The reuse of effluent waters and sediments from African catfish (Clarias gariepinus) recirculation aquaculture systems requires a deeper understanding of the nutrient and energy flows and material pathways. Three semi-commercial systems, differing in stocking density, were sampled for nutritive and pollutant elements of the input-(tap water, feed) and output pathways (fillet, carcass, process water, sediments) by ICP-OES/MS and calorimetry. Highly water-soluble elements, e.g., potassium, accumulated in the water, whereas iron, copper, chromium and uranium where found in the solids. Feed derived phosphorous was accounted for, 58.3-64.2% inside the fish, 9.7-19.3% in sediments, and small amounts 9.6-15.5% in the process waters. A total of 7.1-9.9% of the feed accumulated as dry matter in the sediments, comprising 5.5-8.7% total organic carbon and 3.7-5.2% nitrogen. A total of 44.5-47.1% of the feed energy was found in the fish and 5.7-7.7% in the sediments. For reuse of water and nutrients in hydroponics, the macro-nutrients potassium, nitrate, phosphorus and the micro-nutrient iron were deficient when compared with generalized recommendations for plant nutrition. Low energy contents and C/N-ratio restrict the solely use of African catfish solids for biogas production or vermiculture. Using the outputs both for biogas supplement and general fertilizer in aquaponics farming (s.l.) (combined with additional nutrients) appears possible.

Aquaponics production of spearmint (Mentha spicata) was evaluated under commercial grow-out conditions of African catfish (Clarias gariepinus) in Northern Germany (Mecklenburg-Western Pomerania). Fish batch production under different stocking densities in an extensive aquacultural unit (EAU) and an intensive aquacultural unit (IAU) was connected to conventional plant cultivation on ebb-and-flood planting tables and compared to a liquid fertilizer control. The best growth parameters of M. spicata were found under the intensive stocking density of C. gariepinus (IAU), resulting in a plant leaf area of 10.9 ± 2.5 cm2, leaf length of 8.6 ± 1.6 cm, and a cut fresh biomass from aboveground of 31.8 ± 13.8 g/plant, compared to the EAU (5.6 ± 2.1 cm2; 5.4 ± 1.4 cm; 17.4 ± 4.7 g/plant) and the control (5.7 ± 2.2 cm2; 5.5 ± 1.4 cm; 11.2 ± 5.3 g/plant). The fresh biomass of the whole plants was not significantly different between the EAU (165.5 ± 71.7 g/plant) and the IAU (190.7 ± 105.6 g/plant), though the latter gained more weight. The initial fish number ratio between the EAU and the IAU of 1/4 increased the M. spicata leaf area by twofold in the IAU. Our results demonstrate that aquaponics (s.l.) production of M. spicata is possible under the direct use of effluent waters from intensive African catfish cultivation without the addition of any liquid fertilizer.

Optimal crop production in aquaponics is influenced by water pH and potassium concentrations. The addition of potassium hydroxide (KOH) into the recirculating aquaculture system (RAS) may benefit aquaponics by increasing the water pH for better biofilter activity and supplementing K for better plant growth and quality. We investigated the growth, feed conversion, body composition and welfare indicators of juvenile African catfish (Clarias gariepinus) treated with four concentrations of K (K0 = 2, K200 = 218, K400 = 418, and K600 = 671 mg L−1). While growth, feed conversion and final body composition were unaffected, the feeding time and individual resting significantly increased with increasing K+. The swimming activity and agonistic behavior were reduced significantly under increased concentrations of K+. Leftover feed and the highest number of skin lesions were observed under K600. We suggest that K+ concentrations between 200 and 400 mg L−1 can improve the welfare status of juvenile African catfish. This enables the application of KOH in RAS to supply alkalinity to achieve optimum nitrification at minimum water exchange and improve the nutritional profile of the process water with benefits for the welfare status of African catfish and aquaponics plant production and quality.

Recycling of phosphorus (P) from feed input in aquaculture systems gains increasing importance, especially relating to sustainable agriculture and food production. In order to find possible areas of application of African catfish solid waste, the purpose of this study was to characterize the elemental and organic matter composition and P speciation in the aquaculture fish waste. Pyrolysis-field ionization mass spectrometry (Py-FIMS) was used to investigate the composition of organic matter and P K-edge X-ray absorption near edge structure (XANES) spectroscopy to describe the occurring P-containing compounds in African catfish solid waste from an intensive recirculation aquaculture system (RAS). The solid fish waste was mainly composed of sterols, free fatty acids and alkylaromatics, as it is common for digestive systems of animals. Ingredients such as the phytosterol beta-sitosterin confirm plant-based feed ingredients and some recalcitrance against digestion in the African catfish gut. The P in the solid fish waste was exclusively bound as calcium-phosphates. These calcium-phosphate minerals as major constituents of African catfish waste may have beneficial effects when applied to soils, suggesting the use of this waste as possible soil amendment in the future.

The effects of semi-intensive (100 kg m−3), intensive (200 kg m−3), and super-intensive (400 kg m−3) stocking densities on the growth and welfare of African catfish (Clarias gariepinus) were investigated under commercial production conditions. Plasma cortisol, glucose, and selected transcripts following a stress challenge, lactate, as well as skin lesions, were analyzed at regular intervals (from 12 g juveniles to 1.5–2.0 kg). The fish grew well, but after 23 weeks, the semi-intensively stocked fish had a mean final weight of 1,830.5 g, significantly higher than the super-intensively stocked fish with 1,615.4 g, and considerably higher than the intensively stocked fish with 1,664.8 g (p > 0.05). Cortisol and glucose responses significantly differed between stressed and unstressed fish, but not between treatment groups. An unforeseen external stressor (nearby demolition noise) caused stress responses among all treatment groups, but was similarly coped with. Mortality ranged between 3.8–9.2%. In the juveniles, skin lesions were reduced under intensive or super-intensive densities, with the least under semi-intensive densities in outgrown fish. Expression profiles of 22 genes were compared in the spleen at semi-intensive and super-intensive densities. The transcript concentrations of most genes remained unchanged, except for slc39a8 and mtf1, which were significantly downregulated in stressed catfish under semi-intensive conditions. We demonstrated that African catfish growth performance and welfare depend on age and stocking density, also reacting to demolition noise. This supports farm management to optimize stocking densities during the grow-out of African catfish in RAS and suggests avoiding external stress.