A smart injectable composite hydrogel with magnetic navigation and controlled glutathione release for promoting in situ chondrocyte array and self-healing in damaged cartilage tissue

Abstract

Injectable cell-based hydrogels allow surgical operation in a minimally invasive way for articular cartilage lesions but the chondrocytes in the injectable hydrogels are difficultly arrayed and fixed at the site...

Advanced injectable hydrogels for cartilage tissue engineering

“…The chondrocytes embedded in the hydrogel proliferated and differentiated at the site of cartilage damage through magnetic interaction of internal iron nanoparticles and adhesion of CD44 receptors on HA chain. The rabbit cartilage defect model produced uniform and smooth, regenerated cartilage 8 weeks after hydrogel implantation, and the columnar arrangement of chondrocytes in the deep tissue was similar to that of normal chondrocytes ( Chiang et al, 2021 ).…”

“…The chondrocytes proliferated well in vitro , promoting cartilage defect repair in rat models in vivo . Chiang et al (2021) prepared an injectable HA-PAA hydrogel with magnetic navigation and glutathione release. The chondrocytes embedded in the hydrogel proliferated and differentiated at the site of cartilage damage through magnetic interaction of internal iron nanoparticles and adhesion of CD44 receptors on HA chain.…”

Designing functional hyaluronic acid-based hydrogels for cartilage tissue engineering

“… Hydrogels Components Physicochemical properties Biofunctions Ref. HA-ADH/d-AHA HA, adipic dihydrazide, dialdehyde HA Shear-responsive; boundary lubricated; anti-inflammatory; injectable; self-healing; controlled-release of bioactive molecules Reducing friction; alleviating the progression of OA; inhibiting inflammation; protecting chondrocytes [ 141 , 142 ] HA-ADH/dextran HA, adipic dihydrazide, dialdehyde dextran ROS depletion, sustainable drug release; viscosupplementation Inhibiting inflammation; alleviating the progression of OA; protecting cartilage [ 143 ] HA-ADH/pectin HA, adipic dihydrazide, oligopeptide-grafted dialdehyde pectin Short gel times; controllable mechanical properties and degradation capacity Providing a suitable microenvironment for the phenotype of chondrocytes and chondrogenesis [ 144 ] HA-Fur/CS-Mal/ACS/ADH Methylfuran-modified HA, maleimide-modified chondroitin sulfate, adipic dihydrazide, dialdehyde chondroitin sulfate Double-network; controllable viscoelasticity and stress relaxation; good processability; self-healing ability; high swelling capacity and stability Negligible inflammatory response [ 145 ] HA-Ad/ β -CD-Ac HA, adamantane, monoacrylated β -cyclodextrin Good self-healing properties, compressibility; sustained release of TGF- β 1 Rapidly integrating with the defect tissue; promoting the chondrogenesis of MSCs and cartilage regeneration [ 146 ] HA-Ad/ β -CD-SH/HA-Mal HA, adamantane, thiolated β -cyclodextrin, maleimide Tunable viscoelasticity Maintaining 3D spreading and intracellular interactions of encapsulated MSCs [ 147 ] HA- β -CD/PAA-Fer HA, β -cyclodextrin, ferrocene, polyacrylic acid Magnetic navigation; controlled release of glutathione Promoting the columnar arrangement of chondrocytes and the repair of cartilage damage [ 148 ] HA-CB [ 6 ]/HA-PA HA, cucurbit [ …”

“…first developed a smart injectable composite hydrogel using β -CD-modified HA (HA- β -CD) and ferrocene-modified polyacrylic acid through host–guest interactions to encapsulate glutathione (GSH)-loaded magnetic microcapsules. The hydrogel exhibited magnetic navigation and controlled release of GSH, which can effectively promote the columnar arrangement of chondrocytes and the repair of cartilage damage [ 148 ]. Park et al.…”

Synergistic Effect of Static Magnetic Fields and 3D-Printed Iron-Oxide-Nanoparticle-Containing Calcium Silicate/Poly-ε-Caprolactone Scaffolds for Bone Tissue Engineering

“…Extrinsic mechanical stimulations, such as vibration, ultrasound, and electrical stimulation, have been shown to promote bone regeneration and enhance fracture healing [ 17 , 18 , 19 , 20 ]. Because of the development of magnetic nanoparticles (MNPs), magnetic stimulation has been reported to have the best potential for effective bone regenerative capabilities [ 21 , 22 , 23 ]. MNPs have been introduced into various matrices and platforms in order to induce stimuli-responsive regenerative properties for osteogenesis [ 24 , 25 , 26 ].…”