A soft-template nanostructured peroxidase based on cytochrome c and sodium decyl sulfate and its electrochemical properties on hydroxyl fullerenes modified glassy carbon electrode

Yang, Xiu-yu; Zhang, Yushuai; Tian, Yang; Geng, Fang-Yong; Li, Di; Xiao, Bao-Lin; Hong, Jun; Moosavi-Movahedi, Ali Akbar; Ghourchian, Hedayatollah

doi:10.1007/s13738-015-0756-z

Cited by 7 publications

(5 citation statements)

References 52 publications

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“…The regression equations were I pc = 0.036 ν + 1.243 (R 2 = 0.9965) and I pa = −0.036 ν − 1.668 (R 2 = 0.995). In Figure 5 C, the absolute slopes value of log I vs. log ν were close to 1, so these indicate that the electrochemical behavior of rutin on the electrode surface is an adsorption-controlled process [ 16 ], which is the same as in other reports [ 51 , 52 ]. This phenomenon suggests that the determination of rutin should start from a low concentration, or the adsorbed rutin in the blank phosphate-buffered solution should be removed after detecting a high concentration of rutin.…”

Section: Resultssupporting

confidence: 81%

MWCNTs-CTAB and HFs-Lac Nanocomposite-Modified Glassy Carbon Electrode for Rutin Determination

Song

Xiang²,

Xiao³

et al. 2022

Biosensors

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Rutin is a flavonoid glycoside compound, which is mainly transported via the blood circulation system in the human body. The monitoring of the blood concentration of rutin is of great significance in many fields such as pharmacology and pharmacokinetics. In this work, a biosensor based on multi-walled carbon nanotubes (MWCNTs), cetyltrimethylammonium bromide (CTAB), hydroxyl fullerenes (HFs), and laccase (Lac) nanocomposite-modified glassy carbon electrodes was constructed. The modified materials were characterized with a transmission electron microscope (TEM), cyclic voltammograms (CV), and electrochemical impedance spectroscopy (EIS). CTAB is used to disperse MWCNTs and improve hydrophilicity and biocompatibility of MWCNTs, while the use of Lac can enhance the oxidation of catechol structure in rutin, thus significantly improving the sensitivity and selectivity of the modified electrode. Linear sweep voltammetry (LSV) studies showed that the determination linear ranges of rutin were 0.1 µmol L−1 to 2 µmol L−1 and 2 µmol L−1 to 11 µmol L−1, with the determination limits of 30 nmol L−1 and 95.5 nmol L−1, respectively. The proposed biosensor can be used to detect rutin tablets and serum samples with high recovery, which indicates a good accuracy of this method, and the results are consistent with those measured by the traditional ultra-high performance liquid chromatography (UHPLC) method. Hence, this biosensor has potential practical application value in rutin drug quality testing and clinical blood drug concentration monitoring.

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Section: Resultssupporting

confidence: 81%

MWCNTs-CTAB and HFs-Lac Nanocomposite-Modified Glassy Carbon Electrode for Rutin Determination

Song

Xiang²,

Xiao³

et al. 2022

Biosensors

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“…6). The detection limit based on 3s b (s b was the standard deviation of ten replicate measurements of fabricated biosensor in [Fe(CN) 6 3−/4− ] solution) was obtained to be 2.5 × 10 −15 M. These results shows that GO due to their unique properties such as having lots of carboxylic acid groups, increasing the surface area, good biocompatibility and electron mobility at room temperature exhibits high sensitivity. Also, some characteristics of ssDNA/GO/GCE biosensor were compared with the reported electrodes in Table 1.…”

Section: Investigation Of Sensitivity Of Modified Electrodementioning

confidence: 78%

“…From the slope of the I peak versus ν 1/2 at various scan 6 ] solution, the microscopic areas for GCE and GO/GCE were evaluated to be 0.0701 and 0.0910 cm 2 , respectively. Also, the current densities of modified and non-modified electrodes were calculated.…”

Section: Characterization Of Modified Electrodementioning

confidence: 99%

Application of graphene oxide nanosheets as probe oligonucleotide immobilization platform for DNA sensing

et al. 2016

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“…It could be seen that the linear range for measurement of GOD activity was from 4.3×10 -5 mM to 9.3×10 -3 mM (Fig.5A). The detection limit was 4.3×10 -5 mM (Fig.5B) [20][21][22]. …”

Section: E Linear Range and Detection Limitmentioning

confidence: 99%

A Sensitive and Reproducible Spectrophotometric Assay for Glucose Oxidase Activity and Its Application in Conformational Lock

Geng¹,

Li²,

Gao³

et al. 2017

Proceedings of the 2016 International Conference on Biological Engineering and Pharmacy (BEP 2016)

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contributed equally to this work.Abstract-Glucose-oxidase-horseradish peroxidaseguaiacol coupled system was applied for glucose oxidase assay with high sensitivity and prefect reproducibility. The optimum pH value was 5.8. The Michaelis-Menten constant (Km) and Vmax were determined to be 30.0±1.0mM and 2.49±0.08μM/s, respectively, for the assay system at 25º C. The relative standard deviation was 2.8% for 31 times measurements of GOD activity. The GOD assay could be applied in conformational lock study on GOD. Results of bio-thermodynamics suggested that the number of contact area of GOD is two, which is consistent with the computation results by using Ligplot and Pymol.

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A soft-template nanostructured peroxidase based on cytochrome c and sodium decyl sulfate and its electrochemical properties on hydroxyl fullerenes modified glassy carbon electrode

Cited by 7 publications

References 52 publications

MWCNTs-CTAB and HFs-Lac Nanocomposite-Modified Glassy Carbon Electrode for Rutin Determination

MWCNTs-CTAB and HFs-Lac Nanocomposite-Modified Glassy Carbon Electrode for Rutin Determination

Application of graphene oxide nanosheets as probe oligonucleotide immobilization platform for DNA sensing

A Sensitive and Reproducible Spectrophotometric Assay for Glucose Oxidase Activity and Its Application in Conformational Lock

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