A simple zeta method for sperm selection based on membrane charge

Chan, Philip J.; Jacobson, John D.; Corselli, J.; Patton, William C.

doi:10.1016/j.fertnstert.2005.07.1302

Cited by 108 publications

(88 citation statements)

References 46 publications

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“…Total sperm motility and velocities were unaffected by the brief exposure to the serum-free condition or the manipulation from the attaching-detaching process suggesting a lack of association between these parameters and zeta potential [1]. Note that the sperm fraction selected by the zeta process consisted of both motile sperm and non-motile sperm with net negative membrane charge.…”

Section: Discussionmentioning

confidence: 93%

“…The data suggested that the cryopreservation process, which involved low temperatures and exposure to glycerol and egg yolk protein, did not impact the sperm membrane net zeta potential. This was important as the crux of the zeta potential method was the negative electrokinetic charge required for selection [1,9].…”

Section: Discussionmentioning

confidence: 99%

“…The zeta selection process [1] is defined as an electrostatic procedure that selects for sperm with a net negative zeta potential charge due to acquired epididymal proteins localized on the membrane surface. The initial step in the experiment design consisted of equally dividing each washed sperm specimen into two portions.…”

Section: Zeta Potential Sperm Selection Processmentioning

confidence: 99%

“…One portion was cryopreserved as outlined below while the remaining portion was subjected to zeta processing. Zeta processing [1] was carried out immediately to avoid confounding factors due to capacitation-related changes in the sperm membrane electropotential [16,17]. The washed sperm from each specimen was pipetted into a new centrifuge tube and diluted with 5 ml of serum-free HEPES-HTF medium.…”

Section: Zeta Potential Sperm Selection Processmentioning

confidence: 99%

“…A recent report demonstrated improvements in washed sperm quality after selecting for negatively charged sperm using a zeta potential processing method [1]. Specifically, the percentages of sperm with strict normal morphology, hyperactive motility and forward progression almost doubled in the zeta processed sperm group.…”

Section: Introductionmentioning

confidence: 96%

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Retention of membrane charge attributes by cryopreserved-thawed sperm and zeta selection

Kam

Jacobson

Patton

et al. 2007

J Assist Reprod Genet

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Purpose Mature sperm can be selected based on their negative zeta electrokinetic potential. The zeta selection of cryopreserved sperm is unknown. The objective was to study the effect of zeta processing on the morphology and kinematic parameters of cryopreserved-thawed sperm. Methods Colloid-washed sperm (N=9 cases) were cryopreserved for 24 h, thawed and diluted in serum-free medium in positive-charged tubes. After centrifugation, the tubes were decanted, serum-supplemented medium was added and the resuspended sperm were analyzed. Untreated sperm and fresh sperm served as the controls. Results There were improvements in strict normal morphology in fresh (11.8±0.3 versus control 8.8±0.3 %, mean ±SEM) and thawed (8.7±0.2 versus control 5.4±0.2%) sperm after zeta processing. Percent sperm necrosis was reduced after zeta processing (66.0±0.6 versus unprocessed 74.6±0.3%). Progression decreased by 50% but not total motility after zeta processing of thawed sperm. Conclusions The results suggested that the cryopreservation process did not impact the sperm membrane net zeta potential and higher percentages of sperm with normal strict morphology, acrosome integrity and reduced necrosis were recovered. The zeta method was simple and improved the selection of quality sperm after cryopreservation but more studies would be needed before routine clinical application.

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Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Section: Zeta Potential Sperm Selection Processmentioning

confidence: 99%

Section: Zeta Potential Sperm Selection Processmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 96%

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Retention of membrane charge attributes by cryopreserved-thawed sperm and zeta selection

Kam

Jacobson

Patton

et al. 2007

J Assist Reprod Genet

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Sperm Selection Techniques and their Relevance toART

2016

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Review on Sperm Sorting Technologies and Sperm Properties toward New Separation Methods via the Interface of Biochemistry and Material Science

et al. 2019

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Successful fertilization in mammals requires spermatozoa to efficiently traverse the female reproductive tract to meet the egg. This process naturally selects high quality sperm cells for fertilization, but when artificial reproductive technologies are used such as in vitro fertilization, intracytoplasmic sperm injection, or intrauterine insemination, other methods of sperm selection are required. Currently, technology enables sperm sorting based on motility, maturity as defined by zeta potential or hyaluronic acid binding site expression, absence of apoptotic factors, appropriate morphology, and even sex. This review summarizes current knowledge on all known methods of sperm cell sorting, compares their efficiency, and discusses the advantages and limitations of each technique. Scope for further refinement and improvement of current methods are discussed as is the potential to utilize a variety of materials to innovate new methods of sperm separation.

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A simple zeta method for sperm selection based on membrane charge

Cited by 108 publications

References 46 publications

Retention of membrane charge attributes by cryopreserved-thawed sperm and zeta selection

Retention of membrane charge attributes by cryopreserved-thawed sperm and zeta selection

Sperm Selection Techniques and their Relevance toART

Review on Sperm Sorting Technologies and Sperm Properties toward New Separation Methods via the Interface of Biochemistry and Material Science

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