A simple procedure for estimation of total body surface area and determination of a new value of Meeh's constant in rats

Gouma, Efthalia; Simos, Yannis V.; Verginadis, Ioannis I.; Lykoudis, Efstathios G.; Evangelou, Angelos; Karkabounas, Spyridon

doi:10.1258/la.2011.011021

Cited by 111 publications

(74 citation statements)

References 21 publications

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“…There was no significant difference in the TBSA between these groups (p>0.05). TBSAs were calculated according to Meeh’s formula (TBSA= kW 2/3 ) with a k constant of 9.83 [30]. …”

Section: Resultsmentioning

confidence: 99%

Topically applied metal chelator reduces thermal injury progression in a rat model of brass comb burn

Wang

Ayadi

Goswamy

et al. 2015

Burns

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Oxidative stress may be involved in the cellular damage and tissue destruction as burn wounds continues to progress after abatement of the initial insult. Since iron and calcium ions play key roles in oxidative stress, this study tested whether topical application of Livionex formulation (LF) lotion, that contains disodium EDTA as a metal chelator and methyl sulfonyl methane (MSM) as a permeability enhancer, would prevent or reduce burn injury. Methods We used an established brass comb burn model with some modifications. Topical application of LF lotion was started 5 minutes post-burn, and repeated every 8 hours for 3 consecutive days. Rats were euthanized and skin harvested for histochemistry and immunohistochemistry. Formation of protein adducts of 4-hydroxynonenal (HNE), malonadialdehyde (MDA) and acrolein (ACR) and expression of aldehyde dehydrogenase (ALDH) isozymes, ALDH1 and ALDH2 were assessed. Results LF lotion-treated burn sites and interspaces showed mild morphological improvement compared to untreated burn sites. Furthermore, the lotion significantly decreased the immunostaining of lipid aldehyde-protein adducts including protein -HNE, -MDA and -ACR adducts, and restored the expression of aldehyde dehydrogenase isozymes in the unburned interspaces. Conclusion This data, for the first time, demonstrates that a topically applied EDTA-containing lotion protects burn injury progression with a concomitant decrease in the accumulation of reactive lipid aldehydes and protection of aldehyde dehydrogenase isozymes. Present studies are suggestive of therapeutic intervention of burn injury by this novel lotion.

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“…There was no significant difference in the TBSA between these groups (p>0.05). TBSAs were calculated according to Meeh’s formula (TBSA= kW 2/3 ) with a k constant of 9.83 [30]. …”

Section: Resultsmentioning

confidence: 99%

Topically applied metal chelator reduces thermal injury progression in a rat model of brass comb burn

Wang

Ayadi

Goswamy

et al. 2015

Burns

View full text Add to dashboard Cite

show abstract

“…For the calculation of the body surface area we used “Meeh’s formula”, which reads as follows: A = K × W 2/3 . Hereby, A represents the body surface area, K an animal specific constant, which was set to 9.83 and W the individual body weight [8]. …”

Section: Methodsmentioning

confidence: 99%

Liver-directed chemotherapy of cetuximab and bevacizumab in combination with oxaliplatin is more effective to inhibit tumor growth of CC531 colorectal rat liver metastases than systemic chemotherapy

