PDMS microchannel fabrication technique based on microwire-molding

Jia, YueFei; Jiang, Jiahuan; Ma, Xiaodong; Li, Yuan; Huang, Heming; Kunbao, Cai; Cai, Shaoxi; Yunpeng, Wu

doi:10.1007/s11434-008-0528-6

Cited by 51 publications

(47 citation statements)

References 25 publications

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“…The image on the right-hand side shows the two orthogonal microchannels formed after removing two corresponding cross-contacted stainless steel microwires; the through-hole formed at the cross-site is marked by an arrow these cases was estimated to be about 5 pL/s given a pressure difference of 1.5 kPa. As characterized previously, the microflow behaviour in the microchannel under such conditions would be laminar [15]. Figure 2 shows the results of the adsorption of ssDNA (HS-(C6)-CCTGAATAGCATCTTGAC-(C6)-FAM) (dissolved in 0.25 mol/L NaCl, pH 7.0) onto microdots (that is, stainless steel microelectrodes) in a microchannel when a d.c. voltage of 1.6 V was applied across a pair of microdot electrodes in one microchannel.…”

Section: Experimental Methodsmentioning

confidence: 80%

In situDNA immobilization on stainless steel microelectrodes in a microchannel

Jiang

et al. 2008

Proceedings of the Institution of Mechanical Engineers, Part N:

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DNA immobilization at specific locations on the inner wall of microchannels could lead to many interesting applications. In this paper, three procedures (electrostatic adsorption, thiol-Au covalent bonding, and electrochemical entrapment) were carried out for the immobilization of DNA probes onto stainless steel microdot electrodes inside a polydimethylsiloxane (PDMS) microchannel. The microdot electrodes were fabricated via crossed microwire moulding, a modified soft lithographic technique, to be naked on the inner wall of the PDMS microchannel. The immobilization of DNA probes onto the surface of the microdot electrodes was performed by microfluidics. It was found that, for DNA electrostatic adsorption, different electrode behaviours were exhibited inside and outside the microchannel; in the microchannel, the negatively charged DNA probes would undergo abnormal electrostatic adsorption onto the cathode. Furthermore, DNA immobilization through thiol-Au covalent bonding and DNA entrapment in enhanced polypyrrole (PPy)-modified dots inside microchannels was demonstrated. Further work is needed, but present results are promising and suggest a route by which microfluidics may be married with label-free electrochemical detection and with very low-cost DNA diagnostics, which could prove invaluable.

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Section: Experimental Methodsmentioning

confidence: 80%

In situDNA immobilization on stainless steel microelectrodes in a microchannel

Jiang

et al. 2008

Proceedings of the Institution of Mechanical Engineers, Part N:

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show abstract

“…Fabrication of complex geometries as reported in (Song et al, 2010;Jia et al, 2008) was possible using our fabrication method. 3D circular microfluidic channel of diameter 150 m with the length of 1 cm was fabricated for development of immuno-biosensor device.…”

Section: Resultsmentioning

confidence: 99%

“…1d) and immersed in ethanol for 30 min. PDMS swelling in ethanol (swelling coefficient 1.04) (Jia et al, 2008) facilitated easy removal of wires. The wires were drawn out manually by applying a gentle force leading to formation of circular microchannels in PDMS as indicated by arrows in Fig.…”

Section: Introductionmentioning

confidence: 99%

Quantum dot based immunosensor using 3D circular microchannels fabricated in PDMS

Morarka

Agrawal

Kale

et al. 2011

Biosensors and Bioelectronics

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“…8 Such channels, which are topologically equivalent to planar channels, are generated by bending planar channels in PDMS. Another method is to use a 3D template, such as threads, 9 wires 10,11 soldering wires 12 and soluble organic materials. The templates can be removed by pulling threads or wires out of the PDMS, melting soldering wires or dissolving organic materials.…”

Section: Introductionmentioning

confidence: 99%