A sodium-and potassium-stimulated adenosine triphosphatase from cardiac tissues—II

Schwartz, Arnold; Laseter, Arline H.

doi:10.1016/0006-2952(64)90149-2

Cited by 44 publications

(4 citation statements)

References 19 publications

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“…3). This effect seems comparable with the effects of media of high ionic strength on microsomal ATPases (Schwartz & Laseter, 1964;Bray, 1973;Izutsu et al, 1974). Sucrose at 250mM concentration had no effect on activity.…”

Section: Substrate Specificitysupporting

confidence: 72%

Properties of mammalian nuclear-envelope nucleoside triphosphatase

Agutter¹,

Cockrill²,

Lavine³

et al. 1979

Biochemical Journal

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The nucleoside triphosphatase activities of the nuclear envelopes from rat liver, pig liver and simian-virus-40-transformed mouse-embryo 3T3 cells were shown to exhibit similar parperties. All three preparations hydrolyse ATP, 2'-dATP, 3'-dATP, GTP, CTP and UTP in the presence of Mg2+, Ca2+, Mn2+ and Co2+ with a pH optimum of 8.0, are sensitive to inhibition by mercurials, arsenicals, quercetin, proflavin and adenosine 5'-[gamma-thio]triphosphate and are partially inactivated by exposure to high ionic strength. The kinetic behaviour is similar for all substrates irrespective of the source of material. The typical Eadie-Hofstee plot, which is concave upwards at pH 8.0 when the ionic strength is 20mM, becomes linear when the pH is increased to 8.5 or the ionic strength to 160mM. The overall evidence, particularly the labelling of only one polypeptide by [gamma-32P]ATP, suggests that under the conditions of preparation and assay used only one class of nucleoside triphosphatase active sites is detectable in nuclear envelopes. The importance of these results for an understanding of the role of the enzyme in vivo is discussed.

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Section: Substrate Specificitysupporting

confidence: 72%

Properties of mammalian nuclear-envelope nucleoside triphosphatase

Agutter¹,

Cockrill²,

Lavine³

et al. 1979

Biochemical Journal

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“…In plants, K takes part in osmoregulation [1,2], influences enzyme activities [3,4] and is involved in energy metabolism [5]. In animals, K plays a crucial role in nerve functioning [6,7], also influences enzyme activities [8][9][10][11][12] and affects the acid-base balance [13]. To fulfill the needs of fish in freshwater aquaculture, commercial diets for the African catfish (Clarias gariepinus) contain about 0.94-0.97% of K [14].…”

Section: Introductionmentioning

confidence: 99%

Effects of Dissolved Potassium on Growth Performance, Body Composition, and Welfare of Juvenile African Catfish (Clarias gariepinus)

Wenzel

Strauch

Eding

et al. 2021

Fishes

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Optimal crop production in aquaponics is influenced by water pH and potassium concentrations. The addition of potassium hydroxide (KOH) into the recirculating aquaculture system (RAS) may benefit aquaponics by increasing the water pH for better biofilter activity and supplementing K for better plant growth and quality. We investigated the growth, feed conversion, body composition and welfare indicators of juvenile African catfish (Clarias gariepinus) treated with four concentrations of K (K0 = 2, K200 = 218, K400 = 418, and K600 = 671 mg L−1). While growth, feed conversion and final body composition were unaffected, the feeding time and individual resting significantly increased with increasing K+. The swimming activity and agonistic behavior were reduced significantly under increased concentrations of K+. Leftover feed and the highest number of skin lesions were observed under K600. We suggest that K+ concentrations between 200 and 400 mg L−1 can improve the welfare status of juvenile African catfish. This enables the application of KOH in RAS to supply alkalinity to achieve optimum nitrification at minimum water exchange and improve the nutritional profile of the process water with benefits for the welfare status of African catfish and aquaponics plant production and quality.

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“…(C4H10NSC1) C, , N, S. Biological studies were carried out using adipose tissue from nonfasted, white male Harlan Wistar rats according to methods previously published. 21 All drug concentrations were added in 0.1 ml of aqueous solution. ß-Mercaptoethylamine hydrochloride was purchased from Aldrich Chemical Co., Milwaukee, Wis., and used in these studies without further purification.…”

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confidence: 99%

“…In terms of compounds with the common di-n-butylaminomethyl side chain, the Rane data show that a single Cl substituent on each ring (4) resulted in optimum activity (except for the highly effective CF3 group which will be reported in a following paper). Increasing the number of Cl substituents to three (21) or four (23) resulted in a marked decrease in activity. Replacement of one Cl with Br (15), or both with Br (18), resulted in a somewhat decreased activity.…”

mentioning

confidence: 99%