the tear proteome pointed to depression of lachrymal glands function, and suggested serotransferrin, serum albumin and annexin A1 as potential tear markers of the complication. The tear film alterations included fast drop of total antioxidant activity and activity of superoxide dismutase, and decrease in interleukin-4 and increase in interleukin-6 content indicating development of oxidative and pro-inflammatory responses. These findings suggest antioxidant and anti-inflammatory therapy as prospective approach for prevention/treatment of perioperative corneal abrasions. The observed anesthesia-induced effects should be considered in any study of ocular surface diseases employing anesthetized animals.

show abstract

Section: результатыunclassified

Mechanisms of perioperative corneal abrasions: alterations in tear film proteome

et al. 2016

View full text Add to dashboard Cite

show abstract

A Review of Techniques to Measure Protein Sorption to Soft Contact Lenses

Hall

Forrest

Jones

2017

Eye &Amp; Contact Lens: Science &Amp; Clinical Practice

View full text Add to dashboard Cite

There are a wide variety of techniques to measure both the amount of protein and the biological activity of protein sorbed to soft contact lens materials. To measure the mass of protein sorbed to soft contact lenses (not just thin films) over short time periods, the method of choice should be I radiolabeling. This technique is sensitive enough to measure small amounts of deposited protein, provided steps are taken to limit and measure any interaction of the iodine tracer with the materials. To measure the protein activity over short time periods, the method of choice should be to measure the biological function of sorbed proteins. This may require new methods or adaptations of existing ones.

show abstract

Efficacy of an Extraction Solvent Used to Quantify Albumin Deposition on Hydrogel Contact Lens Materials

Subbaraman

Glasier²,

Sheardown³

et al. 2009

Eye &Amp; Contact Lens: Science &Amp; Clinical Practice

Self Cite

View full text Add to dashboard Cite

show abstract

A Solid-Phase Assay for the Quantitation of Total Protein Eluted from Balafilcon, Lotrafilcon, and Etafilcon Contact Lenses

Abstract: Both the AB on NC and CBQCA assays can be used to measure protein in extracts of lotrafilcon, balafilcon, and etafilcon lens materials.

Cited by 7 publications

References 39 publications

Mechanisms of perioperative corneal abrasions: alterations in tear film proteome

Mechanisms of perioperative corneal abrasions: alterations in tear film proteome

A Review of Techniques to Measure Protein Sorption to Soft Contact Lenses

Efficacy of an Extraction Solvent Used to Quantify Albumin Deposition on Hydrogel Contact Lens Materials

Contact Info

Product

Resources

About