Sperm Selection Techniques and their Relevance to
            <scp>ART</scp>

Lee, Simon; Shamsi, Monis Bilal; Carrell, Douglas T.

doi:10.1002/9781118849613.ch1

Cited by 2 publications

(7 citation statements)

References 116 publications

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“…The binding to hyaluronic acid is reported to mediate some intracellular signalling pathways, leading to increased motility (Bains, Miles, Carson, & Adeghe, 2001). The expression of hyaluronic acid receptors can be used as a biomarker to select and isolate mature spermatozoa for ART (Parmegiani et al, 2010;Simon, Shamsi, et al, 2016). There are two techniques based on hyaluronic acid-mediated sperm selection, namely the physiological intracytoplasmic sperm injection (PICSI) and the hyaluronan (or hyaluronic acid)-rich medium.…”

Section: Hyaluronic Acid-mediated Sperm Selectionmentioning

confidence: 99%

“…At the end of the MACS protocol, two distinct fractions are obtained: Annexin-negative (nonapoptotic spermatozoa with intact membrane) and Annexin-positive (apoptotic spermatozoa with externalised phosphatidylserine; Glander & Schaller, 1999;Grunewald et al, 2001). As there are other components in semen that may decrease the efficiency of this technique, the association of this method with a standard sperm wash technique, such as density gradient centrifugation, is recommended (Simon, Shamsi, et al, 2016). As an alternative to the magnetic beads, glass wool can be coated with Annexin V. This technique, commonly referred as Annexin-V glass wool or molecular glass wool, further improves the filtration process as apoptotic spermatozoa are bounded to the Annexin V. This technique was successfully used on both fresh and cryopreserved semen samples, although more studies are needed in order to validate its efficiency for clinical practice (Grunewald et al, 2007).…”

Section: Magnetic-activated Cell Sortingmentioning

confidence: 99%

“…The membrane of mature human spermatozoa possesses a zeta potential between −16 and −20 mV, which is conferred by a coating of sialic acids during spermatogenesis and epididymal maturation (Ishijima, Okuno, & Mohri, 1991;Kirchhoff & Hale, 1996). This negative potential can be used as a biomarker for the selection of mature sperm cells (Simon, Shamsi, & Carrell, 2016).…”

Section: Zeta Potentialmentioning

confidence: 99%

“…In fact, some sperm cells considered as normal in a conventional morphological analysis may present ultra-structural defects that negatively affect the ART outcome (Simon, Shamsi, et al, 2016). A new method for detailed morphological assessment of motile spermatozoa in real time was developed in 2002-motile sperm organelle morphology examination (MSOME).…”

Section: Motile Sperm Organelle Morphology Examinationmentioning

confidence: 99%

“…Annexin V is a phospholipid-binding protein with high affinity for phosphatidylserine. Phosphatidylserine is a negatively charged phospholipid that is present in the inner layer of the plasma membrane of both somatic and sperm cells (Glander & Schaller, 1999;Simon, Shamsi, et al, 2016), which is externalised to the outer layer of the cell during the early phases of apoptosis (Vermes, Haanen, Steffens-Nakken, & Reutelingsperger, 1995). Thus, phosphatidylserine is used as a biomarker for apoptotic sperm cells.…”

Section: Magnetic-activated Cell Sortingmentioning

confidence: 99%

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Sperm selection strategies and their impact on assisted reproductive technology outcomes

et al. 2020

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Assisted reproductive technologies (ART) revolutionised human fertility by providing treatment to patients previously considered incapable for reproductive purposes (Henkel & Schill, 2003). In 2017, it was estimated that approximately 6 million infants were born through ART since 1978, when the first baby produced by in vitro fertilisation (IVF) was born. It has been reported that about half of these births were in the past decade, highlighting the increasing use of these technologies, (Hunter, 2017). Initially, ART research was mainly focused on comprehending the factors that increase the likelihood of a successful pregnancy and

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Section: Hyaluronic Acid-mediated Sperm Selectionmentioning

confidence: 99%

Section: Magnetic-activated Cell Sortingmentioning

confidence: 99%

Section: Zeta Potentialmentioning

confidence: 99%

Section: Motile Sperm Organelle Morphology Examinationmentioning

confidence: 99%

Section: Magnetic-activated Cell Sortingmentioning

confidence: 99%

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Sperm selection strategies and their impact on assisted reproductive technology outcomes

et al. 2020

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Keeping Voices in the Room: Values Clarification in Codesign for Equitable Science and Technology Education

Dixon

Hsi

Doren

2022

Curator The Museum Journal

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When engaging issues at the intersection of science and society, science centers, museums and other informal STEM learning organizations struggle to center perspectives of communities most often impacted by the unequal distribution of technologies' benefits and harms. Increasingly, participatory design is being utilized to do this, but the field must continue to refine methods for accessing the expertise of community partners and keeping it present across multiple design stages and products. We share a case study in which a multi-institution project team, developing resources for educational programming around radio frequency technologies, worked with community design to establish a values foundation that could guide initial planning and ongoing development. We share design methods adapted from values sensitive design and equity-centered researchpractice partnership, as well as insights relevant to enacting design practice that can build relational equity, leverage data across institutional boundaries, and span locations, platforms and levels of expertise. Colin Dixon is a researcher and educator focussed on science learning across settings. Colin received a Ph.D. from the UC Davis School of Education. He works to understand and co-create learning experiences that are equitable, inclusive and centered around what young people want to do in their worlds. Sherry Hsi leads Making Waves with Radio at BSCS Science Learning. Her research aims to support inquirybased STEM, equity, and learner agency in museums and out-of-school settings. Dr. Hsi received her B.S., M.S., and Ph.D. degrees in Engineering and Science Education from the University of California, Berkeley. Seth Van Doren received his B.A. degree in Chemistry from the University of California Berkeley in 2019 and is currently pursuing a PhD at UC Irvine. He researches authentic STEM investigations in K-12 settings, social learning, and increasing student epistemic agency.

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Sperm Selection Techniques and their Relevance to ART

Cited by 2 publications

References 116 publications

Sperm selection strategies and their impact on assisted reproductive technology outcomes

Sperm selection strategies and their impact on assisted reproductive technology outcomes

Keeping Voices in the Room: Values Clarification in Codesign for Equitable Science and Technology Education

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