A simple, optimized method for the determination of sulphide in whole blood by GC–MS as a marker of bowel fermentation processes

Hyspler, R.; Tichá, Alena; Indrová, Monika; Zadák, Z; Hyšplerová, Lidmila; Gasparič, J.; Churáček, J.

doi:10.1016/s1570-0232(01)00632-8

Cited by 71 publications

(35 citation statements)

References 8 publications

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“…The treated serum as described in the experimental section was added into the aqueous dispersion of AgNPs. The average sulfide concentration in three replicate samples was about 16.3 μM, which was consistent with other reported results about sulfide concentrations in serum [39,40]. For the spiked-recovery assays, the results revealed that the recoveries of the added S 2− with the known concentration 5, 10, 20, 30, and 50 μM were 97%, 99%, 103%, 98%, and 99%, respectively, which was acceptable, indicating that it can be used to monitor sulfide in real biological samples including bioassays, nanotechnology, and clinical diagnostics.…”

Section: Resultssupporting

confidence: 92%

A Colorimetric Sensor for the Highly Selective Detection of Sulfide and 1,4-Dithiothreitol Based on the In Situ Formation of Silver Nanoparticles Using Dopamine

Zhao

Miao

et al. 2017

Sensors

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Hydrogen sulfide (H2S) has attracted attention in biochemical research because it plays an important role in biosystems and has emerged as the third endogenous gaseous signaling compound along with nitric oxide (NO) and carbon monoxide (CO). Since H2S is a kind of gaseous molecule, conventional approaches for H2S detection are mostly based on the detection of sulfide (S2−) for indirectly reflecting H2S levels. Hence, there is a need for an accurate and reliable assay capable of determining sulfide in physiological systems. We report here a colorimetric, economic, and green method for sulfide anion detection using in situ formation of silver nanoparticles (AgNPs) using dopamine as a reducing and protecting agent. The changes in the AgNPs absorption response depend linearly on the concentration of Na2S in the range from 2 to 15 μM, with a detection limit of 0.03 μM. Meanwhile, the morphological changes in AgNPs in the presence of S2− and thiol compounds were characterized by transmission electron microscopy (TEM). The as-synthetized AgNPs demonstrate high selectivity, free from interference, especially by other thiol compounds such as cysteine and glutathione. Furthermore, the colorimetric sensor developed was applied to the analysis of sulfide in fetal bovine serum and spiked serum samples with good recovery.

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Section: Resultssupporting

confidence: 92%

A Colorimetric Sensor for the Highly Selective Detection of Sulfide and 1,4-Dithiothreitol Based on the In Situ Formation of Silver Nanoparticles Using Dopamine

Zhao

Miao

et al. 2017

Sensors

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“…A number of methods have been reported for the detection of H 2 S [147,148]. These methods include gas chromatography (GC) [149–152], HPLC [153], and electrochemical methods, which mostly rely on sulfide ion selective electrodes [154,155] or polarographic methods [156]. Despite the availability of numerous detection methods, literature reported hydrogen sulfide concentrations vary significantly among publications, ranging from high micromolar [48,157,158] to low nanomolar [159].…”

Section: Probes For H2smentioning

confidence: 99%

Thiol Reactive Probes and Chemosensors

Peng

Chen

Cheng

et al. 2012

Sensors

257

138

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Thiols are important molecules in the environment and in biological processes. Cysteine (Cys), homocysteine (Hcy), glutathione (GSH) and hydrogen sulfide (H2S) play critical roles in a variety of physiological and pathological processes. The selective detection of thiols using reaction-based probes and sensors is very important in basic research and in disease diagnosis. This review focuses on the design of fluorescent and colorimetric probes and sensors for thiol detection. Thiol detection methods include probes and labeling agents based on nucleophilic addition and substitution, Michael addition, disulfide bond or Se-N bond cleavage, metal-sulfur interactions and more. Probes for H2S are based on nucleophilic cyclization, reduction and metal sulfide formation. Thiol probe and chemosensor design strategies and mechanism of action are discussed in this review.

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“…Most papers suggest that H 2 S is present in the blood at 10-100 lM Savage and Gould 1990;Hyspler et al 2002;Chen et al 2005;Jiang et al 2005;Li et al 2005) while several papers reported undetectable or very low concentrations (\100 nM) Whitfield et al 2008). Compared to most other biologically relevant molecules, H 2 S is both volatile and reactive.…”

Section: Introductionmentioning

confidence: 99%