A simplification of the protein assay method of Lowry et al. which is more generally applicable

Peterson, Gary L.

doi:10.1016/0003-2697(77)90043-4

Cited by 8,271 publications

(2,991 citation statements)

References 19 publications

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“…Cell debris was removed through centrifugation (5 min, 14 000 g) and the protein concentration in the supernatant was determined by a modified Folin-Lowry method. 40 The gel shift assay system from Promega was used and EMSAs were carried out according to the manufacturer's recommendations. An oligonucleotide probe containing the -light chain enhancer NF B consensus binding site (5Ј-AGT TGA GGG GAC TTT CCC AGG C-3Ј) was end-labeled with 32 P-ATP (Amersham, Little Chalfont, UK).…”

Section: Gene Therapymentioning

confidence: 99%

Anti-inflammatory gene therapy directed at the airway epithelium

Griesenbach¹,

Scheid²,

Hillery³

et al. 2000

Gene Ther

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Section: Gene Therapymentioning

confidence: 99%

Anti-inflammatory gene therapy directed at the airway epithelium

Griesenbach¹,

Scheid²,

Hillery³

et al. 2000

Gene Ther

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“…To quantify the lipid peroxidation in tissues, the level of malondialdehyde (MDA) was determined spectrophotometrically after reaction with thiobarbituric acid (TBA) by the modified method of Ohkawa (Ohkawa, Ohishi, & Yagi, 1979). Protein content in each sample was determined by the Lowry method with Peterson modification (Lowry, Rosebrough, Farr, & Randall, 1951; Peterson, 1977) and the concentration of TBARS (thiobarbituric acid reactive substances) was expressed in nmol/mg protein. The damage to proteins was quantified by measuring protein carbonyls by spectrophotometry after reaction with 2,4‐dinitrophenylhydrazine (DNPH) (Levine, Williams, Stadtman, & Shacter, 1994; Stadtman & Oliver, 1991).…”

Section: Methodsmentioning

confidence: 99%

“…The total glutathione concentration in liver was measured according to Anderson (1996) along with modification for use with a 96‐well plate reader (Vasilaki et al., 2006) and expressed in nmol per mg protein. In both analyses, protein content was measured with the Lowry method with Peterson modification (Lowry et al., 1951; Peterson, 1977). …”

Section: Methodsmentioning

confidence: 99%

Oxidative damage and antioxidant defense are assay and tissue‐dependent both in captive and wild‐caught bank voles (Myodes glareolus) before and after reproduction

Ołdakowski

Taylor

2018

Ecology and Evolution

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Reproduction is costly and life‐history theory predicts that current parental investment will result in lower survival or decreased future reproduction. The physiological mechanisms mediating the link between reproduction and survival are still under debate and elevated oxidative damage during reproduction has been proposed as a plausible candidate. Previous studies of oxidative stress during reproduction in animals under natural conditions have been restricted to analyses of blood. Herein, we measured the level of oxidative damage to lipids (tiobarbituric‐acid‐reactive substances) and proteins (carbonyls) in the liver, kidneys, heart and skeletal muscles in free‐living bank vole females from spring and autumn generations, before and after reproduction. Antioxidant defense in the liver and kidneys was also determined. We expected oxidative damage to tissues and hypothesized that the damage would be more uniform between tissues in wild animals compared to those breeding under laboratory conditions. Considering all combinations of markers/tissues/generations, oxidative damage in females did not differ before and after reproduction in 12 comparisons, was lower after reproduction in three comparisons, and was higher after breeding in one comparison. The total glutathione was significantly increased after reproduction only in the liver of the autumn generation and there was no change in catalase activity. Our results confirm—for the first time in the field—previous observations from laboratory studies that there is no simple link between oxidative stress and reproduction and that patterns depend on the tissue and marker being studied. Overall, however, our study does not support the hypothesis that the cost of reproduction in bank voles is mediated by oxidative stress in these tissues.

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“…At 48 h after transfection, the medium was removed and frozen at À801C for VEGF quantification. The protein concentration in each well was determined using a modified Folin-Lowry assay 17 and the VEGF concentration was normalized for total protein content.…”

Section: In Vitro Transfectionmentioning

confidence: 99%

“…The protein concentration in tissue homogenates was determined using a modified Folin-Lowry assay. 17 For VEGF serum measurements, total blood was spun at 4000 rpm for 10 min and the serum was removed and frozen at À801C. VEGF was measured in tissue lysates and serum using a human VEGF ELISA (R&D Systems, Abingdon, UK) according to the manufacturer's protocol.…”

Section: Vegf and Cat Detection In Myocardial Tissue And Serummentioning

confidence: 99%