A simple pathway in preparation of controlled porosity of biphasic calcium phosphate scaffold for dentin regeneration

AbdulQader, Sarah Talib; Rahman, Ismail Ab; Ismail, Hanafi; Kannan, Thirumulu Ponnuraj; Mahmood, Zuliani

doi:10.1016/j.ceramint.2012.08.089

Cited by 35 publications

(13 citation statements)

References 33 publications

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“…Each run was performed with 2θ values between 20 and 40 at a step of 5°/min. The porosities of the scaffolds were measured by the liquid displacement method [ 24 ]. The density and porosity were evaluated according to Archimedes’ principle.…”

Section: Methodsmentioning

confidence: 99%

3D Printing Bioceramic Porous Scaffolds with Good Mechanical Property and Cell Affinity

et al. 2015

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Artificial bone grafting is widely used in current orthopedic surgery for bone defect problems. Unfortunately, surgeons remain unsatisfied with the current commercially available products. One of the major complaints is that these products cannot provide sufficient mechanical strength to support the human skeletal structure. In this study, we aimed to develop a bone scaffold with better mechanical property and good cell affinity by 3D printing (3DP) techniques. A self-developed 3D printer with laser-aided gelling (LAG) process was used to fabricate bioceramic scaffolds with inter-porous structures. To improve the mechanical property of the bioceramic parts after heating, CaCO3 was added to the silica ceramic slurry. CaCO3 was blended into a homogenous SiO2-sol dispersion at weight ratios varying from 0/100 to 5/95 to 9/91 (w/w). Bi-component CaCO3/SiO2-sol was prepared as a biocomposite for the 3DP scaffold. The well-mixed biocomposite was used to fabricate the bioceramic green part using the LAG method. The varied scaffolds were sintered at different temperatures ranging from 900 to 1500°C, and the mechanical property was subsequently analyzed. The scaffolds showed good property with the composite ratio of 5:95 CaCO3:SiO2 at a sintering temperature of 1300°C. The compressive strength was 47 MPa, and the porosity was 34%. The topography of the sintered 3DP bioceramic scaffold was examined by SEM, EDS and XRD. The silica bioceramic presented no cytotoxicity and good MG-63 osteoblast-like cell affinity, demonstrating good biocompatibility. Therefore, the new silica biocomposite is viable for fabricating 3DP bone bioceramics with improved mechanical property and good cell affinity.

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Section: Methodsmentioning

confidence: 99%

3D Printing Bioceramic Porous Scaffolds with Good Mechanical Property and Cell Affinity

et al. 2015

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“…BCP is a more effective ceramic for bone repair and regeneration than pure HAp or pure TCP alone because it controls the scaffold degradation rate. 23,24 Combining polymers with inorganic materials such as BCP particles can improve the bioactivity of electrospun scaffolds and enhance cell attachment, proliferation, and osteoblastic differentiation. [25][26][27] [BMPs], transforming growth factor-b, fibroblast growth factor, platelet-derived growth factor, and insulin-like growth factor), which are important signals in the osteogenic microenvironment.…”

mentioning

confidence: 99%

In VitroandIn VivoStudies of BMP-2-Loaded PCL–Gelatin–BCP Electrospun Scaffolds

Kim

Linh

Min

et al. 2014

Tissue Engineering Part A

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To confirm the effect of recombinant human bone morphogenetic protein-2 (BMP-2) for bone regeneration, BMP-2-loaded polycaprolactone (PCL)-gelatin (Gel)-biphasic calcium phosphate (BCP) fibrous scaffolds were fabricated using the electrospinning method. The electrospinning process to incorporate BCP nanoparticles into the PCL-Gel scaffolds yielded an extracellular matrix-like microstructure that was a hybrid system composed of nano-and micro-sized fibers. BMP-2 was homogeneously loaded on the PCL-Gel-BCP scaffolds for enhanced induction of bone growth. BMP-2 was initially released at high levels, and then showed sustained release behavior for 31 days. Compared with the PCL-Gel-BCP scaffold, the BMP-2-loaded PCL-Gel-BCP scaffold showed improved cell proliferation and cell adhesion behavior. Both scaffold types were implanted in rat skull defects for 4 and 8 weeks to evaluate the biological response under physiological conditions. Remarkable bone regeneration was observed in the BMP-2/PCL-Gel-BCP group. These results suggest that BMP-2-loaded PCL-Gel-BCP scaffolds should be considered for potential bone tissue engineering applications.

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“…This result is very similar with the results of previous work. 27 The scaffolds consisted of highly crystalline phases as indicated by the narrow and sharp diffraction peaks. This is in agreement with the works reported by earlier researchers.…”

Section: Discussionmentioning

confidence: 99%

“…Biphasic calcium phosphate powders were prepared by a wet precipitation method using calcium carbonate (CaCO 3 ) and phosphoric acid (H 2 PO 4 ) as starting materials. 27 Briefly, the reagents were mixed at Ca/P ratio of 1.517 corresponding to BCP of 20/80 HA to β-TCP. The precipitate was heated at 80 ℃ for 1 h with stirring, aged for 48 h, washed, filtered and dried at 100 ℃.…”

Section: Methodsmentioning

confidence: 99%

Effect of biphasic calcium phosphate scaffold porosities on odontogenic differentiation of human dental pulp cells

et al. 2016

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Calcium phosphates (CaP) of different porosities have been widely and successfully used as scaffolds with osteoblast cells for bone tissue regeneration. However, the effects of scaffold porosities on cell viability and differentiation of human dental pulp cells for dentin tissue regeneration are not well known. In this study, biphasic calcium phosphate (BCP) scaffolds of 20/80 hydroxyapatite to beta tricalcium phosphate ratio with a mean pore size of 300 μm were prepared into BCP1, BCP2, BCP3, and BCP4 of 25%, 50%, 65%, and 75% of total porosities, respectively. The extracts of these scaffolds were assessed with regard to cell viability, proliferation, and differentiation of human dental pulp cells. The high alkalinity, and more calcium and phosphate ions release that were exhibited by BCP3 and BCP4 decreased the viability and proliferation of human dental pulp cells as compared to BCP1 and BCP2. BCP2 significantly increased both cell viability and cell proliferation. However, the cells cultured with BCP3 extract revealed high alkaline phosphatase (ALP) activity and high expression of odontoblast related genes, collagen type I alpha 1, dentin matrix protein-1, and dentin sialophosphoprotein as compared to that cultured with BCP1, BCP2, and BCP4 extracts. The results highlight the effect of different scaffold porosities on the cell microenvironment and demonstrate that BCP3 scaffold of 65% porosity can support human dental pulp cells differentiation for dentin tissue regeneration.

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A simple pathway in preparation of controlled porosity of biphasic calcium phosphate scaffold for dentin regeneration

Cited by 35 publications

References 33 publications

3D Printing Bioceramic Porous Scaffolds with Good Mechanical Property and Cell Affinity

3D Printing Bioceramic Porous Scaffolds with Good Mechanical Property and Cell Affinity

In VitroandIn VivoStudies of BMP-2-Loaded PCL–Gelatin–BCP Electrospun Scaffolds

Effect of biphasic calcium phosphate scaffold porosities on odontogenic differentiation of human dental pulp cells

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