A Simple, Sensitive, Rapid and Specific Detection Method for Bisphenol A based on Fluorescence Polarization Immunoassay

Abstract

A rapid, sensitive, specific and simple method based on Fluorescence Polarization Immunoassay (FPIA) was developed for the quantitative detection of Bisphenol A in water. 4, 4-Bis (4-hydroxyphenyl) valeric acid (BHPVA) was selected as the hapten to produce the immunogen. Fluorescein-labeled Bisphenol A derivatives (tracers) with different structures were synthesized and purified by thin layer chromatography. Based on the polyclonal antibody and tracers, an optimized FPIA method was developed with a detection limit (10% inhibition) of 2 μg L(-1) and a linear working range of 20 to 800 μg L(-1). FPIA was suitable for screening a large number of samples. The recoveries of fortified Tai lake water samples ranged from 91.85 to 102.78%, and tap water samples ranged from 90.36 to 96.01%. The coefficients of variation were all less than 20%. This FPIA method, which did not require a complicated cleanup process, proved to be very useful for the screening of Bisphenol A in environmental water samples.

A fluorescence polarization immunoassay method for detection of the bisphenol A residue in environmental water samples based on a monoclonal antibody and 4′-(aminomethyl)fluorescein

“…Generally, high-performance liquid chromatography (HPLC) and gas chromatography (GC) are accurate and reliable but expensive, time-consuming and unsuitable for analysis of a large amount of sample. 22 Therefore, there is a big demand to develop another sensitive FPIA for determination of BPA based on a new uorescein and sensitive monoclonal antibody. ELISA 19 and CLEIA 1 are heterogeneous methods which need 2-3 h and several times of washing, while FPIA is a homogeneous method which needs no separation or washing steps but just a short time of 10 min.…”

“…20,21 Wu et al had set up a FPIA method based on uorescein (BVA-EDF) and polyclonal antibody, and the paper was published on Immunological Investigations. 22 Therefore, there is a big demand to develop another sensitive FPIA for determination of BPA based on a new uorescein and sensitive monoclonal antibody.…”

Development of a Lateral Flow Immunoassay (LFIA) to Screen for the Release of the Endocrine Disruptor Bisphenol A from Polymer Materials and Products

“…The anti-bisphenol A mouse monoclonal antibody was provided by the lab of Professor Chuanlai Xu [18] (School of Food Science & Technology, State Key Lab of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, China), dissolved in a PBS-based buffer (7 mg/mL in 0.01 M PBS, pH 7.4, 1% BSA, 1% glycerol, 0.02% azide). The polyclonal goat anti-mouse HRP-labeled antibody (clone A4416) (whole molecule, 0.5-3 mg/mL in 0.01 M PBS, pH 7.4, 1% BSA, 0.02% azide) and sheep anti-mouse IgG, 0.6-2 mg/mL in 0.01 M PBS, pH 7.4, 1% BSA, 0.02% azide) were provided by Merck Millipore (Darmstadt, Germany).…”

A camelid VHH-based fluorescence polarization immunoassay for the detection of tetrabromobisphenol A in water

“…A suitable binding of antibody with antigen can be easily affected by chemicals that are structurally related to analytes, causing false positive results. 36,38 A set of important TBBPA analogues such as tetrabromobisphenol A-bis(2,3-dibromopropylether) (TBBPA-DBPE), 2,2 0 ,6,6 0 -tetrabromobisphenol A diallyl ether (TBBPA-BAE), hexabromocyclododecane (HBCD), 1,2-bis(pentabromodiphenyl)ethane (DBDPE), polybrominated diphenyl ethers (PBDEs) and bisphenol A (BPA) were therefore used to evaluate the cross-reactivity. The FPIA exhibited little cross-reactivity with some tested compounds including BDE-99 (3.8%), BDE-47 (4.2%), TBBPA-DBPE (6.0%), TBBPA-BAE (7.1%) and BPA (8.2%) (Table 2).…”