A single robust stability-indicating RP-HPLC analytical tool for apigenin quantification in bulk powder and in nanoliposomes: a novel approach

Shetti, Priya; Jalalpure, Sunil

doi:10.1186/s43094-021-00268-6

Cited by 6 publications

(3 citation statements)

References 22 publications

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“…The particle size of the coated nanoliposomes could be affected by the material type and preparation methods, such as ultrasonic dispersion and thin-film evaporation [ 43 , 44 ]. The PDI values give an indication of a particle size distribution in the emulsion, and the lower value showed higher stability [ 45 ]. The PDI values of empty nanoliposomes and different concentrations of RO nanoliposomes were between 0.266–0.283, and the different coated concentrations of RO did not significantly affect the PDI values of the nanoliposomes.…”

Section: Resultsmentioning

confidence: 99%

Physicochemical and Antioxidant Properties of Nanoliposomes Loaded with Rosemary Oleoresin and Their Oxidative Stability Application in Dried Oysters

et al. 2022

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Lipid and protein oxidation is a main problem related to the preservation of dried aquatic products. Rosemary oleoresin is widely used as an antioxidant, but its application is limited due to its instability and easy degradation. Nanoliposome encapsulation is a promising and rapidly emerging technology in which antioxidants are incorporated into the liposomes to provide the food high quality, safety and long shelf life. The objectives of this study were to prepare nanoliposome coatings of rosemary oleoresin to enhance the antioxidant stability, and to evaluate their potential application in inhibiting protein and lipid oxidation in dried oysters during storage. The nanoliposomes encapsulating rosemary oleoresin were applied with a thin-film evaporation method, and the optimal amount of encapsulated rosemary oleoresin was chosen based on changes in the dynamic light scattering, Zeta potential, and encapsulation efficiency of the nanoliposomes. The Fourier transform-infrared spectroscopy of rosemary oleoresin nanoliposomes showed no new characteristic peaks formed after rosemary oleoresin encapsulation, and the particle size of rosemary oleoresin nanoliposomes was 100–200 nm in transmission electron microscopy. The differential scanning calorimetry indicated that the nanoliposomes coated with rosemary oleoresin had better thermal stability. Rosemary oleoresin nanoliposomes presented good antioxidant stability, and still maintained 48% DPPH radical-scavenging activity and 45% ABTS radical-scavenging activity after 28 d of storage, which was 3.7 times and 2.8 times higher than that of empty nanoliposomes, respectively. Compared with the control, the dried oysters coated with rosemary oleoresin nanoliposomes showed significantly lower values of carbonyl, sulfhydryl content, thiobarbituric acid reactive substances, Peroxide value, and 4-Hydroxynonenal contents during 28 d of storage. The results provide a theoretical basis for developing an efficient and long-term antioxidant approach.

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Section: Resultsmentioning

confidence: 99%

Physicochemical and Antioxidant Properties of Nanoliposomes Loaded with Rosemary Oleoresin and Their Oxidative Stability Application in Dried Oysters

et al. 2022

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“…LOD and LOQ were assessed as per the ICH Guidelines Q2(R1) (21). Both parameters limit the amount of analyte that may be evaluated (23). The ratio of the detected amount to added amount was calculated to assess the average percentage of recoveries (18).…”

Section: Discussionmentioning

confidence: 99%

High-performance liquid chromatography method development and validation for the quantification of mangiferin in Coffea arabica leaves

