A simplified method for the purification of an intrinsically disordered coagulant protein from defatted  Moringa oleifera  seeds

Dezfooli, Seyedehsara Masoomi; Uversky, Vladimir N.; Saleem, Mussarat; Baharudin, Farah Salma; Hitam, Sharifah Mariam Sayed; Bachmann, Robert Thomas

doi:10.1016/j.procbio.2016.04.021

Cited by 45 publications

(10 citation statements)

References 32 publications

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“…This extraction step could be followed by purification steps to isolate protein that acts as active coagulating agent. Several purification methods have been investigated, such as ion exchange [13][14][15], dialysis [16], spray drying [17], ultrafiltration [18,19], and lyophilization [20]. However, the previously mentioned methods require many complicated steps, making it impractical in large scale.…”

Section: Introductionmentioning

confidence: 99%

Adsorbed leucaena protein on citrate modified Fe3O4 nanoparticles: synthesis, characterization, and its application as magnetic coagulant

Kristianto

Reynaldi

Prasetyo

et al. 2020

Sustain Environ Res

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Natural coagulants from plants resources have gained a lot of attention as it is renewable, biodegradable, non-hazardous, lower cost, and less sludge generated compared to chemical coagulants. However there are still some drawbacks, namely long settling time and possible increase of dissolved organic carbon in the treated water. In this paper we tried to address these drawbacks by utilizing citrate modified Fe3O4 to adsorb protein from Leucaena leucocephala as the active coagulating agent. The effect of trisodium citrate concentration and protein adsorption pH to the adsorbed protein was investigated. It was found that the trisodium citrate concentration of 0.5 M and pH 4.0 gave the highest protein adsorption. The obtained magnetic coagulant was furthermore characterized using Scanning Electron Microscopy, X-ray Diffraction, Fourier Transform Infrared Spectroscopy, and Transmission Electron Microscopy to observe the characteristics before and after protein adsorption. Furthermore, the effect of pH (2 to 10) and coagulant dosage (60 to 600 mg L− 1) to the removal of synthetic Congo red wastewater and sludge volume formation was investigated. It was found that pH 3 was the best pH for coagulation due to charge neutralization mechanism of leucaena protein. Furthermore the highest removal was obtained at dosage 420 mg L− 1 with 80% removal. This result was comparable with crude extract of leucaena with half settling time (20 min) and lower increase of permanganate value, indicating lower increase of dissolved organics in the treated water.

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Section: Introductionmentioning

confidence: 99%

Adsorbed leucaena protein on citrate modified Fe3O4 nanoparticles: synthesis, characterization, and its application as magnetic coagulant

Kristianto

Reynaldi

Prasetyo

et al. 2020

Sustain Environ Res

View full text Add to dashboard Cite

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“…Esta metodologia simples apresenta algumas desvantagens, como a introdução de mais matéria orgânica na água a ser tratada (que favorece a formação de subprodutos de desinfecção ao utilizar cloro gasoso como agente desinfetante) e a diminuição da eficiência de coagulação provocada pela presença de lipídeos e carboidratos nas sementes. 79 A extração e purificação das proteínas de interesse são estratégias que podem ser adotadas para otimizar a ação coagulante de Moringa oleífera. Metodologias combinadas, tais como a extração de proteínas em tampão fosfato e posterior purificação por cromatografia de troca iônica, resultaram em um produto proteico ativo de elevada massa molecular (66 kDa).…”

Section: Moringa Oleíferaunclassified

“…80 A precipitação proteica utilizando sulfato de amônio ((NH4)2SO4) e posterior purificação por diálise e tratamento térmico também foi aplicada, resultando na obtenção de proteínas coagulantes termicamente estáveis, mesmo quando aquecidas a 121 ºC. 79,81 As aplicações de Moringa oleífera como coagulante de águas e efluentes são extensamente relatadas no meio acadêmico. 76,82,83,84 Um manual prático (escrito em linguagem simples) para o uso da espécie como coagulante de águas em comunidades rurais está disponível na literatura.…”

Section: Moringa Oleíferaunclassified

Natural Products Used as Coagulants and Flocculants for Public Water Supply: A Review of Benefits and Potentialities

Júnior¹,

Abreu²

2018

Rev. Virtual Quim.

