A simple, rapid and sensitive fluorimetric assay for the measurement of cell-mediated cytotoxicity

The roots of Salvia miltiorrhiza (Danshen) and Pueraria lobata (Gegen) inhibit atherogenic events: A study of the combination effects of the 2-herb formula

“…1D), respectively. The cytotoxic effect of DE, GE and DGE on RAW 264.7 cells was assessed using 4-methylumbelliferyl heptanoate (MUH) assay as described by Virag et al (1995). The non-cytotoxic concentration was observed in the range of 0-2 mg/ml (data not shown), which confirmed that the observed effects of DE, GE and DGE were not due to their cytotoxicity.…”

Extracts of Lindera obtusiloba induce antifibrotic effects in hepatic stellate cells via suppression of a TGF-β-mediated profibrotic gene expression pattern

“…Cell morphology was assessed by light microscopy (Olympus, Hamburg, Germany) after sulforhodamine B staining as previously described [21]. Cell viability/cytotoxicity was determined by conversion of 4-methylumbelliferyl heptanoate (Sigma, Deisenhofen, Germany) to fluorescent 4-methylumbelliferone (λ ex : 355 nm, λ em : 460 nm) [22]. Fluorescence was determined using a SpectraMax Gemini EM 96-well microplate fluorometer (Molecular Devices, Sunnyvale, CA) and 96-well black microplates (Greiner Bio-One, Frickenhausen, Germany).…”

The increased expression of Y box‐binding protein 1 in melanoma stimulates proliferation and tumor invasion, antagonizes apoptosis and enhances chemoresistance

“…The intensity of fluorescence indicates the number of vital cells in the wells. 26,27 For the analysis of chemosensitivity, cells were incubated 24 hr after plating with cisplatin, etoposide or doxorubicin at the indicated concentrations and 72 hr later the assay using MUH as a substrate was performed as described above. The percentage of living cells was calculated compared to the respective nontreated control cells.…”