A Simple Spectrophotometric Estimation of Tramadol Hydrochloride in Pharmaceutical Formulations

Abstract

A simple, accurate, rapid and sensitive spectrophotometric method has been developed for the determination of tramadol hydrochloride in pharmaceutical formulations. The method was based on the formation of chloroform extractable complex of tramadol hydrochloride with wool fast blue, which shows absorbance maxima at 590 nm against the reagent blank treated similarly. The method obeys Beer's law in the concentration ranges of 50-250 µg/mL. Validation studies are statistically significant as all the statistical parameters are within the acceptance range (% RSD< 2.0 and S.D. < 2.0) for both accuracy and precision study. High recovery and low % RSD reveals the reliability of the method for quantitative study of the proposed method in tablet formulation. The proposed method is simple, rapid accurate, precise, reproducible and economic and can be used for routine quantitative analysis of tramadol hydrochloride in pure and tablet dosage form.

Rp-HPLC Method Development and Validation of Tremadol Hydrochloride in Bulk Form by Ion-Pair Liquid Chromatography

“…It is used as an oral non-steroidal antiinflammatory drug with good analgesic and tolerability profile in various painful conditions. 1 In literature survey several methods like spectrophotometry [2][3][4][5][6][7] , HPTLC [8][9] and HPLC [10][11][12][13][14] . Most of the methods were on estimation of Tramadol HCl in biological samples.…”

Carbon Nanotube Electrodes in Comparison to Coated Platinum Wire and Carbon Paste Selective Electrodes for the Determination of Tramadol Hydrochloride in Bulk, Pharmaceutical Formulations, and Spiked Human Plasma and Urine

“…(2,3) Tramadol is applied mainly in the treatment of acute (e.g., postoperative, trauma) and chronic (cancer and non-cancer) pain. (4) Several analytical techniques have been mentioned for the determination of TR.HCl in pharmaceutical dosage forms and in biological fluids, such as spectrophotometry, (5)(6)(7)(8)(9) spectrofluorimetery, (10)(11)(12) high-performance liquid chromatography, (13)(14)(15) thin layer chromatography, (16) and electrochemical sensors. (17)(18)(19)(20)(21) The reported TR.HCl potentiometric sensors were based on the formation of an ion association complex between TR.HCl and either tetraphenyl borate, phosphotungestate, or phosphomolybedate for TR.HCl recognition and quantification.…”

Estimation of Tramadol Hydrochloride in Bulk and Formulation by Second Order Derivative Area Under Curve UV-Spectrophotometric Methods.

“…It is used to treat moderate to moderately severe pain and most types of neuralgia, including trigeminal neuralgia. Literature survey reveals that, several spectrophotometric method [1][2][3] TLC-densitometries [4], UV spectrophotometric and HPLC-DAD methods [5], HPLC method [6][7][8] High Performance thin layer chromatographydensitometry [9], have been reported for the estimation of tramadol in pharmaceutical formulations. Few analytical methods were reported in literature for the determination of tramadol and other combination drugs which includes spectrophotometric method [10][11][12], spectrophotometric and spectrofluorimetric method [13][14][15].…”