Application of graphene oxide nanosheets as probe oligonucleotide immobilization platform for DNA sensing

Benvidi, Ali; Tezerjani, Marzieh Dehghan; Firouzabadi, Afsaneh Dehghani; Mazloum-Ardakani, Mohammad; Moshtaghioun, Seyed Mohammad

doi:10.1007/s13738-016-0931-x

Cited by 5 publications

(1 citation statement)

References 30 publications

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“…So, the electrochemical biosensors based on electrochemical techniques such as cyclic voltammetry (CV), differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy (EIS) have been developed for detection of low concentrations of DNA sequences. 14,15 Some great advantages of electrochemical biosensors compared to other types of biosensors are miniaturization, high sensitivity, low cost and fast detection. 16 The immobilization step of the DNA probe on the electrode surface is important in determining the overall performance of an electrochemical DNA biosensor.…”

Section: Introductionmentioning

confidence: 99%

A Highly Selective DNA Sensor Based on Graphene Oxide-Silk Fibroin Composite and AuNPs as a Probe Oligonucleotide Immobilization Platform

Benvidi¹,

Abbasi²,

Tezerjani³

et al. 2018

ACSi

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In this study, a simple and novel electrochemical biosensor based on a glassy carbon electrode (GCE) modified with a composite of graphene oxide (GO) - silk fibroin nanofibers (SF) and gold nanoparticles (MCH/ssDNA/AuNPs/SF/GO/GCE) was developed for detection of DNA sequences. The fabrication processes of electrochemical biosensor were characterized by scanning electron microscopy (SEM), FT-IR and electrochemical methods. Some experimental conditions such as immobilization time of probe DNA and MCH incubation time, time and temperature of hybridization were optimized. The designed biosensor revealed a wide linear range of 1.0 × 10-16 - 1.0 × 10-8 mol L-1 and a low detection limit (3.3 × 10-17 mol L-1) for detection of BRCA1 5382 mutation by EIS technique. The designed biosensor revealed high selectivity for discrimination of the complementary (P1C) sequences from various non-complementary sequences of (P1nC1, P1nC2 and P1nC3). Also, the biosensor revealed a high reproducibility (RSD of 7.5% (n=4)) and high stability (92% of its initial response after 8 days). So, the fabricated biosensor has a suitable potential to be applied for detection of breast cancer sequences in the initial stages of the cancer.

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Section: Introductionmentioning

confidence: 99%