A Single Residue Change in
            <i>Vibrio harveyi</i>
            Hemolysin Results in the Loss of Phospholipase and Hemolytic Activities and Pathogenicity for Turbot (
            <i>Scophthalmus maximus</i>
            )

Sun, Bo; Zhang, Xiao‐Hua; Tang, Xuexi; Wang, Shushan; Zhong, Yueyang; Chen, Jixiang; Austin, Brian

doi:10.1128/jb.01650-06

Cited by 47 publications

(34 citation statements)

References 26 publications

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“…Carbohydrates showed high absorbencies in the region 1200-950cm -1 , that is within the so-called fingerprint region, where the position and intensity of the bands are specific for every polysaccharide, allowing it possible identification. These results are harmony with (Sun et al, 2007) Figure11-FTIR spectroscopy analysis of K. oxytoca polysaccharide extracted showing the transmittance trough at different wave number.…”

Section: Fourier Transform Infrared Spectroscopy (Ftir)supporting

confidence: 72%

Media Optimization for Exopolysaccharide Producing Klebsiella oxytoca KY498625 under Varying Cultural Conditions

Moghannem¹,

Farag²,

Shehab³

et al. 2017

Int. J. Adv. Res. Biol. Sci

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The present work was designed for studying the efficiency of multi-factorial experimental designs to elucidate factors affecting exopolysaccharide production by K.oxytoca isolated from soil. Combinations of Plackett-Burman design (PBD) and BoxBehnken design (BBD) have been carried out for media optimization of various factors. Among 11 factors, yeast extract, molasses and incubation temperature were the most significant factors. These three factors were selected for further optimization using Box-Behnken design (BBD). The result obtained from data analysis showed that; the most optimum concentrations were yeast extract 6%, molasses12% and incubation temperature 37 0 C. Bacterial isolate was identified as Klebsiella oxytoca using 16 S rRNAgene sequences. This isolate was submitted to the gene bank with accession number KY498625.Biopolymer was purified using DEAE-cellulose and identified using Fourier transform infrared (FTIR), gel permeation chromatography (GPC) and Gas mass chromatography spectroscopy (GC-MS).The result showed that; average molecular weight (Mw) of 4.05 x10 4 Da; major monosaccharide constituents were mannose, glucose, Arabinose and Galacturonic acid.

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Section: Fourier Transform Infrared Spectroscopy (Ftir)supporting

confidence: 72%

Media Optimization for Exopolysaccharide Producing Klebsiella oxytoca KY498625 under Varying Cultural Conditions

Moghannem¹,

Farag²,

Shehab³

et al. 2017

Int. J. Adv. Res. Biol. Sci

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“…Haemolysins are among the major exotoxins identified in Harveyi clade vibrios. Although generally referred to as haemolysins for their capacity to lyse erythrocytes, these toxins correspond to two different modes of action, either by forming pores or by breaking down cell membranes (Sun et al. 2007).…”

Section: Virulence Factors Of Vibrios Belonging To the Harveyi Cladementioning

confidence: 99%

Pathogenesis, virulence factors and virulence regulation of vibrios belonging to the Harveyi clade

Ruwandeepika

Jayaweera

Bhowmick

et al. 2012

Reviews in Aquaculture

127

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The Harveyi clade or core group of the Vibrio genus includes both pathogenic and non-pathogenic bacteria. Some of the species belonging to the clade, such as Vibrio harveyi, Vibrio alginolyticus, Vibrio campbellii and Vibrio parahaemolyticus, include serious pathogens of aquatic organisms. Importantly, within these species, some strains are pathogenic, whereas others are not. Other members of the clade, such as Vibrio natriegens and Vibrio mytili are reported to be non-pathogenic. As bacteria belonging to the Harveyi clade show a high diversity with respect to virulence and as the virulent members cause high mortalities in the aquaculture sector, it is important to understand which gene products are responsible for the pathogenicity of the bacteria and how expression of these virulence factors is regulated. This knowledge will ultimately allow for the development of biocontrol measures that aim to reduce the severe losses that are currently faced in the aquaculture sector as a result of vibriosis. In this review, we discuss the pathogenesis of bacteria belonging to the Harveyi clade, their impact on the aquaculture sector, the virulence factors that are involved in pathogenicity and their regulation

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“…Vibrio species that are pathogenic for fish and crustaceans secrete acyl hydrolases from the SGNH esterase family that likely play a role in pathogenicity (93). The V. harveyi hemolysin VHH is a PLB active against PC, hemolytic for fish erythrocytes, cytotoxic for fish cells, and upon intraperitoneal injection in flounder induces hemorrhage and death (93)(94)(95).…”

Section: Acyl Hydrolases: Phospholipases a And Bmentioning

confidence: 99%

“…The V. harveyi hemolysin VHH is a PLB active against PC, hemolytic for fish erythrocytes, cytotoxic for fish cells, and upon intraperitoneal injection in flounder induces hemorrhage and death (93)(94)(95).…”

Section: Acyl Hydrolases: Phospholipases a And Bmentioning

confidence: 99%

Bacterial Sphingomyelinases and Phospholipases as Virulence Factors

Flores-Dı́az

Monturiol-Gross

Naylor

et al. 2016

Microbiol Mol Biol Rev

165

143

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SUMMARYBacterial sphingomyelinases and phospholipases are a heterogeneous group of esterases which are usually surface associated or secreted by a wide variety of Gram-positive and Gram-negative bacteria. These enzymes hydrolyze sphingomyelin and glycerophospholipids, respectively, generating products identical to the ones produced by eukaryotic enzymes which play crucial roles in distinct physiological processes, including membrane dynamics, cellular signaling, migration, growth, and death. Several bacterial sphingomyelinases and phospholipases are essential for virulence of extracellular, facultative, or obligate intracellular pathogens, as these enzymes contribute to phagosomal escape or phagosomal maturation avoidance, favoring tissue colonization, infection establishment and progression, or immune response evasion. This work presents a classification proposal for bacterial sphingomyelinases and phospholipases that considers not only their enzymatic activities but also their structural aspects. An overview of the main physiopathological activities is provided for each enzyme type, as are examples in which inactivation of a sphingomyelinase- or a phospholipase-encoding gene impairs the virulence of a pathogen. The identification of sphingomyelinases and phospholipases important for bacterial pathogenesis and the development of inhibitors for these enzymes could generate candidate vaccines and therapeutic agents, which will diminish the impacts of the associated human and animal diseases.

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A Single Residue Change in Vibrio harveyi Hemolysin Results in the Loss of Phospholipase and Hemolytic Activities and Pathogenicity for Turbot ( Scophthalmus maximus )

Cited by 47 publications

References 26 publications

Media Optimization for Exopolysaccharide Producing Klebsiella oxytoca KY498625 under Varying Cultural Conditions

Media Optimization for Exopolysaccharide Producing Klebsiella oxytoca KY498625 under Varying Cultural Conditions

Pathogenesis, virulence factors and virulence regulation of vibrios belonging to the Harveyi clade

Bacterial Sphingomyelinases and Phospholipases as Virulence Factors

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