A simplified and accurate method for the analysis of urinary metabolites of testosterone‐related steroids using gas chromatography/combustion/isotope ratio mass spectrometry

Ouellet, Alexandre; LeBerre, Nicolas; Ayotte, Christiane

doi:10.1002/rcm.6620

Cited by 18 publications

(39 citation statements)

References 41 publications

(187 reference statements)

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“…The compounds monitored were all the compounds included in the steroid module of the ABP. The 13 C delta values of the target compounds (TC) testosterone (T), epitestosterone (E), androsterone (A), etiocholanolone (Etio), 5α-androstan-3α,17β-diol (5aAdiol), 5β-androstan-3α,17βdiol (5bAdiol) were determined. [12] Briefly, 3 to 6mL of urine depending on the concentrations established after the application of the method for the establishment of the SP were extracted as previously described.…”

Section: Methodsmentioning

confidence: 99%

“…[11][12][13] The stability of the IRMS parameters of healthy individuals in terms of short (1month), intermediate (6months) and long-term (1year) variability was studied in our laboratory [14] and observed by others. [11][12][13] The stability of the IRMS parameters of healthy individuals in terms of short (1month), intermediate (6months) and long-term (1year) variability was studied in our laboratory [14] and observed by others.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Longitudinal evaluation of the isotope ratio mass spectrometric data: towards the ‘isotopic module’ of the athlete biological passport?

Jardines

Botrè

Colamonici

et al. 2016

Drug Testing and Analysis

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The detection of the abuse of pseudo-endogenous steroids (testosterone and/or its precursors) is currently based on the application of the steroid module of the World Anti-Doping Agency (WADA) Athletes' Biological Passport (ABP), implemented through ADAMS. Diagnostic metabolites are monitored for every athlete and statistically evaluated with a predictive Bayesian approach. In the case of suspicious samples, the data of the ABP are confirmed and the isotope ratio mass spectrometry (IRMS) test is activated. We have previously demonstrated that IRMS enables confirmation of the non-endogenous origin of pseudo-endogenous steroids in otherwise non-suspicious samples, after a longitudinal evaluation of the ABP, even after the inclusion of additional long-term diagnostic hydroxylated metabolites, and that the delta values of the parameters obtained during the IRMS confirmation process presented much less variability compared to the parameters of the ABP. The aim of the present work is to evaluate the application of the same methodology used for the evaluation of the ABP, on the delta values of the pseudo-endogenous steroids monitored. The effectiveness of the proposed model has been assessed on samples obtained after controlled administrations of oral androstenedione and transdermal testosterone. The results support the conclusion that, if applied, the longitudinal evaluation of the IRMS data allows the detection of positive samples that otherwise will be reported as atypical findings (ATF), improving the efficacy of the fight against doping in sport. This approach, by narrowing the individual acceptable range, could possibly improve the detection of the intake of preparations of synthetic origin with delta values close to or overlapping those of endogenously produced steroids. Copyright © 2016 John Wiley & Sons, Ltd.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Longitudinal evaluation of the isotope ratio mass spectrometric data: towards the ‘isotopic module’ of the athlete biological passport?

Jardines

Botrè

Colamonici

et al. 2016

Drug Testing and Analysis

View full text Add to dashboard Cite

show abstract

“…Nowadays, a methodology based on determining the percentage of 13 C in the molecule, namely, GC with combustion isotope ratio mass spectrometry (GC-C-IRMS), is normally applied to ascertain if an atypical content of endogenous steroids is due to either its intake by an athlete or physiological factors [7,8].…”

Section: Introductionmentioning

confidence: 99%

Development of an analytical method for the determination of the misuse in sports of boldenone through the analysis of urine by on-line coupling liquid chromatography–gas chromatography–combustion–isotope ratio mass spectrometry

Toledano

Dı́az-Plaza²,

Cortés

et al. 2014

Journal of Chromatography A

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“…The described confirmatory methods [12][13][14][15][16] are laborious, requiring a purification step by liquid chromatography in order to guarantee extracts of adequate purity suitable for IRMS analysis, precluding the application of the method on a large scale.…”

Section: Introductionmentioning

confidence: 99%

Fast IRMS screening of pseudoendogenous steroids in doping analyses

Torre

Colamonici

Curcio

et al. 2017

Drug Testing and Analysis

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The detection of the abuse of pseudoendogenous steroids (testosterone and/or its precursors) is currently based, when possible, on the application of the steroid module of the World Anti-Doping Agency (WADA), athlete biological passport (ABP), implemented through the global database, ADAMS. When a suspicious sample is detected, the confirmation by isotope ratio mass spectrometry (IRMS) is required. It is well known that this confirmation procedure is time consuming and expensive and can be only applied on a reduced number of samples. In previous studies we have demonstrated that the longitudinal evaluation of the IRMS data is able to detect positive samples that otherwise will be evaluated as negative, improving the efficacy of the fight against doping in sport. This would require the analysis of a much larger volume of samples by IRMS. The aim of the present work is to describe an IRMS screening method allowing to increase the throughput of samples that can be analyzed by IRMS. The detection efficacy of the method is compared with the confirmation method in use, and to assess its robustness and applicability, all the samples of a major cycling stage competition were analyzed, with the agreement of the testing authority, under routine conditions and response times. The results obtained permit to conclude that the IRMS screening method here proposed has adequate selectivity and produces results that overlap with the already validated method currently in use permitting to analyze a much higher volume of samples even during a major event without compromising the detection capacity.

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A simplified and accurate method for the analysis of urinary metabolites of testosterone‐related steroids using gas chromatography/combustion/isotope ratio mass spectrometry

Cited by 18 publications

References 41 publications

Longitudinal evaluation of the isotope ratio mass spectrometric data: towards the ‘isotopic module’ of the athlete biological passport?

Longitudinal evaluation of the isotope ratio mass spectrometric data: towards the ‘isotopic module’ of the athlete biological passport?

Development of an analytical method for the determination of the misuse in sports of boldenone through the analysis of urine by on-line coupling liquid chromatography–gas chromatography–combustion–isotope ratio mass spectrometry

Fast IRMS screening of pseudoendogenous steroids in doping analyses

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