A simple spectrophotometric method for the determination of urea in blood and urine

With, Torben K.; Petersen, T. D.; Petersen, Birte Brandt

doi:10.1136/jcp.14.2.202

Cited by 44 publications

(12 citation statements)

References 3 publications

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“…The Watt and Chisp (1954) method that utilized a modified Ehlich reagent detailed in With et al (1961) was followed. 32 All experiments were conducted in triplicate to ensure precision with analytical measurements made singly. The mean and standard deviation of the triplicate data was calculated and represented on the graphs.…”

Section: Methodsmentioning

confidence: 99%

Characterization of urea hydrolysis in fresh human urine and inhibition by chemical addition

Ray

Saetta

Boyer

2018

Environ. Sci.: Water Res. Technol.

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Section: Methodsmentioning

confidence: 99%

Characterization of urea hydrolysis in fresh human urine and inhibition by chemical addition

Ray

Saetta

Boyer

2018

Environ. Sci.: Water Res. Technol.

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“…Further, 2.5 mL of this filtrate was added to 2.5 mL of Erlich's reagent and 1.25 mL of water. The solution thus obtained was homogenously mixed and analyzed at 430 nm in a UV–visible spectrophotometer (Shimadzu UV‐1601, Japan) . The urea uptake capacity ( q e , mg g −1 ) was evaluated as mentioned in Eqn .…”

Section: Methodsmentioning

confidence: 99%

Influence of process parameters on sorption capacity of microwave activated biosorbents for urea recovery from human urine: a comparative study

Ganesapillai

Venugopal

Ananthkrishna

et al. 2015

Asia-Pacific J Chem Eng

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The present investigation elicits a comparative study for recovering urea from human urea via adsorption onto three biosorbents: Microwave Activated Carbonized Bamboo Shoots (MACBS), Walnut Shells (MACWS) and Coconut Shells (MACCS). The rate of sorption was investigated under different process parameters including initial sorbate concentration, amount of sorbent, temperature and agitation speed, and further, sorption capacity of MACBS, MACWS and MACCS was compared. Batch results indicated that urea uptake capacity increased with an increase in sorbate concentration and a decrease in sorbent loading and temperature. The experimental data were tested against the Langmuir, Freundlich, Tempkin, Flory-Huggins and Dubinin-Radushkevich (D-R) isotherms with the D-R model being a good fit for MACBS and MACCS and the Freundlich model for MACWS. Kinetic studies were performed to understand the order and rate of the sorption process. While pseudo-second-order kinetic model fits best for MACBS and MACWS, pseudofirst order was followed by MACCS. Based on the urea sorption capacity, the following order of suitability of precursors for activated carbon preparation was established: MACBS > MACCS > MACWS. This study illustrates the suitability of biomass-based precursors for activated carbon preparation and nutrient recovery from wastewater streams like human urine for subsequent use as fertilizers on agricultural land.

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“…The determination of the urea concentration released was analyzed by ultraviolet-visible spectroscopy (Shimadzu 1601 PC) with the method of With et al 18 The methodology consisted of the preparation of Ehrlich's reagent (5.0 g of dimethylbenzaldehyde plus 20 mL of hydrochloric acid and the completion of the volume to 100 mL with Sigma-Aldrich reagent) and a 10% solution of trichloroacetic acid (Sigma-Aldrich). The sample (500 mL) was mixed with 2.5 mL of the 10% trichloroacetic acid solution and 500 mL of Ehrlich reagent, and absorbance was obtained in the 400-500 nm range.…”

Section: Scanning Electron Microscopy (Sem)mentioning

confidence: 99%

Controlled release of nitrogen‐source fertilizers by natural‐oil‐based poly(urethane) coatings: The kinetic aspects of urea release

Bortoletto-Santos

Ribeiro

Polito

2016

J of Applied Polymer Sci

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Urea is the most common source of nitrogen fertilizer, but it has serious disadvantages with respect to volatilization and leaching; these can be controlled by polymeric coatings on the granules. However, the effectiveness of this strategy depends on the formed polymer presenting good adhesion, reactivity with the urea surface, uniformity, and efficient release kinetics control. Therefore, in this study, we evaluated the release kinetics of urea coated by polyurethane produced from two oils (soybean and castor) by analyzing the influence of the thickness, coating stability, deposition interface, and total release time. The results demonstrate that castor oil produced superior materials, which achieved a total release time in water immersion of over 40 days with a total coating mass of less than 4% of the total mass. The good interface suggested that this material may produce coatings of good quality and long release times with minimum thickness, and this will maximize the total nitrogen present in the granule. V C 2016 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2016, 133, 43790.

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A simple spectrophotometric method for the determination of urea in blood and urine

Cited by 44 publications

References 3 publications

Characterization of urea hydrolysis in fresh human urine and inhibition by chemical addition

Characterization of urea hydrolysis in fresh human urine and inhibition by chemical addition

Influence of process parameters on sorption capacity of microwave activated biosorbents for urea recovery from human urine: a comparative study

Controlled release of nitrogen‐source fertilizers by natural‐oil‐based poly(urethane) coatings: The kinetic aspects of urea release

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