A simultaneous microwave-assisted extraction and adsorbent treatment process under acidic conditions for recovery and separation of paclitaxel from plant cell cultures

Kim, Gun-Joong; Kim, Jin-Hyun

doi:10.1007/s11814-015-0075-1

Cited by 21 publications

(5 citation statements)

References 22 publications

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“…Ustiloxins are acidic compounds due to the carboxyl and sulfonyl groups in the molecular structure, so an acidic solvent would improve the extraction performance. As in many cases, the acidic compounds were extracted with an acidic solvent (Beldean-Galea et al 2015; da Rocha and Norena 2020; Kim and Kim 2015). Herein, we screened six water-based extraction solvents and selected the 1% HCOOH solution because it gave the best extraction capacity.…”

Section: Discussionmentioning

confidence: 99%

Extraction and purification of ustiloxin A from rice false smut balls by a combination of macroporous resin and high-speed countercurrent chromatography

Wang

Zhao

et al. 2020

Food Prod Process and Nutr

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Rice false smut is an emerging plant disease worldwide. Ustiloxin A (UstA) is the major mycotoxin found in rice false smut balls, which are fungal colonies in rice florets. In this study, a new method consisting of macroporous resin column chromatography and high-speed countercurrent chromatography (HSCCC) was developed for UstA separation. UstA was extracted by a 3.81% HCOOH solution and adsorbed by XAD-4 resin. UstA was then eluted by a 40% methanol solution supplemented with 0.1% trifluoroacetic acid (TFA). Further purification was achieved by HSCCC using a two-phase solvent system consisting of n-butanol/TFA/H2O (1/0.05/1, v/v/v). Under the optimized conditions, 225 mg of UstA was obtained with a purity of 97.39% in a single run, with a final recovery of 65.2%. An inhibitory effect on seed germination of wheat and maize caused by UstA was observed in a preliminary phytotoxicity assay. Graphical abstract

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Section: Discussionmentioning

confidence: 99%

Extraction and purification of ustiloxin A from rice false smut balls by a combination of macroporous resin and high-speed countercurrent chromatography

Wang

Zhao

et al. 2020

Food Prod Process and Nutr

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“…Based on the previous experiments, 70% EtOH was considered the optimum solution for dissolved ILs. It was also reported that the volume concentration of EtOH was an important parameter affecting the extraction efficiency [45], and a high-volume concentration of EtOH was good for the extraction of the target analytes [46,47]. In Figure 3b, the extraction yield significantly increased with increasing the volume concentration of EtOH ( p < 0.01), and remained basically unchanged when it increased to 80%.…”

Section: Resultsmentioning

confidence: 70%

Ionic Liquid-Based Ultrasonic-Assisted Extraction to Analyze Seven Compounds in Psoralea Fructus Coupled with HPLC

et al. 2019

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Psoralea Fructus is widely used in traditional Chinese medicine (TCM), and the content of psoralen, isopsoralen, neobavaisoflavone, bavachin, psoralidin, isobavachalcone, and bavachinin A is the main quality control index of Psoralea Fructus because of its clinical effects. Thus, a fast and environmentally-benign extraction method of seven compounds in Psoralea Fructus is necessary. In this work, an ionic liquid-based ultrasonic-assisted method (ILUAE) for the extraction of seven compounds from Psoralea Fructus was proposed. Several ILs of different types and parameters, including the concentration of ILs, concentration of ethanol (EtOH), solid–liquid ratio, particle size, ultrasonic time, centrifugal speed, and ultrasonic power, were optimized by the Placket–Burman (PB) design and Box–Behnken response surface analysis. Under this optimal condition, the total extraction yield of the seven compounds in Psoralea Fructus was 18.90 mg/g, and significantly greater than the conventional 75% EtOH solvent extraction.

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“…Actually, there are many small molecule solvents, such as methanol, ethanol, acetone, dichloromethane, and trichloromethane, which were chosen for the extraction of paclitaxel from Taxus; methanol was proved to be the best solvent for the extraction among these solvents. Moreover, it was also reported that a high concentration of methanol (>90%) was more beneficial for the extraction [ 32 , 33 , 34 ]. The effect of methanol concentration in the range of 50–90 vol % was studied.…”

Section: Resultsmentioning

confidence: 99%

Ultrasonic Assisted Extraction of Paclitaxel from Taxus x media Using Ionic Liquids as Adjuvants: Optimization of the Process by Response Surface Methodology

et al. 2017

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(1) Background: Ionic liquids (ILs) are considered “green” solvents and have been widely used in the extraction and separation field in recent years; (2) Methods: In this study, some common ILs and functionalized magnetic ionic liquids (MILs) were used as adjuvants for the solvent extraction of paclitaxel from Taxus x media (T. x media) using methanol solution. The extraction conditions of methanol concentration, IL type and amount, solid–liquid ratio, extraction temperature, and ultrasonic irradiation time were investigated in single factor experiments. Then, three factors of IL amount, solid–liquid ratio, and ultrasonic irradiation time were optimized by response surface methodology (RSM); (3) Results: The MIL [C4MIM]FeCl3Br was screened as the optimal adjuvant. Under the optimization conditions of 1.2% IL amount, 1:10.5 solid–liquid ratio, and 30 min ultrasonic irradiation time, the extraction yield reached 0.224 mg/g; and (4) Conclusions: Compared with the conventional solvent extraction, this ultrasonic assisted extraction (UAE) using methanol and MIL as adjuvants can significantly improve the extraction yield, reduce the use of methanol, and shorten the extraction time, which has the potentiality of being used in the extraction of some other important bioactive compounds from natural plant resources.

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A simultaneous microwave-assisted extraction and adsorbent treatment process under acidic conditions for recovery and separation of paclitaxel from plant cell cultures

Cited by 21 publications

References 22 publications

Extraction and purification of ustiloxin A from rice false smut balls by a combination of macroporous resin and high-speed countercurrent chromatography

Extraction and purification of ustiloxin A from rice false smut balls by a combination of macroporous resin and high-speed countercurrent chromatography

Ionic Liquid-Based Ultrasonic-Assisted Extraction to Analyze Seven Compounds in Psoralea Fructus Coupled with HPLC

Ultrasonic Assisted Extraction of Paclitaxel from Taxus x media Using Ionic Liquids as Adjuvants: Optimization of the Process by Response Surface Methodology

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