A simple optical fiber device for quantitative fluorescence microscopy of single living cells

Graft, M. van; Oosterhuis, Bernard; Werf, Kees O. van der; Grooth, B.G. de; Greve, Jan

doi:10.1016/0022-1759(93)90152-w

Cited by 3 publications

(3 citation statements)

References 15 publications

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“…Xanthene dyes, particularly fluoresceins, ,,,,,, , ,,,, , ,,− (Figure ) are the most commonly used pH probes. Wide commercial availability of various derivatives and conjugates offers easy access to various applications especially in biological and medical research.…”

Section: Overview Of Commonly Used Luminescent Ph Probesmentioning

confidence: 99%

Optical Sensing and Imaging of pH Values: Spectroscopies, Materials, and Applications

2020

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This is the first comprehensive review on methods and materials for use in optical sensing of pH values and on applications of such sensors. The Review starts with an introduction that contains subsections on the definition of the pH value, a brief look back on optical methods for sensing of pH, on the effects of ionic strength on pH values and p K a values, on the selectivity, sensitivity, precision, dynamic ranges, and temperature dependence of such sensors. Commonly used optical sensing schemes are covered in a next main chapter, with subsections on methods based on absorptiometry, reflectometry, luminescence, refractive index, surface plasmon resonance, photonic crystals, turbidity, mechanical displacement, interferometry, and solvatochromism. This is followed by sections on absorptiometric and luminescent molecular probes for use pH in sensors. Further large sections cover polymeric hosts and supports, and methods for immobilization of indicator dyes. Further and more specific sections summarize the state of the art in materials with dual functionality (indicator and host), nanomaterials, sensors based on upconversion and 2-photon absorption, multiparameter sensors, imaging, and sensors for extreme pH values. A chapter on the many sensing formats has subsections on planar, fiber optic, evanescent wave, refractive index, surface plasmon resonance and holography based sensor designs, and on distributed sensing. Another section summarizes selected applications in areas, such as medicine, biology, oceanography, bioprocess monitoring, corrosion studies, on the use of pH sensors as transducers in biosensors and chemical sensors, and their integration into flow-injection analyzers, microfluidic devices, and lab-on-a-chip systems. An extra section is devoted to current challenges, with subsections on challenges of general nature and those of specific nature. A concluding section gives an outlook on potential future trends and perspectives.

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Section: Overview Of Commonly Used Luminescent Ph Probesmentioning

confidence: 99%

Optical Sensing and Imaging of pH Values: Spectroscopies, Materials, and Applications

2020

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“…They have both dual excitation and dual emission properties. Their dual emission properties, in particular, may make them the preferred probes for use in fiber-optic pH sensors [18].…”

Section: Ph-sensitive Dyementioning

confidence: 99%

Observation of mitochondrial activity based on temporal and spatial pH variations measured by near-field fluorescent ratiometry

Shionhara

Iwami

et al. 2011

Sci. China Phys. Mech. Astron.

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A novel method combining dual wavelength fluorescent ratiometry with scanning near-field optical microscopy (SNOM) is proposed and developed to measure the concentration and distribution of protons in the vicinity of biological samples. This method involves immersing mitochondria in a pH-sensitive fluorescent dye solution instead of injecting the dye into the surface of the mitochondrial membrane. It uses a dual emission pH-sensitive dye and SNOM with a thermally pulled and metal-coated optical fiber probe to improve the spatial resolution. The time dependence of the fluorescence intensity ratio (FIR) under acid addition and the response of mitochondria to nutritional supplementation were studied by using this method. Activation of mitochondria and a distance-dependent delay in the FIR response were observed. The results confirmed that mitochondrial activity could be observed by using this method. local pH, pH measurement, SNOM, proton concentration, mitochondrial membrane, ATP synthesis

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“…Many fluorescence methods have been successfully employed in cell biology to, for example, measure calcium levels, 1 pH, 2 etc. Fluorescence resonance energy transfer 3 ͑FRET͒ is potentially one of the most powerful, as its measurement is highly sensitive to the distance between donor and acceptor pairs in the range from 0.5 to about 10 nm.…”

Section: Introductionmentioning

confidence: 99%

A high sensitivity time-resolved microfluorimeter for real-time cell biology

Martin-Fernandez

Tobin

Clarke

et al. 1996

Review of Scientific Instruments

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Articles you may be interested inHighly sensitive real-time detection of DNA hybridization by using nanoporous waveguide fluorescence spectroscopy Appl. Phys. Lett. 105, 031103 (2014); 10.1063/1.4890984Time-resolved x-ray photoelectron spectroscopy techniques for real-time studies of interfacial charge transfer dynamics AIP Conf.We describe an instrument based on the novel combination of synchrotron radiation, a high sensitivity time-resolved microfluorimeter, and a multiframe single photon counting data acquisition system. This instrument has been designed specifically to measure kinetic events in live cells using fluorescence resonance energy transfer, and is capable of rapidly collecting multiple consecutive decay profiles from a small number of fluorophores. The low irradiance on the samples ͑Ͻ10 mW/cm 2 ͒ greatly reduces probe photobleaching and specimen photodamage during prolonged exposures. The Daresbury Synchrotron Radiation Source provides fully wavelength tunable light pulses that have a full width half-maximum of 160 ps at a repetition rate of 3.125 MHz, with the high temporal stability required for continuous measurements over periods of hours. A very low limit of detection ͑Ͻ10 4 molecules/mW/cm 2 ͒ is accomplished by combining a high-gain single photon counting detection system with a low fluorescence background optical layout. The latter is achieved by the inclusion of collimating optics, a reflecting objective, and a specially designed beam stop situated in the epi-fluorescence light-path. A typical irradiance of 8 mW/cm 2 on a sample of ϳ10 5 fluorescein molecules gives, in under 20 s, a fluorescence decay profile with a peak height of 10 4 counts, over 400 channels, and a signal to background ratio better than 40. The data acquisition system has been developed to have a real-time time-resolved fluorescence collection capability ͑denoted as TR 2 ͒ so that fluorescence lifetime data can be continually collected throughout a changing process. To illustrate the potential of this instrument, we present the results of a TR 2 experiment in which lifetime measurements of fluorescence resonance energy transfer are used to monitor the degree of clustering of epidermal growth factor receptors during endocytosis, over a period of about 1 h, with a 5 s resolution.

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A simple optical fiber device for quantitative fluorescence microscopy of single living cells

Cited by 3 publications

References 15 publications

Optical Sensing and Imaging of pH Values: Spectroscopies, Materials, and Applications

Optical Sensing and Imaging of pH Values: Spectroscopies, Materials, and Applications

Observation of mitochondrial activity based on temporal and spatial pH variations measured by near-field fluorescent ratiometry

A high sensitivity time-resolved microfluorimeter for real-time cell biology

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