A simple PCR-based method for scoring the ph1b deletion in wheat

Qu, Li‐Jia; Foote, Tracie; Roberts, Michael; Money, Tracy; Aragón-Alcaide, Luis; Snape, J. W.; Moore, Graham

doi:10.1007/s001220050751

Cited by 52 publications

(27 citation statements)

References 10 publications

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“…The converted SCARs are highly reliable, relatively inexpensive, and can be easily manipulated. Thus, they are valuable in practical breeding programs where large numbers of individuals need to be genotyped at low cost (Kelly et al, 2003), and for map-based gene cloning (Bradeen and Simon, 1998;Qu et al, 1998;). In the literature, there are examples of converting AFLP markers into SCAR markers (Shan et al, 1999;Boukar et al, 2004;Shirasawa et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

Identification of an AFLP marker linked with yellow rust resistance in wheat (Triticum aestivum L.)

BALTA¹,

Metin²,

Akfirat³

et al. 2014

Turk J Biol

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An important application of molecular markers in plant systems involves improvement in the efficiency of conventional plant breeding by carrying out indirect selection through molecular markers linked to the traits of interest. AFLP analysis was used to identify molecular markers associated with yellow rust resistance in wheat (Triticum aestivum L.) in this study. DNA isolated from the selected yellow rust tolerant and susceptible F 2 individuals derived from a cross between Izgi2001 (resistant) and ES14 (susceptible) at seedling and adult stage was used for bulked segregant analysis combined with AFLP. From the screening of 34 PstI/MseI AFLP primer combinations, the AFLP marker P-GAC/M-ACG (133 bp) was identified and presented in the resistant parent and resistant F 2 individuals but not in the susceptible ones. Future research will obtain more adjacent sequences associated with the polymorphic M-ACG/P-GAC 133bp marker by the PCR walking method to design SCAR markers for wheat breeding programs.

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Section: Discussionmentioning

confidence: 99%

Identification of an AFLP marker linked with yellow rust resistance in wheat (Triticum aestivum L.)

BALTA¹,

Metin²,

Akfirat³

et al. 2014

Turk J Biol

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“…Haploids were produced on this Phph heterozygote product and haploids with the ph locus were detected by PCR (Qu et al 1998), doubled with colchicine, selfed a few times with the progeny then becoming a source of homoeologous and/or nonhomoeologous translocations between ABD and its three alien inclusions i.e. 3,4 and 7E b that await analyses (Mujeeb-Kazi 2003).…”

Section: Production Of New Translocationsmentioning

confidence: 99%

Tissue Culture Mediated Allelic Diversification and Genomic Enrichment of Wheat to Combat Production Constraints and Address Food Security

Kazi

Ali

Ibrahim

et al. 2017

Plant Tissue Cult. & Biotech.

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In view of the emerging population trends and that wheat crop is the major unequivocally recognized conduit towards addressing the food security challenges of 2050 this discourse embraces various research options that are viewed as possible solutions toward delivering those targets for providing nutritious food and meeting the aspirations that policy setters have debated on the subject for decades. The underlying strength for achieving these targets will require concerted efforts from plant researchers that are well integrated within effectively harnessing and utilizing prevalent genetic diversity of the wide array of alleles in a holistic pro-active manner. We argue that the purists of basic and strategic research dimensions need to be thoughtfully defined, so that the vital target of delivering the "applied" gains are only realized from the outputs on farmer's fields and measured by tons per hectare. In this quest, the pre-breeding disciplines "classical mode" and its recently surfaced "modified sense" are pivotal, where within the former facet "tissue culture" (TC)/artificial culturing is embodied integrally. Taken for granted, TC has been the backbone of all wide hybridization studies and has made an enormous impact on the agricultural landscape spanning over the last six decades. With its intervention significant generic and specific incompatibilities have been overcome as well as allowing

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“…In the present study, the inverse PCR approach was carried out to obtain DNA sequences adjacent to the AFLP marker. Inverse PCR was chosen because of its simplicity and the simultaneous use of the two specific internal primers (Ochman et al 1988;Brigneti et al 1997;De Jong et al 1997;Bradeen and Simon 1998;Qu et al 1998) instead of genome walking (Negi et al 2000;Brugmans et al 2003).…”

Section: Inverse Pcrmentioning

confidence: 99%

Development of a diagnostic DNA marker for the geographic origin of Shorea leprosula

2016

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Abstract:The development of sequence characterized amplified region (SCAR) markers derived from amplified fragment length polymorphisms (AFLPs) is described for Shorea leprosula. An AFLP fragment that showed nearly complete differentiation between Borneo and Sumatra was gel-extracted, sequenced, and converted into a SCAR marker using the inverse polymerase chain reaction (PCR) technique. The single nucleotide polymorphism (SNP) that originally caused the AFLP was found in the MseI restriction site. Differentiation between islands was detected either as size variation of the codominant SCAR marker or after digestion of the PCR products with the restriction enzyme MseI (PCR-RFLP). Size variation was due to insertions/deletions found within the sequenced region that flanked the original AFLP fragment. After genotyping 151 samples of S. leprosula from 14 populations in Sumatra and Borneo, all but one sample from Sumatra were homozygous for one size variant (427 bp), while S. leprosula populations from Borneo showed different genotypes than Sumatra populations and variation not only among populations but also within populations. Complete differentiation and fixation on alternative variants was found for the geographic regions of Sumatra and Borneo by the PCR-RFLP method. The SCAR marker did not amplify in Shorea parvifolia and thus can also be used to distinguish between S. leprosula and S. parvifolia. The marker was successfully amplified from wood DNA extracts suggesting its applicability to track the geographic origin of timber.

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A simple PCR-based method for scoring the ph1b deletion in wheat

Cited by 52 publications

References 10 publications

Identification of an AFLP marker linked with yellow rust resistance in wheat (Triticum aestivum L.)

Identification of an AFLP marker linked with yellow rust resistance in wheat (Triticum aestivum L.)

Tissue Culture Mediated Allelic Diversification and Genomic Enrichment of Wheat to Combat Production Constraints and Address Food Security

Development of a diagnostic DNA marker for the geographic origin of Shorea leprosula

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