A Colorimetric Sensor for the Highly Selective Detection of Sulfide and 1,4-Dithiothreitol Based on the In Situ Formation of Silver Nanoparticles Using Dopamine

Abstract

Hydrogen sulfide (H2S) has attracted attention in biochemical research because it plays an important role in biosystems and has emerged as the third endogenous gaseous signaling compound along with nitric oxide (NO) and carbon monoxide (CO). Since H2S is a kind of gaseous molecule, conventional approaches for H2S detection are mostly based on the detection of sulfide (S2−) for indirectly reflecting H2S levels. Hence, there is a need for an accurate and reliable assay capable of determining sulfide in physiological systems. We report here a colorimetric, economic, and green method for sulfide anion detection using in situ formation of silver nanoparticles (AgNPs) using dopamine as a reducing and protecting agent. The changes in the AgNPs absorption response depend linearly on the concentration of Na2S in the range from 2 to 15 μM, with a detection limit of 0.03 μM. Meanwhile, the morphological changes in AgNPs in the presence of S2− and thiol compounds were characterized by transmission electron microscopy (TEM). The as-synthetized AgNPs demonstrate high selectivity, free from interference, especially by other thiol compounds such as cysteine and glutathione. Furthermore, the colorimetric sensor developed was applied to the analysis of sulfide in fetal bovine serum and spiked serum samples with good recovery.

Au/AgI Dimeric Nanoparticles for Highly Selective and Sensitive Colorimetric Detection of Hydrogen Sulfide

“…It is well known that Ag or Cu can be etched by sulfide in the presence of oxygen to form Ag 2 S and CuS. Based on this principle, a series of LSPR nanoprobes, such as Ag NPs, Cu NPs, Ag/Au core–shell nanoprisms, Au@Ag core–shell NPs, and nanorods, were developed for the colorimetric detection of sulfide. However, these Cu‐ and Ag‐based nanoprobes are susceptible to oxidation in the presence of coordination agents, limiting the selectivity of these probes and their use in real samples with complicated matrices.…”

Twinning as a Guiding Factor in Morphological Anisotropy of Silver Nanoparticles Stabilized Over L–DOPA: A Colorimetric Probe for Sulfide in Aqueous Medium

“…While others highlighted the seed mediated as well as seedless synthesis of gold nanorods using dopamine as binding agent along with other additives . Nevertheless few reports cited in the literature do not endorse the formation of varieties of shapes of AgNPs while using dopamine in alkaline aqueous medium, but in the present work a variety of shapes of AgNPs viz., spherical, quasi spherical, truncated triangle, hexagon, rods etc. with majority being quasi spherical under alkaline conditions utilizing L–DOPA have been obtained by us.…”

Colorimetric Sensing of Pb2+ Ion by Using Ag Nanoparticles in the Presence of Dithizone

“…The decrease in the absorbance peak can be attributed to the reaction of AgNPs with the thiourea from Pb(II)-dithizone complex ion. Thiol compounds such as L-cysteine, L-dithiothreitol, 11-mercaptoundecanoic acid, glutathione (GSH), oxidized glutathione (GSSG), and N-acetyl-L-cysteine have a strong effect on the localized surface plasmon resonance of AgNPs, and all decrease the LSPR absorbance of the AgNPs [29]. The observed shift in the wavelength of the LSPR peak absorbance of AgNPs indicates that the sulfur atom of the dithizone interacts with metallic Ag to form an Ag-S covalent bond.…”