A simplified guide for charged aerosol detection of non-chromophoric compounds—Analytical method development and validation for the HPLC assay of aerosol particle size distribution for amikacin

Soliven, Arianne; Ahmad, Imad A. Haidar; Tam, James P.; Kadrichu, Nani; Challoner, P; Markovich, Robert J.; Blaskó, Andrei

doi:10.1016/j.jpba.2017.05.013

Cited by 30 publications

(8 citation statements)

References 22 publications

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“…HPLC-CAD has been used widely for the quantitation of analytes including in botanical research (Baker and Regg, 2018;Poplawska et al, 2018;Soliven et al, 2017;Vervoort et al, 2008). However, depending on the type of sample, the selectivity of the CAD may or may not be sufficient to quantitate constituents in a complex mixture.…”

Section: Quantitation Of E Purpurea Constituents By Hplc-cad and Lc-ms/msmentioning

confidence: 99%

A strategy for test article selection and phytochemical characterization of Echinacea purpurea extract for safety testing

Waidyanatha

Pierfelice

Cristy

et al. 2020

Food and Chemical Toxicology

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Botanical dietary supplements (BDS) are used around the world for many purported therapeutic properties. The selection of an authentic product and it's phytochemical characterization is critical to generate robust safety data. Because botanicals are complex mixtures with variable quality, identification of a representative product for testing has been challenging. Echinacea is used for its purported immune stimulant properties and was listed as the 2 nd top-selling BDS in 2018. However, there are limited safety data for Echinacea. Hence, the National Toxicology Program (NTP) has selected Echinacea for safety testing using rodent models. Here, we describe selection and comprehensive characterization of an Echinacea purpurea root extract to be used in the NTP testing program. Using non-targeted chemical analyses combined with chemometric analysis, a potential unfinished product (i.e., an extract that serves as source material for finished products) of Echinacea purpurea was selected. The product was then authenticated using chemical and DNA techniques and characterized, including the phytochemical composition. Among numerous constituents identified, caftaric acid, chicoric acid, chlorogenic acid and dodeca-2(E),4(E),8(Z),10(E/Z)-tetraenoic acid isobutylamide made up a small fraction of the extract. Based on these analyses, an approach is proposed for test article selection for Echinacea research which can be adapted to other botanicals.

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Section: Quantitation Of E Purpurea Constituents By Hplc-cad and Lc-ms/msmentioning

confidence: 99%

A strategy for test article selection and phytochemical characterization of Echinacea purpurea extract for safety testing

Waidyanatha

Pierfelice

Cristy

et al. 2020

Food and Chemical Toxicology

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“…However, high-performance liquid chromatography (HPLC) plays an important role in the determination of antibacterial drugs in body fluids. A wide spectrum of detectors used for determination (UV, DAD, PDA, FL, MS as well as universal detectors: CAD or ELSD) and modern methods of sample preparation for the analysis enabled to obtain reproducible results, even in complex matrices [57][58][59][60][61][62][63][64][65][66][67]. Nonetheless, LC-MS/MS is the only technique that ensures unambiguous analysis.…”

Section: Liquid Chromatography (Lc)mentioning

confidence: 99%

Determination and Identification of Antibiotic Drugs and Bacterial Strains in Biological Samples

2020

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Antibiotics were initially natural substances. However, nowadays, they also include synthetic drugs, which show their activity against bacteria, killing or inhibiting their growth and division. Thanks to these properties, many antibiotics have quickly found practical application in the fight against infectious diseases such as tuberculosis, syphilis, gastrointestinal infections, pneumonia, bronchitis, meningitis and septicemia. Antibiotic resistance is currently a detrimental problem; therefore, in addition to the improvement of antibiotic therapy, attention should also be paid to active metabolites in the body, which may play an important role in exacerbating the existing problem. Taking into account the clinical, cognitive and diagnostic purposes of drug monitoring, it is important to select an appropriate analytical method that meets all the requirements. The detection and identification of the microorganism responsible for the infection is also an essential factor in the implementation of appropriate antibiotic therapy. In recent years, clinical microbiology laboratories have experienced revolutionary changes in the way microorganisms are identified. The MALDI-TOF MS technique may be interesting, especially in some areas where a quick analysis is required, as is the case with clinical microbiology. This method is not targeted, which means that no prior knowledge of the infectious agent is required, since identification is based on a database match.

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“…All these detection methods have some limitations for use. 4 RI detection slightly varies from gradient methods which are required to separate the analogue from its main components, CAD and ECD detectors are very sensitive to small changes in a specified temperature. 5,6 Monitoring of known related substances in routine analysis will provide the exact measurement of the true value during manufacturing.…”

Section: Introductionmentioning

confidence: 99%

Implementation of Total Error Perception in the Analytical Method Validation for Quantification of Related Substance in Pharmaceutical Formulation

Sofiqul¹,

Murugan²,

Premakumari³

2021

JYP

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Objectives: This study is initiated to develop a simple and reproducible liquid chromatographic method with ultraviolet detection for the estimation of Sisomicin in Gentamicin pharmaceutical formulation by application of total error statistical tool. Methods: Gentamicin has a weak UV chromophore it is not possible to detect low levels of known related substance of gentamicin using a UV detector. Hence derivatization technique was applied to detect those substances. Chromatographic separation was accomplished by using Thermo scientific Hypersil Gold column (150 x 4.6 mm) and 5µm particle size as stationary phase by isocratic elution with Methanol: Water: Glacial acetic acid: Sodium hexane sulfonate in the ratio 70:25:5:3% v/v/ v/w as mobile phase. Sisomicin was detected at 330 nm within 25 min with a flow rate of 0.5 mL/min. Results: Concurrent results were obtained in the developed analytical method based on total error measurement. Proposed method showed good linearity response (r> 0.995) with limit of quantification at 0.007495 mg/mL, % relative standard deviation less than 1% in repeatability and % recovery was found within 96 to 98 % in accuracy. Accuracy profile result found within the range of ±10% and risk profile ±5% between the two set. Conclusion: This method can separate all the analogues of Gentamicin including known related substances. The finding demonstrated that method could be suitable for quantification of related substances in parental liquid dosage form.

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A simplified guide for charged aerosol detection of non-chromophoric compounds—Analytical method development and validation for the HPLC assay of aerosol particle size distribution for amikacin

Cited by 30 publications

References 22 publications

A strategy for test article selection and phytochemical characterization of Echinacea purpurea extract for safety testing

A strategy for test article selection and phytochemical characterization of Echinacea purpurea extract for safety testing

Determination and Identification of Antibiotic Drugs and Bacterial Strains in Biological Samples

Implementation of Total Error Perception in the Analytical Method Validation for Quantification of Related Substance in Pharmaceutical Formulation

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