A simple purification and activity assay of the coagulant protein from Moringa oleifera seed

Ghebremichael, Kebreab; Gunaratna, K.R.; Henriksson, Hongbin; Brumer, Harry; Dalhammar, Gunnel

doi:10.1016/j.watres.2005.04.012

Cited by 337 publications

(280 citation statements)

References 15 publications

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“…MO were cationic proteins with a molecular mass of 6.5-13 kDa and isoelectric points in the range of pH 9.6-11 (Ghebremichael et al, 2005). It was chosen as the ZP adjuster for M.A.…”

Section: Mo Chitosan Soil and Modification Processmentioning

confidence: 99%

“…Besides MO, there are some other widely existing proteins with a high isoelectric point which possess a net positive charge in natural waters and show promise to achieve this goal. Ghebremichael et al (2005) have proved that there are many other small, basic peptides from plants and animals which can be used to reduce the surface charge of particles. Therefore, a new bi-component modification method using locally available, biodegradable and nontoxic materials with high charge density and chitosan could be developed, which combines multi mechanism of charge neutralization to manipulate the ZP and netting and bridging function to improve the floc size.…”

Section: Environmental Implicationsmentioning

confidence: 99%

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Influence of zeta potential on the flocculation of cyanobacteria cells using chitosan modified soil

Zhang

Pan

2015

Journal of Environmental Sciences

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Using chitosan modified soil to flocculate and sediment algal cells has been considered as a promising strategy to combat cyanobacteria blooms in natural waters. However, the flocculation efficiency often varies with algal cells with different zeta potential (ZP) attributed to different growth phases or water conditions. This article investigated the relationship between ZP of Microcystis aeruginosa and its influence to the flocculation efficiency using chitosan modified soil. Results suggested that the optimal removal efficiency was obtained when the ZP was between − 20.7 and − 6.7 mV with a removal efficiency of more than 80% in 30 min and large floc size of > 350 μm. When the algal cells were more negatively charged than − 20.7 mV, the effect of chitosan modified soil was depressed (< 60%) due to the insufficient charge density of chitosan to neutralize and destabilize the algal suspension. When the algal cells were less negative than − 6.7 mV or even positively charged, a small floc size (< 120 μm) was formed, which may be difficult to sink under natural water conditions. Therefore, manipulation of ZP provided a viable tool to improve the flocculation efficiency of chitosan modified soil and an important guidance for practical engineering of cyanobacteria bloom control.

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“…MO were cationic proteins with a molecular mass of 6.5-13 kDa and isoelectric points in the range of pH 9.6-11 (Ghebremichael et al, 2005). It was chosen as the ZP adjuster for M.A.…”

Section: Mo Chitosan Soil and Modification Processmentioning

confidence: 99%

Section: Environmental Implicationsmentioning

confidence: 99%

Influence of zeta potential on the flocculation of cyanobacteria cells using chitosan modified soil

Zhang

Pan

2015

Journal of Environmental Sciences

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“…The dry pods are adequate to use as a substratum for laboratory animal bedding 9 . The seeds show antimicrobial activity against fungi 10,11 and bacteria 10,12,13 , antitumor 14 , anti-inflammatory, antispasmodic, diuretic 15 and larvicidal activity against the mosquito that transmits dengue and yellow fever 16 . Gupta et al 17 showed that the roots were able to depress the central nervous system, cause analgesia and potentiate the analgesic effect of morphine.…”

Section: Moringa Oleifera Lamarck (Synonymmentioning

confidence: 99%

“…The aqueous seed extract has been traditionally used to purify water in Africa and, in South Asian countries, as a natural coagulant since it has high levels of active cationic proteins with molecular mass between 6 and 16kDa and highly alkaline isoelectric points 3,13,46,47 , with a coagulation efficiency similar to that of alumen in samples with high turbidimetry 13 . Studies have demonstrated low toxicity of this water extract, with a LD 50 value of 512.8mg/kg body weight 16,48,49 .…”

Section: Nutritional Potentialmentioning

confidence: 99%

Moringa oleifera: bioactive compounds and nutritional potential

et al. 2008

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This work aims to review the nutritional properties of the Moringa oleifera tree, emphasizing its main constituents and nutritional applications for humans and animals. Moringa oleifera (Moringaceae) is a cosmopolitan tree that grows in many tropical countries showing uncountable folk uses due to its various nutritional and pharmacological applications. The young leaves, flowers and pods are common vegetables in the Asian diet. All parts of this plant are renewable sources of tocopherols (γ and α), phenolic compounds, ß-carotene, vitamin C and total proteins, including the essential sulfur amino acids, methionine and cysteine. The seed protein and fat contents are higher than those reported for important grain legumes and soybean varieties, respectively. Unsaturated fatty acids, especially oleic acid, carbohydrates and minerals are present in the seed in reasonable amounts. In general, there are low concentrations of antinutritional factors in the plant, although the seeds possess glucosinolates (65.5µmol/g dry matter), phytates (41g/kg) and hemagglutination activity while the leaves have appreciable amounts of saponins (80g/kg), besides low quantity of phytates (21g/kg) and tannins (12g/kg). Taking into consideration the excellent nutritional properties, the low toxicity of the seeds and the excellent ability of the plant to adapt to poor soils and dry climates, Moringa oleifera can be an alternative to some leguminous seeds as a source of high-quality protein, oil and antioxidant compounds and a way to treat water in rural areas where appropriate water resources are not available.Indexing terms: Protease Inhibitors. Moringa oleifera. Nutritive value. R E S U M OO objetivo deste trabalho é fazer uma revisão sobre as propriedades nutricionais da planta Moringa oleifera, enfatizando seus principais constituintes e suas aplicações nutricionais para o homem e os animais. Moringa

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“…The moringa plant (Moringa oleifera) has been the object of much research due to its multiple uses and well-known bactericidal potential 9,37,17,38 . According to BEZERRA et al 8 , the moringa tree is native to northeastern India.…”

Section: Introductionmentioning

confidence: 99%

Antibacterial effect (in vitro) of Moringa oleifera and Annona muricata against Gram positive and Gram negative bacteria

Viera

Mourão

Ângelo

et al. 2010

Rev. Inst. Med. trop. S. Paulo

135

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SUMMARYAntibacterial effects of aqueous and ethanolic extracts of seeds of moringa (Moringa oleifera) and pods of soursop (Annona muricata) in the concentration of 1:5 and 1:10 in volumes 50, 100, 150 and 200 μL were examined against Staphylococcus aureus, Vibrio cholerae, Escherichia coli (isolated from the organism and the aquatic environment) and Salmonella Enteritidis. Antibacterial activity (inhibition halo > 13 mm) against S. aureus, V. cholerae and E. coli isolated from the whiteleg shrimp, Litopenaeus vannmaei, was detected in aqueous and ethanolic extracts of moringa. E. coli isolated from tilapiafish, Oreochromis niloticus, was sensitive to the ethanolic extract of moringa. The aqueous extracts of soursop showed an antibacterial effect against S. aureus and V. cholerae, but the antibacterial activity by the ethanol extracts of this plant was not demonstrated.

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A simple purification and activity assay of the coagulant protein from Moringa oleifera seed

Cited by 337 publications

References 15 publications

Influence of zeta potential on the flocculation of cyanobacteria cells using chitosan modified soil

Influence of zeta potential on the flocculation of cyanobacteria cells using chitosan modified soil

Moringa oleifera: bioactive compounds and nutritional potential

Antibacterial effect (in vitro) of Moringa oleifera and Annona muricata against Gram positive and Gram negative bacteria

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