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This study investigated the inter-day and intra-device reliability, and criterion validity of six devices for measuring barbell velocity in the free-weight back squat and power clean. In total, 10 competitive weightlifters completed an initial one repetition maximum (1RM) assessment followed by three load-velocity profiles (40–100% 1RM) in both exercises on four separate occasions. Mean and peak velocity was measured simultaneously on each device and compared to 3D motion capture for all repetitions. Reliability was assessed via coefficient of variation (CV) and typical error (TE). Least products regression (LPR) (R2) and limits of agreement (LOA) assessed the validity of the devices. The Gymaware was the most reliable for both exercises (CV < 10%; TE < 0.11 m·s−1, except 100% 1RM (mean velocity) and 90‒100% 1RM (peak velocity)), with MyLift and PUSH following a similar trend. Poorer reliability was observed for Beast Sensor and Bar Sensei (CV = 5.1–119.9%; TE = 0.08–0.48 m·s−1). The Gymaware was the most valid device, with small systematic bias and no proportional or fixed bias evident across both exercises (R2 > 0.42–0.99 LOA = −0.03–0.03 m·s−1). Comparable validity data was observed for MyLift in the back squat. Both PUSH devices produced some fixed and proportional bias, with Beast Sensor and Bar Sensei being the least valid devices across both exercises (R2 > 0.00–0.96, LOA = −0.36–0.46 m·s−1). Linear position transducers and smartphone applications could be used to obtain velocity-based data, with inertial measurement units demonstrating poorer reliability and validity.

dStreptococcus iniae causes severe septicemia and meningitis in farmed fish and is also occasionally zoonotic. Vaccination against S. iniae is problematic, with frequent breakdown of protection in vaccinated fish. The major protective antigens in S. iniae are the polysaccharides of the capsule, which are essential for virulence. Capsular biosynthesis is driven and regulated by a 21-kb operon comprising up to 20 genes. In a long-term study, we have sequenced the capsular operon of strains that have been used in autogenous vaccines across Australia and compared it with the capsular operon sequences of strains subsequently isolated from infected vaccinated fish. Intriguingly, strains isolated from vaccinated fish that subsequently become infected have coding mutations that are confined to a limited number of genes in the cps operon, with the remainder of the genes in the operon remaining stable. Mutations in strains in diseased vaccinated fish occur in key genes in the capsular operon that are associated with polysaccharide configuration (cpsG) and with regulation of biosynthesis (cpsD and cpsE). This, along with high ratios of nonsynonymous to synonymous mutations within the cps genes, suggests that immune response directed predominantly against capsular polysaccharide may be driving evolution in a very specific set of genes in the operon. From these data, it may be possible to design a simple polyvalent vaccine with a greater operational life span than the current monovalent killed bacterins.

This study aimed to identify performance indicators that discriminate winning and losing in elite men's and women's Rugby Union during the latest World Cup competitions. The knockout stages of the men's 2015 Rugby World Cup (n = 8) and women's 2014 Rugby World Cup (n = 8) were compared. Findings suggest that in the men's game, winning teams kicked a greater percentage of possession in the opposition 22-50m with a view to gaining territory and pressuring the opposition (winners = 16%, losers = 7%). In the women's game successful teams adopted a more possession driven attacking approach in this area of the pitch. Successful women's teams appear more willing to attack with ball in hand following a kick receipt and adopt a more expansive game through attacking with wider carries in the outside channels. The percentage of lineouts won on the opposition ball was found to be an important performance indicator that discriminates winners and losers, regardless of sex (winners = 18%, losers = 11%). Findings suggest successful men's and women's teams adopt different tactical approaches to knockout competitions, this knowledge should be used as a basis for coaches to develop tactical approaches and training methods which are sex dependent.

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Background: Binding of serum components by surface M-related proteins, encoded by the emm genes, in streptococci constitutes a major virulence factor in this important group of organisms. The present study demonstrates fibrinogen binding by S. iniae, a Lancefield non-typeable pathogen causing devastating fish losses in the aquaculture industry and an opportunistic pathogen of humans, and identifies the proteins involved and their encoding genes.

Photobacterium damselae subsp. piscicida (formerly Pasteurella pisckida) is the causative agent of pasteurellosis or pseudotuberculosis in warm water marine fish. Enzymes which neutralize reactive oxygen species, produced during aerobic metabolism or during respiratory burst in fish macrophages, are important virulence factors in many pathogens. This study characterizes a periplasmic superoxide dismutase (SOD) and a cytoplasmic catalase in P. damselae. Purification and partial amino-terminal sequencing confirmed the SOD to be iron-cofactored, with a high degree of homology to other bacterial FeSODs. The SOD was common to all strains analysed in terms of type, location and activity, whilst the catalase varied in activity between strains. The catalase was constitutively expressed, but the SOD appeared to be repressed under low oxygen conditions. In spite of the presence of a periplasmic SOD, P. damselae was susceptible to killing by exogenous superoxide anion generated in a cellfree system. Addition of exogenous SOD to this system did not abolish the bactericidal eft: ect; however, addition of catalase was protective. These results suggest that lack of periplasmic catalase may be implicated in susceptiblity t o killing by reactive oxygen species.