A Simultaneous Determination of Honokiol and Magnolol in Oriental Pharmaceutical Decoctions Containing Magnolia Bark by Ion-Pair High-Performance Liquid Chromatography

Ueda, Joji; Momma, Noriko; Ohsawa, Keisuke

doi:10.1248/yakushi1947.113.2_155

Cited by 6 publications

(5 citation statements)

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“…and other reports (Ueda et al, 1993;Lee et al, 1995;Sheu and Lu, 1995;Tsai and Chen, 1992;Chou et al, 1996). Moreover, the simple composition of the mobile phase also made it easier to conduct analysis than ionpairing HPLC (Ueda et al, 1993). Although the HPLC-ED (Akira et al, 2005) method provided more specific detection and improved sensitivity than conventional HPLC-UV, it took 40 min to complete a single run, which is much longer than the present method.…”

Section: Applicationsmentioning

confidence: 75%

“…Basal levels were 0.0042 ± 0.002 µg/mL for honokiol and 0.0083 ± 0.04 µg/mL for magnolol. and other reports (Ueda et al, 1993;Lee et al, 1995;Sheu and Lu, 1995;Tsai and Chen, 1992;Chou et al, 1996). Moreover, the simple composition of the mobile phase also made it easier to conduct analysis than ionpairing HPLC (Ueda et al, 1993).…”

Section: Applicationsmentioning

confidence: 95%

“…Thin-layer chromatography (TLC) and gas chromatography (GC) were used to examine the distribution of honokiol and magnolol in plants (Fujita et al, 1973). Ion-pairing HPLC with UV detection using 10 mM tetra-n-amylammonium bromide and acetonitrile as a mobile phase was used to measure these two components in oriental pharmaceutical decoctions containing Magnolia bark (Ueda et al, 1993). HPLC with UV or photodiode-array detection (DAD) has also been applied to determine honokiol and magnolol in pharmaceutical preparations (Lee et al, 1995;Sheu and Lu, 1995;Tsai and Chen, 1992).…”

Section: Honokiolmentioning

confidence: 99%

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Simultaneous determination of honokiol and magnolol in Magnolia officinalis by liquid chromatography with tandem mass spectrometric detection

Wu¹,

Lin

Tsai

2006

Biomedical Chromatography

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An optimized high-performance liquid chromatographic method coupled with tandem mass spectrometric detection (LC-MS/MS) was developed for the simultaneous determination of honokiol and magnolol in Magnolia officinalis. Honokiol and magnolol were separated from the extracts using a reversed-phase C(18) column with a mobile phase consisted of acetonitrile and water (75:25, v/v) at a flow-rate of 0.8 mL/min. Selected reaction monitoring (SRM) mode was used for all sample quantification by the precursor-ion/product ion pair m/z 265 --> m/z 224 for honokiol and m/z 265 --> m/z 247 for magnolol. Validation data showed that this method has good linearity (r(2) > 0.995) over the concentration range of 0.0025-0.5 microg/mL for honokiol and magnolol, and both intra- and inter-day variability were acceptable within 15% at the lowest concentrations for this method. This proposed method provides excellent specificity, higher sensitivity and shorter run time than conventional methods and was applied successfully to determine the contents of honokiol and magnolol in M. officinalis.

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Section: Applicationsmentioning

confidence: 75%

Section: Applicationsmentioning

confidence: 95%

Section: Honokiolmentioning

confidence: 99%

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Simultaneous determination of honokiol and magnolol in Magnolia officinalis by liquid chromatography with tandem mass spectrometric detection

Wu¹,

Lin

Tsai

2006

Biomedical Chromatography

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“…Several methods for determination of magnolol and honokiol in Magnoliae Cortex have been described in the literature. These include ion-pair high-performance liquid chromatography [9], capillary electrophoresis with electrochemical detection [10], and supercritical-fluid extraction with on-line on-column trapping and supercritical-fluid chromatography [11]. HPLC is, however, still the most commonly used method for determination of magnolol and honokiol in Magnoliae Cortex [12,13].…”

Section: Introductionmentioning

confidence: 99%

HPLC analysis of magnolol and honokiol inMagnoliae Cortexafter solvent sublation

Dong¹,

Liu²

2008

Acta Chromatographica

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A novel method for separating and concentrating magnolol and honokiol from Magnoliae Cortex by solvent sublation and analysis of the compounds by highperformance liquid chromatography (HPLC) has been established. The optimum conditions for solvent sublation were use of n-butanol as sublation solvent, sample solution at pH 2, nitrogen flow 50 mL min −1 , and sublation time 50 min. The floating product obtained under the optimum conditions was determined by HPLC analysis on a C 18 reversed-phase column, with 22:78 (%, v/v) water-methanol as isocratic mobile phase at a flow rate of 1.00 mL min −1 . When the method was used for quantification of magnolol and honokiol in Magnoliae Cortex recovery ranged from 98.1 to 106.1%, RSD was from 3.07 to 4.80%, and LOD for honokiol and magnolol were 0.94 and 1.14 ng mL −1 , respectively.

