A single-step, high throughput, and highly reproducible method for measuring IgM quantity and avidity directly from fish serum via biolayer interferometry (BLI)

Li, Angus; Harris, Richard J.; Fry, Bryan G.; Barnes, Andrew C.

doi:10.1016/j.fsi.2021.10.003

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Cited by 3 publications

References 37 publications

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Protein-Protein Binding Kinetics by Biolayer Interferometry

Santos-López,

Gómez,

Fernández

et al. 2024

Advances in Experimental Medicine and Biology

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Protein-Protein Binding Kinetics by Biolayer Interferometry

Santos-López,

Gómez,

Fernández

et al. 2024

Advances in Experimental Medicine and Biology

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Biolayer interferometry is insufficiently sensitive for direct measurement of IgM quantity and avidity in Atlantic salmon (Salmo salar) serum

English

Barnes

2023

Fish & Shellfish Immunology

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PDZ2-conjugated-PLGA nanoparticles are tiny heroes in the battle against SARS-CoV-2

Giacon,

Lo Cascio,

Pennacchietti

et al. 2024

Sci Rep

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The COVID-19 pandemic caused by SARS-CoV-2 has highlighted the urgent need for innovative antiviral strategies to fight viral infections. Although a substantial part of the overall effort has been directed at the Spike protein to create an effective global vaccination strategy, other proteins have also been examined and identified as possible therapeutic targets. Among them, although initially underestimated, there is the SARS-CoV-2 E-protein, which turned out to be a key factor in viral pathogenesis due to its role in virus budding, assembly and spreading. The C-terminus of E-protein contains a PDZ-binding motif (PBM) that plays a key role in SARS-CoV-2 virulence as it is recognized and bound by the PDZ2 domain of the human tight junction protein ZO-1. The binding between the PDZ2 domain of ZO-1 and the C-terminal portion of SARS-CoV-2 E-protein has been extensively characterized. Our results prompted us to develop a possible adjuvant therapeutic strategy aimed at slowing down or inhibiting virus-mediated pathogenesis. Such innovation consists in the design and synthesis of externally PDZ2-ZO1 functionalized PLGA-based nanoparticles to be used as intracellular decoy. Contrary to conventional strategies, this innovative approach aims to capitalize on the E protein-PDZ2 interaction to prevent virus assembly and replication. In fact, the conjugation of the PDZ2 domain to polymeric nanoparticles increases the affinity toward the E protein effectively creating a “molecular sponge” able to sequester E proteins within the intracellular environment of infected cells. Our in vitro studies on selected cellular models, show that these nanodevices significantly reduce SARS-CoV-2-mediated virulence, emphasizing the importance of exploiting viral-host interactions for therapeutic benefit.

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A single-step, high throughput, and highly reproducible method for measuring IgM quantity and avidity directly from fish serum via biolayer interferometry (BLI)

Cited by 3 publications

References 37 publications

Protein-Protein Binding Kinetics by Biolayer Interferometry

Protein-Protein Binding Kinetics by Biolayer Interferometry

Biolayer interferometry is insufficiently sensitive for direct measurement of IgM quantity and avidity in Atlantic salmon (Salmo salar) serum

PDZ2-conjugated-PLGA nanoparticles are tiny heroes in the battle against SARS-CoV-2

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