Hallur¹,

Shaikh²,

Magare³

et al. 2023

Biomedicine

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Introduction and Aim: Mangiferin is accompanied by the therapeutic potential for various human ailments. The present study is aimed to develop a validated HPLC method for the quantification of mangiferin in coffee (Coffea arabica) leaves. Materials and Methods: The high-performance liquid chromatography (HPLC) was carried out on Agilent Technologies 1260 LC Infinity Series liquid chromatography system (Agilent, USA) using an isocratic mobile phase of a mixture of methanol and 1% orthophosphoric acid v/v (70:30) at a flow rate 1mL min-1 for 14 min with the controlled temperature at 25°C. The mangiferin content was detected by a UV diode array detector at 258 nm. Results: The linearity of the HPLC system was established in the range of 10-100 ppm mL-1 with a regression coefficient (R2) of 0.99670. The accuracy of the method was assessed by a study performed using 3 different levels, with a 99% average recovery. The limit of detection(LOD) and limit of quantification (LOQ) were 1.3 and 3.9 ppm mL-1. Intraday and interday precision analysis showed the relative standard deviation 0.012342 separately. The content of mangiferin in the coffee leaves was 94 mg/gram (w/w). Conclusion: All necessary factors, such as linearity, accuracy, LOD, LOQ, precision, and % relative standard deviation(RSD), were inside the appropriate limits. The HPLC was validated using all necessary variables with a successful estimation of mangiferin.

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“…The method for estimating piperine content using UV-spectrophotometric method was similar to that described above. [25][26][27][28] Samples were subjected to acid, base, oxidation, and photolytic degradation in order to evaluate the stability of the established UV-Spectroscopic approach as per ICH Q1A (R2) guidelines. The percentage degradation was estimated.…”

Section: Preparation Of Black Pepper and Commercialmentioning

confidence: 99%

UV-spectrophotometric Method Development and Validation for Piperine Estimation in Black Pepper, Ayurvedic Formulation and Novel Nano Formulation: A Perfect Quality Assessment Tool

Kalgonda Patil,

Jalalpure,

Chimagave

et al. 2023

Ind. J. Pharm. Edu. Res

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Aim:The current study aims to establish and validate a UV-spectrophotometric approach for estimating piperine in black pepper, commercial Ayurvedic preparation, and novel piperine-loaded nanoformulation. Materials and Methods: The UV-spectrophotometric technique was developed using methanol as a solvent. Piperine showed the absorbance at 342 nm. In accordance with ICH Q2 (R1) guidelines, the developed method was extensively validated in terms of selectivity, linear range, precision, accuracy, robustness, ruggedness, and reproducibility. The new method was also effectively used for precise estimation of piperine in black pepper, commercial Ayurvedic preparation and novel piperine loaded nanoformulation. The stability indicating study of the established UV-spectroscopic approach was carried as per as per ICH Q1A (R 2 ) guidelines. Results: The optimal parameters for piperine analysis were established using methanol as solvent. With the line equation y = 0.067x + 0.005, the maximum absorption wavelength was found to be 342 nm. Between concentration ranges of 0.5-32µg/ mL, it showed a linear response. The linear regression coefficient was found to be 0.999. The method's linearity, accuracy, ruggedness, specificity, and sensitivity were all validated, and all validation results were confirmed to be in acceptable ranges. Piperine was found to be stable and less prone to degradation under acidic, basic, thermal and photolytic stress conditions whereas oxidation degradation showed extremely degradable characteristics. Conclusion: The established approach will serve as standard quality control tool for precise estimation of piperine in herbal formulations and can be used for quality analysis.

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A single robust stability-indicating RP-HPLC analytical tool for apigenin quantification in bulk powder and in nanoliposomes: a novel approach

Cited by 6 publications

References 22 publications

Physicochemical and Antioxidant Properties of Nanoliposomes Loaded with Rosemary Oleoresin and Their Oxidative Stability Application in Dried Oysters

Physicochemical and Antioxidant Properties of Nanoliposomes Loaded with Rosemary Oleoresin and Their Oxidative Stability Application in Dried Oysters

High-performance liquid chromatography method development and validation for the quantification of mangiferin in Coffea arabica leaves

UV-spectrophotometric Method Development and Validation for Piperine Estimation in Black Pepper, Ayurvedic Formulation and Novel Nano Formulation: A Perfect Quality Assessment Tool

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