The selection of a method for the culture of cells from amniotic fluid.

Chitham, R. G.; Quayle, Sarah; Hill, Lyndon M.

doi:10.1136/jcp.26.9.721

Search citation statements

Order By: Relevance

Paper Sections

Select...

Methods1

Citation Types

Supporting

Mentioning

Contrasting

Year Published

1975

2016

Publication Types

Select...

Article3

Book3

Relationship

Self Cite0

Independent6

Authors

Journals

Cited by 8 publications

(1 citation statement)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Cultures were performed according to the method of Chitham et al (1973) which in summary involves using human pooled A B Serum, Culture Medium 199, and a small amount of chick embryo extract, and subculture at the time of the addition of trypsin for harvesting. Cells from any one patient were grown in more than one culture vessel.…”

Section: Methodsmentioning

confidence: 99%

Fetal chromosome abnormalities detected in mid pregnancy

Craft

Hill²,

Rg³

1976

Int J Clinical Practice

View full text Add to dashboard Cite

Section: Methodsmentioning

confidence: 99%

Fetal chromosome abnormalities detected in mid pregnancy

Craft

Hill²,

Rg³

1976

Int J Clinical Practice

View full text Add to dashboard Cite

Aseptically collected calf serum as an effective alternative to fetal calf serum in the culture of amniotic fluid cells

et al. 1982

View full text Add to dashboard Cite

The growth-promoting activities of three different bovine sera have been compared in primary and secondary amniotic fluid cell cultures. In secondary amniotic fluid cell microcultures, aseptically collected calf serum (CS) was slightly, though not significantly more effective than fetal calf serum (FCS) in promoting DNA synthesis, while newborn calf serum (NCS) was significantly less effective than either CS or FCS. All three sera were optimally effective at concentrations of 10 per cent (v/v). Significant variation in quality occurred within four batches of each of CS and FCS, but not within four batches of NCS. A selected batch of CS was significantly more effective in promoting the growth of primary amniotic fluid cell cultures than were a number of batches of FCS then in routine laboratory use. It is suggested that CS may serve as an effective and economical alternative to FCS in the culture of amniotic fluid cells, thereby expanding the scope of serum batch testing. A possible explanation for the varying growth-promoting activities of different sera is discussed.

show abstract

Cell Culture Studies

Amsterdam

1975

The Gangliosidoses

View full text Add to dashboard Cite

The selection of a method for the culture of cells from amniotic fluid.

Cited by 8 publications

References 6 publications

Fetal chromosome abnormalities detected in mid pregnancy

Fetal chromosome abnormalities detected in mid pregnancy

Aseptically collected calf serum as an effective alternative to fetal calf serum in the culture of amniotic fluid cells

Cell Culture Studies

Contact Info

Product

Resources

About