Sperling

Brandhorst

Schäfer

et al. 2012

Clin Exp Metastasis

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Colorectal carcinoma is, through to its high rate of liver metastasis (mCRC), the second most cause of cancer death worldwide. Tumor resection represents the only potential cure. In cases of unresectable disease systemic chemotherapy (sCHT) remains the therapy of choice. Modern sCHT regimens including biological agents can induce tumor response that leads to curative surgery of initially unresectable mCRC. However, liver-directed therapy via hepatic arterial infusion (HAI) may produce higher response rates than sCHT. Herein we studied whether a HAI of cetuximab (CE) plus bevacizumab (BE) with or without oxaliplatin (OX) can inhibit tumor growth in a rat model. WAG/Rij rats underwent subcapsular hepatic tumor implantation. After 10 days animals received either HAI or sCHT of CE plus BE, OX or all three drugs. Saline-treated animals served as controls. Tumor growth was estimated at day 10 and 13. On day 13 liver and tumor tissue was studied histologically and immunohistochemically. In controls the tumors grew about 50 %. OX alone was not capable of inhibiting tumor growth. In contrast, CE plus BE given as HAI significantly reduced tumor growth compared to sCHT (p < 0.05). HAI of CE plus BE combined with OX yielded an even more pronounced inhibition of tumor growth. Immunohistochemistry revealed a decreased tumor cell proliferation and tumor vascularization. The present study demonstrates that HAI of CE plus BE is effective to inhibit tumor growth. This effect is even more pronounced in combination with OX. Systemic application of these agents cannot achieve comparable effects.

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“…One study reported the weekly food consumption for each experimental group, allowing the calculation of an average daily intake of 212 mg of DHA in the ‘Excess DHA’ group (Church, Jen, 2007). In an average-weight lactating rat, assuming a total body surface area (TBSA) of 390 cm 2 using Meeh’s formula (Gouma et al, 2012, Hanson et al, 2001) the “Excess DHA” group’s dose corresponds to approximately 5.4 grams per m 2 . For an adult human the equivalent dose is just over 11 grams of DHA daily (Verbraecken et al, 2006).…”

Section: Resultsmentioning

confidence: 99%

Neurophysiologic measures of auditory function in fish consumers: Associations with long chain polyunsaturated fatty acids and methylmercury

Dziorny¹,

Orlando²,

Strain³

et al. 2013

NeuroToxicology

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Background Determining if associations exist between child neurodevelopment and environmental exposures, especially low level or background ones, is challenging and dependent upon being able to measure specific and sensitive endpoints. Psychometric or behavioral measures of CNS function have traditionally been used in such studies, but do have some limitations. Auditory neurophysiologic measures examine different nervous system structures and mechanisms, have fewer limitations, can more easily by quantified, and might be helpful testing additions. To date, their use in human epidemiological studies has been limited. We reviewed the use of auditory brainstem responses (ABR) and otoacoustic emissions (OAE) in studies designed to determine the relationship of exposures to methyl mercury (MeHg) and nutrients from fish consumption with neurological development. We included studies of experimental animals and humans in an effort to better understand the possible benefits and risks of fish consumption. Objectives We reviewed the literature on the use of ABR and OAE to measure associations with environmental exposures that result from consuming a diet high in fish. We focused specifically on long chain polyunsaturated fatty acids (LCPUFA) and MeHg. Methods We performed a comprehensive review of relevant studies using web-based search tools and appropriate search terms. Results Gestational exposure to both LCPUFA and MeHg has been reported to influence the developing auditory system. In experimental studies supplemental LCPUFA is reported to prolong ABR latencies and human studies also suggest an association. Experimental studies of acute and gestational MeHg exposure are reported to prolong ABR latencies and impair hair cell function. In humans, MeHg exposure is reported to prolong ABR latencies, but the impact on hair cell function is unknown. Conclusion The auditory system can provide objective measures and may be useful in studying exposures to nutrients and toxicants and whether they are associated with children’s neurodevelopment.

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A simple procedure for estimation of total body surface area and determination of a new value of Meeh's constant in rats

Cited by 111 publications

References 21 publications

Topically applied metal chelator reduces thermal injury progression in a rat model of brass comb burn

Topically applied metal chelator reduces thermal injury progression in a rat model of brass comb burn

Liver-directed chemotherapy of cetuximab and bevacizumab in combination with oxaliplatin is more effective to inhibit tumor growth of CC531 colorectal rat liver metastases than systemic chemotherapy

Neurophysiologic measures of auditory function in fish consumers: Associations with long chain polyunsaturated fatty acids and methylmercury

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