A simple screening assay for receptor switching of avian influenza viruses

Suptawiwat, Ornpreya; Kongchanagul, Alita; Chan-it, Wisoot; Thitithanyanont, Arunee; Wiriyarat, Witawat; Chaichuen, Krisada; Songserm, Taweesak; Suzuki, Yasuo; Puthavathana, Pilaipan; Auewarakul, Prasert

doi:10.1016/j.jcv.2008.01.014

Cited by 27 publications

(12 citation statements)

References 12 publications

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“…18, 24 The α-2,6 or α-2,3-binding preference could be distinguished by the change of HA titer reacted with RBCs and enzymatic RBCs. Herein, we analyzed the receptor-binding specificity of the H6 isolates using HA assays with sialidase-treated TRBCs, which have only α-2,6 receptors.…”

Section: Resultsmentioning

confidence: 99%

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Molecular characterization of H6 subtype influenza viruses in southern China from 2009 to 2011

Zou

Gao

Zhang

et al. 2016

Emerging Microbes & Infections

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H6 avian influenza viruses (AIVs), which are prevalent in domestic and wild birds in Eurasian countries, have been isolated from pigs, a dog and a human. Routine virological surveillance at live poultry markets or poultry farms was conducted in southern China from 2009 to 2011. This study investigated the genetic and antigenic characteristics, analyzed the receptor-binding properties and evaluated the kinetics of infectivity of the AIVs in A549, MDCK and PK15 cells. A total of 14 H6N6 and 2 H6N2 isolates were obtained from four provinces in southern China. Genetic analysis indicated two distinct hemagglutinin lineages of the H6 strains cocirculating in southern China, and these strains facilitated active evolution and reassortment among multiple influenza virus subtypes from different avian species in nature. None of these isolates grouped with the novel Taiwan H6N1 virus responsible for human infection. Receptor-binding specificity assays showed that five H6 AIVs may have acquired the ability to recognize human receptors. Growth kinetics experiments showed that EV/HB-JZ/02/10(H6N2) and EV/JX/15/10(H6N6) initially reproduced faster and achieved higher titers than other viruses, suggesting that enhanced binding to α-2,6-linked sialic acids correlated with increased viral replication in mammalian cells. Overall, the results emphasize the need for continued surveillance of H6 outbreaks and extensive characterization of H6 isolates to better understand genetic changes and their implications.

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Section: Resultsmentioning

confidence: 99%

“…A HA assay using 1% TRBC, which was treated with α-2,3-specific sialidase, was performed as previously described 18 with minor modifications. Briefly, 10% TRBC suspension prepared in phosphate buffer solution was treated by 625 mU 2,3-specific sialidase (Takara, Dalian, China) at 37 °C for 30 min.…”

Section: Methodsmentioning

confidence: 99%

Molecular characterization of H6 subtype influenza viruses in southern China from 2009 to 2011

Zou

Gao

Zhang

et al. 2016

Emerging Microbes & Infections

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“…Several methods are applied for exploring the carbohydrate-binding preference of influenza viruses or recombinant HA, including cell-based assays [37][38][39], immobilization of virus [40][41][42][43][44][45][46] and glycan microarray technology [47][48][49]. In our study, 30 biotin-PAA-glycans (Table 4, most of them were sulfated and sialylated) were selected and fabricated on membrane and applied for the carbohydrate ligand surveillance of influenza clinical isolates.…”

Section: Surveillance Of Seasonal and Pandemic H1n1 Influenza A Virusmentioning

confidence: 99%

Carbohydrate Microarray

Chang¹

2012

Carbohydrates - Comprehensive Studies on Glycobiology and Glycotechnology

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“…RBCs from species such as chicken, turkey, horses, guinea pigs and humans have been used in such assays [18,19]. RBCs have also been used in conjunction with sialidases and sialyltransferase to present certain glycan structures, for example exclusively containing either α2-3 or α2-6 linked sialic acid [20,21]. This type of assay however is inherently limited in that it fails to account for receptor complexity beyond the sialic acid linkage, i.e.…”

Section: Introductionmentioning

confidence: 99%

Modular glycosphere assays for high-throughput functional characterization of influenza viruses

et al. 2013

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BackgroundThe ongoing global efforts to control influenza epidemics and pandemics require high-throughput technologies to detect, quantify, and functionally characterize viral isolates. The 2009 influenza pandemic as well as the recent in-vitro selection of highly transmissible H5N1 variants have only increased existing concerns about emerging influenza strains with significantly enhanced human-to-human transmissibility. High-affinity binding of the virus hemagglutinin to human receptor glycans is a highly sensitive and stringent indicator of host adaptation and virus transmissibility. The surveillance of receptor-binding characteristics can therefore provide a strong additional indicator for the relative hazard imposed by circulating and newly emerging influenza strains.ResultsStreptavidin-coated microspheres were coated with selected biotinylated glycans to mimic either human or avian influenza host-cell receptors. Such glycospheres were used to selectively capture influenza virus of diverse subtypes from a variety of samples. Bound virus was then detected by fluorescently labelled antibodies and analyzed by quantitative flow cytometry. Recombinant hemagglutinin, inactivated virus, and influenza virions were captured and analyzed with regards to receptor specificity over a wide range of analyte concentration. High-throughput analyses of influenza virus produced dose–response curves that allow for functional assessment of relative receptor affinity and thus transmissibility.ConclusionsModular glycosphere assays for high-throughput functional characterization of influenza viruses introduce an important tool to augment the surveillance of clinical and veterinarian influenza isolates with regards to receptor specificity, host adaptation, and virus transmissibility.

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A simple screening assay for receptor switching of avian influenza viruses

Cited by 27 publications

References 12 publications

Molecular characterization of H6 subtype influenza viruses in southern China from 2009 to 2011

Molecular characterization of H6 subtype influenza viruses in southern China from 2009 to 2011

Carbohydrate Microarray

Modular glycosphere assays for high-throughput functional characterization of influenza viruses

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