A simplified technique for the culture of amniotic fluid cells

Gray, C. T. H.; Davidson, Ronald G.; Cohen, Maimon M.

doi:10.1016/s0022-3476(71)80070-7

Cited by 25 publications

(3 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A culture surface coated with cell-free AF should be used immediately. This study also partially confirms an earlier observation made by Gray et al (1971) that AF stored for 1 month at room temperature and for 2 months at -20°C seems to have no appreciable growth effect on amniocytes when used in the culture medium.…”

Section: Discussionsupporting

confidence: 91%

Enhancement of amniocyte growth on a precoated surface

Chang

Jones

1991

Prenatal Diagnosis

View full text Add to dashboard Cite

This study investigates whether cell-free amniotic fluid facilitates cell attachment to the surface of culture plates and thereby promotes rapid amniocyte growth. Isolated or pooled cell-free amniotic fluid samples at different volumes were added to culture plates. Trypsinized subcultures, grown in Eagle's minimum essential alpha medium supplemented with fetal bovine serum (4-20 per cent), were monitored by cell counts. The results demonstrated growth stimulation on culture plates precoated with amniotic fluid. The minimal time for coating the culture plates was 6 h. Maximal coating was observed after an overnight incubation with 2-3 ml of the fluid per culture vessel. No synergistic effect from addition of fetal bovine serum to amniotic fluid was observed. A freshly coated surface provided the best amniocyte growth. When primary cultures are grown on a precoated surface, there is an increase in colony counts in 80 per cent of the samples tested. This method may be used to improve amniocyte growth, especially in samples with relatively small numbers of cells.

show abstract

Section: Discussionsupporting

confidence: 91%

Enhancement of amniocyte growth on a precoated surface

Chang

Jones

1991

Prenatal Diagnosis

View full text Add to dashboard Cite

show abstract

“…Since almost all the serum is imported this could well happen again. Our attempts to grow cells in amniotic fluid failed and this was also the experience of other authors (Gray, Davidson, and Cohen, 1971).…”

Section: Culture Mediasupporting

confidence: 56%

The selection of a method for the culture of cells from amniotic fluid.

Chitham¹,

Quayle²,

Hill³

1973

J Clin Pathol

View full text Add to dashboard Cite

“…Amniotic fluid cells obtained by amniocentesis were cultured as previously described (12). Control cell culture samples known to be chromosomally normal were obtained from pregnancies monitored previously for chromosomal abnormalities.…”

Section: Fetal Cells a N D Tissuesmentioning

confidence: 99%