A simple technique using skin implants to produce histocompatability (BoLA) typing sera*

Pringnitz, Deborah J.; McLaughlin, Kyle; Benforado, Kathy; Strozinski, L.; Stone, W. H.

doi:10.1111/j.1365-2052.1982.tb01047.x

Cited by 13 publications

(2 citation statements)

References 10 publications

(4 reference statements)

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“…For some of the experiments described in the Results section, animals had reciprocal skin implantations (Pringnitz et al 1982). The animals were initially selected on the basis of BOLA class I identity (see below).…”

Section: Itnmutzizutiotimentioning

confidence: 99%

Alloreactive T‐cell recognition of bovine major histocompatibility complex class II products defined by one‐dimensional isoelectric focusing

et al. 1992

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T-cell recognition of bovine MHC (BoLA) class I1 antigens was investigated in relation to BoLA class I1 polymorphisms defined by one-dimensional isoelectric focusing (1D-IEF). One-way mixed lymphocyte reactions (MLRs), and allospecific cell lines and clones were used. In general, T-cell responses correlated with the 1D-IEF defined haplotypes (EDF types). However, with MLRs some responses appeared to be associated with BoLA class I differences. All combinations of responder-stimulator pairs produced alloreactive T-cell responses both in MLR and in generation of allolines/clones. Thus allospecific lines and clones were generated to all EDF types tested. Splits in the IEF typing were observed with EDF6 and EDF3, indicating that distinct BoLA class I1 haplotypes are not necessarily distinguished by 1D-IEF alone. Furthermore, the patterns of reactivity with EDF3 expressing cells were complex with the T-cell specificities splitting EDF3 into several distinct types. Also, in some cases it was clear that more than one T-cell specificity per EDF type was detectable. Thus, allospecific lines and clones provide complementary and additional information to the 1D-IEF typing for polymorphism of the BoLA class I1 complex. This extra information is particularly important in terms of the functional significance of the BoLA complex for antigen presentation and immune response gene effects.

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Section: Itnmutzizutiotimentioning

confidence: 99%

Alloreactive T‐cell recognition of bovine major histocompatibility complex class II products defined by one‐dimensional isoelectric focusing

et al. 1992

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“…Many workers have succeeded in producing sera against lymphocyte antigens in ruminant domestic animals, using different methods. The two most common procedures have been skin grafting (Ford 1974;Spooner et al 1978;van Dam 1981;Pringnitz et al 1982) and injection of isolated lymphocytes (Millot 1978;Spooner et al 1978;van Dam 1981;Amorena & Stone 1982). In addition, postpartum sera from non-injected animals have been reported to be a source of antilymphocyte antibodies (Ford & Elves 1974;Caldwell & Cumberland 1978;Newman & Hines 1979;van Dam 1981;Stear & Spooner 1983;Dufty & Outteridge 1985).…”

Section: Introductionmentioning

confidence: 99%

Production of alloantibodies against caprine lymphocyte antigens

Nesse

Larsen²

1987

Animal Genetics

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In all, 217 primiparous goats were each injected with blood cells from their own newborn kid. Eighty-five goats were given mononuclear cells, 61 were given leucocytes and 71 received whole blood. The goats were injected one, two or three times before collection of sera. Sera were also collected from 42 non-injected, primiparous goats. The sera were compared with regard to their potential value in class I histocompatibility typing. The percentage of potentially valuable sera was highest in the group of animals injected twice with whole blood (66.7%). However, this percentage was not significantly higher than the percentage in the group of animals injected once with whole blood (54.7%). It is concluded that injecting primiparous goats once with whole blood from their own newborn kid, is a rapid and easy method, which gives a high yield of alloantisera with potential value in class I histocompatibility typing.

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Preparation of B lymphocyte-specific alloantisera by skin implant immunization of cattle

Mackie

Stear

1992

Veterinary Immunology and Immunopathology

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A simple technique using skin implants to produce histocompatability (BoLA) typing sera*

Cited by 13 publications

References 10 publications

Alloreactive T‐cell recognition of bovine major histocompatibility complex class II products defined by one‐dimensional isoelectric focusing

Alloreactive T‐cell recognition of bovine major histocompatibility complex class II products defined by one‐dimensional isoelectric focusing

Production of alloantibodies against caprine lymphocyte antigens

Preparation of B lymphocyte-specific alloantisera by skin implant immunization of cattle

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