A Size-Matching Heterotopic Aortic Valve Implantation Model in the Rat

Oei, Frans B S; Welters, Marij J.P.; Bonthuis, Fred; Vaessen, L. M. B.; Marquet, R. L.; Zondervan, Pieter E.; Weimar, Willem; Bogers, Ad J.J.C.

doi:10.1006/jsre.1999.5763

Cited by 10 publications

(6 citation statements)

References 16 publications

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“…However, detailed studies that assess the immunologic response to cellular and acellular AVA and its eff ect on the valves structural integrity are lacking. Therefore, in the present study we used a recently developed two-step detergent-enzymatic extraction method [6] and a rat aortic transplantation model [6,7] to investigate the relationship between the cellular immune response and structural damage.…”

Section: Discussionmentioning

confidence: 99%

“…All operations were performed using a modifi cation of a technique [6] fi rst described by Oei et al [7] Histolog y Vector). Finally peroxidase-conjungated streptavidin (1:400; Dako) was added and detected by diaminobenzidine tetrahydrochloride for 8 minutes at room temperature.…”

Section: Surgical Techniquementioning

confidence: 99%

“…Recently we have developed a two-step detergent-enzymatic extraction method to produce completely decellularized rat aortic valve conduits with minimal damage to the ECM [6]. In a rat aortic valve transplantation model [7] transplantation of decellularised AVA between MHC-mismatched rats, valves were still morphologically intact after 3 weeks, whereas transplanted cellular allografts became deformed and swollen with major morphological changes to the ECM, especially collagen [6].…”

Section: Introductionmentioning

confidence: 99%

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Decellularization of rat aortic valve allografts reduces leaflet destruction and extracellular matrix remodeling

Grauss

Hazekamp

Vliet

et al. 2003

The Journal of Thoracic and Cardiovascular Surgery

122

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Section: Discussionmentioning

confidence: 99%

Section: Surgical Techniquementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Decellularization of rat aortic valve allografts reduces leaflet destruction and extracellular matrix remodeling

Grauss

Hazekamp

Vliet

et al. 2003

The Journal of Thoracic and Cardiovascular Surgery

122

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“…The aortic valve was dissected from the heart under a microscope as a U-shaped aortic conduit as described by others. 22,23 Briefly, a myocardial cuff of *1-2 mm and part of the anterior mitral valve leaflet were preserved, whereas the brachiocephalic artery, left common carotid artery, and left subclavian artery were ligated with 6-0 sutures (Ethicon) at their origin. The descending aorta was divided at the level of the aortic valve.…”

Section: Rat Aortic Valve Procurementmentioning

confidence: 99%

Characterization of Laminins in Healthy Human Aortic Valves and a Modified Decellularized Rat Scaffold

Granath

Noren

Björck

et al. 2020

BioResearch Open Access

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Aortic valve stenosis is one of the most common cardiovascular diseases in western countries and can only be treated by replacement with a prosthetic valve. Tissue engineering is an emerging and promising treatment option, but in-depth knowledge about the microstructure of native heart valves is lacking, making the development of tissue-engineered heart valves challenging. Specifically, the basement membrane (BM) of heart valves remains incompletely characterized, and decellularization protocols that preserve BM components are necessary to advance the field. This study aims to characterize laminin isoforms expressed in healthy human aortic valves and establish a small animal decellularized aortic valve scaffold for future studies of the BM in tissue engineering. Laminin isoforms were assessed by immunohistochemistry with antibodies specific for individual α, β, and γ chains. The results indicated that LN-411, LN-421, LN-511, and LN-521 are expressed in human aortic valves ( n = 3), forming a continuous monolayer in the endothelial BM, whereas sparsely found in the interstitium. Similar results were seen in rat aortic valves ( n = 3). Retention of laminin and other BM components, concomitantly with effective removal of cells and residual DNA, was achieved through 3 h exposure to 1% sodium dodecyl sulfate and 30 min exposure to 1% Triton X-100, followed by nuclease processing in rat aortic valves ( n = 3). Our results provide crucial data on the microenvironment of valvular cells relevant for research in both tissue engineering and heart valve biology. We also describe a decellularized rat aortic valve scaffold useful for mechanistic studies on the role of the BM in heart valve regeneration.

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“…They speculate that this is due mainly to a lack of leaflet mobility in a nonfunctional implantation model. 1 To overcome this difficulty Légaré and Ross suggest the use of a functional rat implantation model in which the creation of native aortic valve insufficiency results in a significant diastolic flow reversal in the abdominal aorta, allowing leaflets to move and function. 2 Although we were aware of the existence of this model, we decided not to use it for our study.…”

Section: Reply To the Editormentioning

confidence: 99%