Preparation of B lymphocyte-specific alloantisera by skin implant immunization of cattle

Mackie, John T.; Stear, M. J.

doi:10.1016/0165-2427(92)90146-h

Cited by 1 publication

(1 citation statement)

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“…major histocompatibility complex (MHC) class-II specific alloantisera have been raised by planned immunization through injection of leucocytes or by subcutaneous skin implantation. Two strategies have been used: firstly, immunizations between 'class4 identical' and 'class-I1 non-identical' animals (Amens et al, 1991;Davies & Antczak 1991;Williams et al, 1991); and secondly, immunizations between 'class-I and class-II non-identical' animals, with subsequent platelet absorption of the sera to remove the anti-class-I reactivity (Newman et al, 1982;Mackie, 1990;Mackie & Stear 1992). Irrespective of the strategy used, only a limited number of class-I1 typing sera was produced.…”

Section: Introductionmentioning

confidence: 99%

Complexity of the Bovine MHC Class‐ii Specificity Dw3 as Defined by Alloantisera

Nilsson

Klooster

Poel

et al. 1994

Int J Immunogenetics

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Alloantisera related to the bovine major histocompatibility complex (MHC) class-II specificity Dw3 were investigated by cross-absorption experiments and by application of the monoclonal antibody-specific immobilization of lymphocyte antigen assay (MAILA). The absorption study revealed antibodies specific for an antigenic determinant shared by all Ds03 (Dw3)-positive animals, and several other antibody populations recognizing the locally defined specificities Ds10, Ds11 and Ds15, that are closely associated with Ds03. The results of the MAILA-assay indicate that the Ds03 specificity is probably encoded by DQ, whereas specificities Ds10 and Ds 11 are more closely associated with DR molecules. The data presented here provide the first evidence that bovine DR and DQ specificities can be identified separately by serological methods using alloimmune antisera.

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