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“…Several methods that have been reported for the determination of honokiol or magnolol are: ion-pair high-performance liquid chromatography (HPLC), 13) HPLC with UV detection (HPLC-UV), [14][15][16][17][18][19] and capillary zone electrophoresis (CZE). 20) However, these methods lack the sensitivity and selectivity required for determining contents of honokiol and magnolol in a small part of a sample tree.…”

mentioning

confidence: 99%

Determination of Honokiol and Magnolol by Micro HPLC with Electrochemical Detection and Its Application to the Distribution Analysis in Branches and Leaves of Magnolia obovata

et al. 2005

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The stem bark of Magnolia obovata (M. obovata) or Magnolia officinalis (M. officinalis) has been used in traditional Japanese herbal medicine, called Magnolia Bark in the Japanese Pharmacopoeia, for the treatment of thrombotic stroke, typhoid fever, fever, and headaches.1) It has been reported that Magnolia Bark suppresses mitogen-induced proliferation of human peripheral blood lymphocytes 2) and has central depressant effects.3) Honokiol and magnolol (structures shown in Fig. 1), isomers of neolignans, have been isolated from the bark of this plant and other Magnoliaceae. 4)These compounds have been found to exhibit muscle relaxant activity, 5) to inhibit intracellular calcium mobilization in platelets caused by collagen, even in the presence of indomethacin, 6) to relax vascular smooth muscles by releasing an endothelium-derived relaxing factor and to inhibit calcium influx through voltage-gated calcium channels, 7) and to have antihemostatic and antithrombotic effects.8) Recent studies indicate that honokiol has an antagonistic effect on calmodulin 9) and magnolol has anti-inflammatory and analgesic effects.10) In addition, honokiol has been reported to induce calcium mobilization, and to show neurotrophic activity in rat cortical neurons. 11,12) Thus the quality control of Magnolia Bark by determining honokiol and magnolol is important for pharmaceutical companies in the production of traditional Japanese herbal medicines. According to the Chinese Pharmacopoeia, the branch bark, root bark, flower buds, as well as the stem bark of M. officinalis var. biloba are also used for medicinal purposes. The distribution of honokiol and magnolol is significant for harvest of these crude herbal medicines in the optimal season, and/or for the selection of trees.Several methods that have been reported for the determination of honokiol or magnolol are: ion-pair high-performance liquid chromatography (HPLC), 13) HPLC with UV detection (HPLC-UV), [14][15][16][17][18][19] and capillary zone electrophoresis (CZE).20) However, these methods lack the sensitivity and selectivity required for determining contents of honokiol and magnolol in a small part of a sample tree. Electrochemical detection (ECD) is respected for its high sensitivity and selective determination of honokiol and magnolol, because it is both selective and sensitive for redox compounds such as phenolic compounds. Yet, no paper has been published on the determination of honokiol and magnolol by HPLC with ECD (HPLC-ECD).In our previous reports, we successfully developed a more than 30 fold sensitive HPLC-ECD method for determining catechins 21) and quercetin 22) in human plasma, and orthophenylphenol in lemon rind 23) using a microbore octadecylsilica (ODS) column, when compared to a reported HPLC-ECD method that used a conventional ODS column. [21][22][23] So, the present HPLC-ECD method using a microbore column (mHPLC-ECD) was expected to be an even more highly sensitive method. In the present study, we developed a mHPLC-ECD method for determining honokiol and m...

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A Simultaneous Determination of Honokiol and Magnolol in Oriental Pharmaceutical Decoctions Containing Magnolia Bark by Ion-Pair High-Performance Liquid Chromatography

Cited by 6 publications

References 0 publications

Simultaneous determination of honokiol and magnolol in Magnolia officinalis by liquid chromatography with tandem mass spectrometric detection

Simultaneous determination of honokiol and magnolol in Magnolia officinalis by liquid chromatography with tandem mass spectrometric detection

HPLC analysis of magnolol and honokiol inMagnoliae Cortexafter solvent sublation

Determination of Honokiol and Magnolol by Micro HPLC with Electrochemical Detection and Its Application to the Distribution Analysis in Branches and Leaves of Magnolia obovata

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