A simplified in vitro model for investigation of the antimicrobial efficacy of various antiseptic agents to prevent peri-implantitis

Abstract

The biofilm formation by oral bacteria on the implant surface is one of the most remarkable factors of peri-implant infections, which may eventually lead to bone resorption and loss of the dental implant. Therefore, the elimination of biofilm is an essential step for the successful therapy of implant-related infections. In this work we created a basic in vitro model to evaluate the antibacterial effect of three widely used antiseptics. Commercially pure (CP4) titanium sample discs with sand blasted, acid etched, and polished surface were used. The discs were incubated with mono-cultures of Streptococcus mitis and Streptococcus salivarius. The adhered bacterial biofilms were treated with different antiseptics: chlorhexidine-digluconate (CHX), povidone-iodine (PI), and chlorine dioxide (CD) for 5 min and the control discs with ultrapure water. The antibacterial effect of the antiseptics was tested by colorimetric assay. According to the results, the PI and the CD were statistically the most effective in the elimination of the two test bacteria on both titanium surfaces after 5 min treatment time. The CD showed significant effect only against S. salivarius. Based on our results we conclude that PI and CD may be promising antibacterial agents to disinfecting the peri-implant site in the dental practice.

Effects of different decontaminating solutions used for the treatment of peri-implantitis on the growth of Porphyromonas gingivalis-an in vitro study

“…Peri-implant inflammation and lesions are of multifactorial nature, but it is a fact unequivocally supported by the literature that P. gingivalis plays a leading role in both cases [24,50,51]. In a previous study, it was demonstrated that P. gingivalis has the ability to colonize titanium surface in multiple interconnected layers, forming a biofilm, namely a polysaccharide coat (glycocalyx), which is a protective layer for the bacterial population [52,53].…”

Surface Free Energy and Composition Changes and Ob Cellular Response to CHX-, PVPI-, and ClO2-Treated Titanium Implant Materials

“…The antibacterial efficiency of these solutions was proven by our research group previously [ 44 ]. Venkei et al found that 5 min treatment of PVPI and ClO 2 led to significant elimination of Streptococcus mitis and Streptococcus salivarius in their in vitro study.…”

The systemic oral health connection: Biofilms

“…[ 41 ] Additional evidence that the use of chlorine dioxide oral rinses, available OTC in most drug stores or supermarket with other oral care products reduces oral biofilm intraorally and rinsing removable prosthetics (dentures) in this eliminates biofilm decreasing aspiration potential. [ 42 , 43 ] Therefore, placing all elderly patients on chlorhexidine or chlorine dioxide daily rinses as a preventive measure should be recommended to aid in preventing potential aspiration pneumonia. This should be a more predictable approach in elderly patients who lack manual dexterity to perform oral homecare with a toothbrush.…”