A simple route to functionalising electrospun polymer scaffolds with surface biomolecules

Dziemidowicz, Karolina; Brocchini, Steve; Williams, Gareth R.

doi:10.1016/j.ijpharm.2021.120231

Cited by 9 publications

(8 citation statements)

References 31 publications

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“…Fibrous nanoscale materials formed during electrospinning have a high porosity and a large specific surface area, which allows them to be used as highly efficient filters and absorbents [9]. Additionally, electrospun polymer materials could be easily modified with different biologically active compounds [10], biomolecules [11] and carbon nanomaterials such as carbon nano-onions [12,13] to provide the surface functionalization of polymeric electrospun scaffolds for drug delivery, regenerative medicine and tissue engineering.…”

Section: Introductionmentioning

confidence: 99%

Effect of Glycero-(9,10-trioxolane)-trialeate on the Physicochemical Properties of Non-Woven Polylactic Acid Fiber Materials

et al. 2021

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Biocompatible glycero (9,10-trioxolane) trioleate (ozonide of oleic acid triglyceride, OTOA) was incorporated into polylactic acid (PLA) fibers by electrospinning and nonwoven PLA mats with 1%, 3% and 5% OTOA content. The morphological, mechanical, thermal and water sorption properties of electrospun PLA mats after the addition of OTOA were studied. A morphological analysis showed that the addition of OTOA increased the average fiber diameter and induced the formation of pores on the fiber surface, leading to an increase in the specific surface area for OTOA-modified PLA fibrous mats. PLA fiber mats with 3% OTOA content were characterized by a highly porous surface morphology, an increased specific surface area and high-water sorption. Differential scanning calorimetry (DSC) was used to analyze the thermal properties of the fibrous PLA mats. The glass transition temperatures of the fibers from the PLA–OTOA composites decreased as the OTOA content increased, which was attributed to the plasticizing effect of OTOA. DSC results showed that OTOA aided the PLA amorphization process, thus reducing the crystallinity of the obtained nonwoven PLA–OTOA materials. An analysis of the mechanical properties showed that the tensile strength of electrospun PLA mats was improved by the addition of OTOA. Additionally, fibrous PLA mats with 3% OTOA content showed increased elasticity compared to the pristine PLA material. The obtained porous PLA electrospun fibers with the optimal 3% OTOA content have the potential for various biomedical applications such as drug delivery and in tissue engineering.

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Section: Introductionmentioning

confidence: 99%

Effect of Glycero-(9,10-trioxolane)-trialeate on the Physicochemical Properties of Non-Woven Polylactic Acid Fiber Materials

et al. 2021

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“…The catalase activity in different solutions was assessed using a previously published protocol with minor modifications [ 27 ]. Catalase-containing solutions were diluted in sample buffer (25 mM sodium phosphate buffer, 1 mM EDTA, 0.1% w/v bovine serum albumin, pH 7.5) to a catalase concentration of 5–10 μg/mL.…”

Section: Methodsmentioning

confidence: 99%

“…Furthermore, catalase is employed in photodynamic therapy (PDT), where excitation of a photosensitiser generates radical oxygen species which subsequently lead to tissue destruction [ 26 ]. As catalase generates oxygen by decomposition of H 2 O 2 it can combat the hypoxic microenvironment often found in solid tumors, which is a limiting factor in PDT [ 27 ]. Electrosprayed catalase formulations could thus be beneficial as a basis for therapeutics for a range of conditions.…”

Section: Introductionmentioning

confidence: 99%

Stable Dried Catalase Particles Prepared by Electrospraying

2022

Self Cite

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Therapeutic proteins and peptides are clinically important, offering potency while reducing the potential for off-target effects. Research interest in developing therapeutic polypeptides has grown significantly during the last four decades. However, despite the growing research effort, maintaining the stability of polypeptides throughout their life cycle remains a challenge. Electrohydrodynamic (EHD) techniques have been widely explored for encapsulation and delivery of many biopharmaceuticals. In this work, we explored monoaxial electrospraying for encapsulation of bovine liver catalase, investigating the effects of the different components of the electrospraying solution on the integrity and bioactivity of the enzyme. The catalase was successfully encapsulated within polymeric particles made of polyvinylpyrrolidone (PVP), dextran, and polysucrose. The polysorbate 20 content within the electrospraying solution (50 mM citrate buffer, pH 5.4) affected the catalase loading—increasing the polysorbate 20 concentration to 500 μg/mL resulted in full protein encapsulation but did not prevent loss in activity. The addition of ethanol (20% v/v) to a fully aqueous solution improves the electrospraying process by reducing surface tension, without loss of catalase activity. The polymer type was shown to have the greatest impact on preserving catalase activity within the electrosprayed particles. When PVP was the carrier there was no loss in activity compared with fresh aqueous solutions of catalase. The optimum particles were obtained from a 20% w/v PVP or 30% w/v PVP-trehalose (1:1 w/w) solution. The addition of trehalose confers stability advantages to the catalase particles. When trehalose-PVP particles were stored at 5 °C, enzymatic activity was maintained over 3 months, whereas for the PVP-only analogue a 50% reduction in activity was seen. This demonstrates that processing catalase by monoaxial electrospraying can, under optimised conditions, result in stable polymeric particles with no loss of activity.

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“…Dziemidowicz et al [104] reported post-fabrication functionalization of electrospun PCL fibers using photo-assisted perfluorophenyl azide-N-hydroxysuccinimide (NHS) PFPA-NHSA (solution of PFPA-NHS (20 mg/mL in methanol, 10 µL) was pipetted onto the surface of a PCL fiber mat (0.5 cm × 0.5 cm)). Afterwards, the fiber mats were placed under a UV lamp (8 W, 254 nm, 50 Hz) and irradiated from a distance of 10 cm.…”

Section: Curing Effectmentioning

confidence: 99%

Surface Functionalities of Polymers for Biomaterial Applications

Drobotă¹,

Ursache²,

Aflori³

2022

Polymers

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Changes of a material biointerface allow for specialized cell signaling and diverse biological responses. Biomaterials incorporating immobilized bioactive ligands have been widely introduced and used for tissue engineering and regenerative medicine applications in order to develop biomaterials with improved functionality. Furthermore, a variety of physical and chemical techniques have been utilized to improve biomaterial functionality, particularly at the material interface. At the interface level, the interactions between materials and cells are described. The importance of surface features in cell function is then examined, with new strategies for surface modification being highlighted in detail.

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A simple route to functionalising electrospun polymer scaffolds with surface biomolecules

Cited by 9 publications

References 31 publications

Effect of Glycero-(9,10-trioxolane)-trialeate on the Physicochemical Properties of Non-Woven Polylactic Acid Fiber Materials

Effect of Glycero-(9,10-trioxolane)-trialeate on the Physicochemical Properties of Non-Woven Polylactic Acid Fiber Materials

Stable Dried Catalase Particles Prepared by Electrospraying

Surface Functionalities of Polymers for Biomaterial Applications

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