A Smart Fluorescent Probe for NO Detection and Application in Myocardial Fibrosis Imaging

Abstract

On the basis of the pyridazinone scaffold and photoinduced electron transfer (PET) mechanism, we designed a smart nitric oxide (NO) probe, PYSNO, with high sensitivity and selectivity. PYSNO exhibited a rapid response to both exogenous and endogenous NO. This probe can also be used in tracking and investigating NO generation in animal tissue. In the myocardial fibrosis model for mice, PYSNO exhibited a powerful imaging property in vivo as a result of unravelling the progressive relationship between the generation of myocardial NO and the occurrence of myocardial fibrosis.

Small-molecule fluorescence-based probes for interrogating major organ diseases

“…Xu et al designed a small molecule-based fluorescent probe, PYSNO, that permits the selective recognition of NO. 66 They used pyridazinone as a fluorescence unit, o-phenylenediamine as the recognition group for NO, and an alkyl morpholine as the lysosome-targeting moiety (Fig. 44).…”

“…Chemical structure of small molecule-based fluorescent probe PYSNO and the reaction-based conversion that makes it effective as a probe for NO as judged from studies carried out in an isoproterenol (ISO)-induced MF mouse model. Reproduced with permission from ref 66…”

A Highly Stable Two-Photon Ratiometric Fluorescence Probe for Real-Time Biosensing and Imaging of Nitric Oxide in Brain Tissues and Larval Zebrafish

“…and the detection limit was estimated to 105.0 ± 3.1 nM (S/N = 3, n = 20). In comparison with the fluorescent NO probes in the previous literatures, [36][37][38][39][40][41][42] our developed RBD@AuNCs probe showed a wider detection linear range. It should be noted that the absorbance intensity of individual RBD increased apparently around 556 nm with the increasing concentrations of NO, accompanied with fluorescence enhancement around 580 nm (Supporting information Figure S12).…”

“…Thus, the increase of TPF emission at ~580 nm can be attributed to the production of Rhodamine B, which was further confirmed by MS (Supporting information Figure S13). In addition, the response time of developed RBD@AuNCs towards NO (100 <-was evaluated to ~55 s (Figure 2c), which was faster than most of reported fluorescence methods for NO sensing, [36][37][38][39][40][41][42] as summarized in Supporting information Table S2. Moreover, the signals variations for RBD@AuNCs was less than 3.7% at pH 6.5-8.0, and little signal decrease (< 4.2%) was found for RBD@AuNCs when explored to a Xe lamp (90 W) for 150 min (Supporting information Figure S14), indicating the high stability of the designed RBD@AuNCs nanoprobe.…”

Fluoride ion‐induced gas sensor based on the dipyrromethene boron difluoride derivative: A theoretical investigation

“…Moreover, the fluorescence response signal units are easily available, [10] and the fluorescence properties can be modulated by developing novel fluorophores and modifying electronic groups. Therefore, fluorescence gas sensors based on various dyes and fluorophores have been applied to detect the gases, including CO 2, [11,12] CO, [13] NO, [14] NH 3, [15] H 2 S, [16] and SO 2. [17] Compared with conventional dyes or fluorophores, such as fluorescein, [18] Rhodamine, [19] and coumarin [20] which acts as a response signal unit, Dipyrromethene boron difluoride (BODIPY) dyes are an important class of fluorescent dyes that possess many advantages, for example, high fluorescence quantum yields, larger molar extinction coefficient, good photostability, and pH insensitivity.…”