A Simple, Rapid 125I Radioimmunoassay for the Detection of Barbiturates in Biological Fluids

Abstract

A radioimmunoassay using a 3H- or 125I-labeled morphine antigen has been shown to be of practical value for the rapid detection of nanogram amounts of morphine and morphine analogs in biological fluids [1–3]. A radioimmunoassay for detection of barbiturates by conjugation of a secobarbital derivative to protein was also developed utilizing 14C-labeled pentobarbital or barbital [4,5]. The present report describes our experiences with a practical radioimmunoassay for barbiturates employing a secobarbital derivative labeled with 125I, as the antigen.

Radioimmunological determination of cocaine in human hair

“…Determination of drugs in body fluids, tissues, stains, and hair by radioimmunoassays has been documented (Spector and Parker 1970;Spector and Flynn 1971;Catlin et al 1973;Cheng et al 1973;Berman et al 1975;Cleeland et al 1975;Mul6 et al 1975;Shaler et al 1978;Baumgartner et al 1979Baumgartner et al , 1981Smith 1981;Smith and Pomposini 1981;Valente et al 1981). The sensitivity of this method makes it possible to detect the agent a long time after drug abuse or administration without pretreatment of the samples.…”

Tunable Detection Sensitivity of Opiates in Urine via a Label-Free Porous Silicon Competitive Inhibition Immunosensor

“…The label may be a radioisotope,3 fluorophore,4 or most commonly an enzyme that produces changes in light absorbance 5,6. Label-free sensor technologies, such as surface plasmon resonance (SPR), are also currently being investigated for DOA detection 7.…”