A simple pneumatic setup for driving microfluidics

Braschler, Thomas; Metref, Lynda; Zvitov–Marabi, Ronit; Lintel, Harald van; Demierre, Nicolas; Theytaz, Joël; Renaud, Philippe

doi:10.1039/b617673a

Cited by 32 publications

(27 citation statements)

References 6 publications

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“…S1) [24] that ensured tight sealing and good control over flow rates in the microfluidic gradient maker. After priming the microfluidic device under vacuum, up to 15 mL of protein solutions and buffer were loaded into the inlets to equal height, and the system was pressurized (50 mbar, 1 bar ¼ 10 3 kPa) to establish steady-state gradients.…”

Section: Methodsmentioning

confidence: 99%

“…2a, Step 1) using a pneumatic set-up. [24] This approach turned out to be sufficient to ensure tight sealing. Because of the differences in dimensions between the channels (depth: 100 mm) and gel films (thickness: 25 mm), the hydrogels filled only a relatively small portion of the www.afm-journal.de Step 1: The microfluidic chip is pressed onto the functionalized hydrogel cast on a silanized glass slide.…”

Section: Microfluidic Patterning Of Protein Gradients On Peg Hydrogelmentioning

confidence: 99%

See 1 more Smart Citation

Capturing Complex Protein Gradients on Biomimetic Hydrogels for Cell‐Based Assays

Cosson¹,

Köbel²,

Lütolf³

2009

Adv Funct Materials

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A versatile strategy to rapidly immobilize complex gradients of virtually any desired protein on soft poly(ethylene glycol) (PEG) hydrogel surfaces that are reminiscent of natural extracellular matrices (ECM) is reported. A microfluidic chip is used to generate steady‐state gradients of biotinylated or Fc‐tagged fusion proteins that are captured and bound to the surface in less than 5 min by NeutrAvidin or ProteinA, displayed on the surface. The selectivity and orthogonality of the binding schemes enables the formation of parallel and orthogonal overlapping gradients of multiple proteins, which is not possible on conventional cell culture substrates. After patterning, the hydrogels are released from the microfluidic chip and used for cell culture. This novel platform is validated by conducting single‐cell migration experiments using time‐lapse microscopy. The orientation of cell migration, as well as the migration rate of primary human fibroblasts, depends on the concentration of an immobilized fibronectin fragment. This technique can be readily applied to other proteins to address a wealth of biological questions with different cell types.

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Section: Methodsmentioning

confidence: 99%

Section: Microfluidic Patterning Of Protein Gradients On Peg Hydrogelmentioning

confidence: 99%

Capturing Complex Protein Gradients on Biomimetic Hydrogels for Cell‐Based Assays

Cosson¹,

Köbel²,

Lütolf³

2009

Adv Funct Materials

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show abstract

“…In this experiment, 0.1% (w/v) polystyrene particles (490 nm in diameter, Spherotech inc. IL, USA) solution were used; high pressure nitrogen was downregulated as a driving force for the fluid flow in the microchannel [25] ; the driving pressure was 1 kPa; flow phenomena was observed by 100 X oil objective lens and their imaging were captured at 15 f/s. The continuous images (Figure 9(a)) were analyzed by the program of our laboratory written in MATLAB platform.…”

Section: Articlesmentioning

confidence: 99%

PDMS microchannel fabrication technique based on microwire-molding

Jia

Jiang

et al. 2008

Sci. Bull.

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“…5,6 Syringe pumps are one of the simplest and most widely used tools, but inevitably, problems arise due to oscillations in flow rate, limited syringe volume, and inability to tightly control flow at extremely low flow rates (i.e., <1 μl min −1 ). 7 Electroosmotic flow has been demonstrated to provide smooth flow at low flow rates and is scalable to chip-sized devices, although the complexity and necessity of an electrolyte limits its applicability.…”

mentioning

confidence: 99%