Radioimmunological determination of cocaine in human hair

Abstract

Summary.A simple procedure for the determination of cocaine in human hair was described. After washing hair samples were crushed in 0.1 m HC1 and incubated overnight at 45°C. The acid extracts were neutralized with 1 m NaOH. Phosphate buffer (pH 7.4) was added to the extracts. The cocaine concentrations were measured by radioimmunoassay. Detection in hair was achieved in all hair samples obtained from cocaine users. This method appears to be suitable for the routine determination of cocaine. Key word: Cocaine, radioimmunological determination in hairZusammenfassung. Diese Arbeit beschreibt eine Methode fiir die Bestimmung von Kokain im menschlichen Haar. Das Haar wurde gewaschen, zermalt mit 0,1m HC1 und tiber Nacht bei 45°C inkubiert. Nach Neutralisierung mit 1 m NaOH wurde Phosphat-Puffer (pH 7,4) zugesetzt. Die KokainKonzentrationen wurden mittels Radioimmunoassay bestimmt. In allen Haarproben yon Kokainabh~ingigen wurden megbare Konzentrationen nachgewiesen. Dieses Veffahren stellt eine schnelle und einfache Routinemethode dar.

Interaction of Minoxidil with Pigment in Cells of the Hair Follicle: An Example of Binding without Apparent Biological Effects

“…There are many reports claiming success in measur ing a variety of drugs including amphet amines, cocaine, haloperidol, methadone, ni cotine, opiates, and phencyclidine in human hair [11][12][13][14][15][16]. With haloperidol, the measured concentrations were higher in pigmented hair than in nonpigmented hair for both humans and rats treated with the drug [17], This and our minoxidil data definitely link hair color with differential uptake of drugs.…”

Determination of cocaine in human hair by gas chromatography/mass spectrometry

“…Determination of cocaine concentrations in hair by radioimmunoassay has been reported (Valente et al 1981;Baumgartner et al 1982;Smith and Liu 1986;Balabanova et al 1987). The antibody used in the assay is benzoylecgonine antiserum which reacts with benzoylecgonine, but also with cocaine, ecgonine, and ecgonine methyl ester.…”

Hair analysis for veterinary drug monitoring in livestock production

“…Other substances have been reported as being accumulated in equine hair such as procaine, benzilpenicilin, trimetoprim, sulfadiazine, metronidazol [17] or cocaine in sheep's wool [68].…”