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Abstract:The development of new coagulants and flocculants, based on nature-abundant biodegradable natural raw materials, has been gaining more space in research centers as an environmental technology that follows the concept of eco friendly materials and the principles of green chemistry proposed in the 1990s. When well exploited, these multifunctional agents can reduce the operational cost of water treatment and expand access to this resource. In view of the promising prospects for the use of natural products as sources of biocomposites, this review aims to compile the several studies developed in recent years that report on the obtaining and efficiency of natural materials such as coagulants and flocculants of water for supply, with emphasis on the chitosan, Moringa oleifera seeds and vegetable tannins. In parallel, it is also intended to expose the limitations and harms of traditional coagulants based on inorganic salts and synthetic polymers, as well as their mechanisms of action, in contrast to the main physico-chemical parameters used in the quality control of natural and treated waters.Keywords: Natural products; Green chemistry; Natural coagulants; Water treatment. ResumoO desenvolvimento de novos coagulantes e floculantes, baseados em matérias-primas naturais biodegradáveis abundantes na natureza, vem ganhando cada vez mais espaço nos centros de pesquisa como uma tecnologia ambiental que segue o conceito de materiais eco friendly e os princípios da química verde propostos na década de 90. Quando bem explorados, esses agentes multifuncionais podem permitir a redução do custo operacional do tratamento de água e expandir o acesso a esse recurso. Em face as perspectivas promissoras do uso de produtos naturais como fontes de biocompostos, esta revisão tem como objetivos compilar os diversos estudos desenvolvidos nos últimos anos que relatam a obtenção e eficiência de materiais naturais como agentes coagulantes e floculantes de águas para abastecimento, com ênfase na quitosana, sementes da espécie Moringa oleífera e taninos vegetais. Em paralelo, pretende-se também expor as limitações e malefícios dos coagulantes tradicionais a base de sais inorgânicos e polímeros sintéticos, bem como seus mecanismos de atuação, contrastando com os principais parâmetros físico-químicos utilizados no controle de qualidade de águas naturais e tratadas.Palavras-chave: Produtos Naturais; Química verde; Coagulantes naturais; Tratamento de águas.

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“…This extraction step could be followed by puri cation steps to isolate protein that acts as active coagulating agent. Several puri cation methods have been investigated, such as ion exchange [13][14][15], dialysis [16], spray drying [17], ultra ltration [18,19], and lyophilization [20]. However, the previously mentioned methods require many complicated steps, making it impractical in large scale.…”

Section: Introductionmentioning

confidence: 99%

Adsorbed leucaena protein on citrate modified Fe3O4 nanoparticles: synthesis, characterization, and its application as magnetic coagulant

Kristianto

Reynaldi

Prasetyo

et al. 2020

Preprint

View full text Add to dashboard Cite

Natural coagulants from plants resources have gained a lot of attention as it is renewable, biodegradable, non-hazardous, lower cost, and less sludge generated compared to chemical coagulants. However there are still some drawbacks, namely long settling time and possible increase of dissolved organic carbon in the treated water. In this paper we tried to address these drawbacks by utilizing citrate modified Fe3O4 to adsorb protein from Leucaena leucocephala as the active coagulating agent. The effect of trisodium citrate concentration and protein adsorption pH to the adsorbed protein was investigated. It was found that the trisodium citrate concentration of 0.5 M and pH 4.0 gave the highest protein adsorption. The obtained magnetic coagulant was furthermore characterized using Scanning Electron Microscopy, X-ray Diffraction, Fourier Transform Infrared Spectroscopy, and Transmission Electron Microscopy to observe the characteristics before and after protein adsorption. Furthermore, the effect of pH (2 to 10) and coagulant dosage (60 to 600 mg L-1) to the removal of synthetic Congo red wastewater and sludge volume formation was investigated. It was found that pH 3 was the best pH for coagulation due to charge neutralization mechanism of leucaena protein. Furthermore the highest removal was obtained at dosage 420 mg L-1 with 80% removal. This result was comparable with crude extract of leucaena with half settling time (20 min) and lower increase of permanganate value, indicating lower increase of dissolved organics in the treated water.

show abstract

A simplified method for the purification of an intrinsically disordered coagulant protein from defatted Moringa oleifera seeds

Cited by 45 publications

References 32 publications

Adsorbed leucaena protein on citrate modified Fe3O4 nanoparticles: synthesis, characterization, and its application as magnetic coagulant

Adsorbed leucaena protein on citrate modified Fe3O4 nanoparticles: synthesis, characterization, and its application as magnetic coagulant

Natural Products Used as Coagulants and Flocculants for Public Water Supply: A Review of Benefits and Potentialities

Adsorbed leucaena protein on citrate modified Fe3O4 nanoparticles: synthesis, characterization, and its application as magnetic coagulant

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