A simple nutrient-tolerance (NT) test for the characterization of the different types of oligocarbotolerant and oligocarbophile water bacteria from non-carbonated mineral water

Schmidt‐Lorenz, W.; Bischofberger, Th.; Cha, So-Young

doi:10.1016/0168-1605(90)90063-b

Cited by 9 publications

(5 citation statements)

References 18 publications

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“…The presence in the water of oligocarbotolerant, mesotrophic bacteria with relatively slow growth rates and medium nutrient requirements (Table 5) explains the slow development and rapid fall in the microbial counts, in comparison with the findings in other springs (Gonzalez et al 1987a;Schmidt-Lorenz et al 1990). The counts in the C samples did not exceed lo3 after storage for 7 weeks, which is a much smaller number than counts cited by other workers.…”

Section: Bacterlal Counts During Storage According To Bottling Proceduresmentioning

confidence: 60%

“…Suitable plate count media and appropriate techniques for enumerating bacteria have been proposed by other workers (Taylor et al 1981;Reasoner & Geldreich 1985;Oger et al 1987;Rivilla & Gonzalez 1988;Schmidt-Lorenz et al 1990). Diluted plate count agar (PCA) and a surface plating technique seem to be better than full strength PCA and pour plates for enumerating the autochthonous microflora.…”

Section: Introductionmentioning

confidence: 99%

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Study of the bacterial flora of a non‐carbonated natural mineral water

Mavridou

1992

Journal of Applied Bacteriology

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Natural mineral water from a UK spring was monitored at various stages after it was pumped from the ground, through to bottling and during shelf life before consumption. Samples were collected in commercial PVC bottles, in PVC bottles previously sterilized and hand-filled and in glass bottles. The bacterial flora was counted on plate count agar (PCA) and on PCA diluted 10 times (PCA/10). The predominant bacteria were identified to genus level. Growth rates and nutrient types of isolates were determined by the nutrient-tolerance test (NT). The plate counts at the pre-bottling stage were low. During storage larger numbers of bacteria grew in glass than PVC bottles; the largest number grew in PVC bottles filled by hand. Most of the pigmented bacteria isolated were oligocarbotolerant.

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Section: Bacterlal Counts During Storage According To Bottling Proceduresmentioning

confidence: 60%

Section: Introductionmentioning

confidence: 99%

Study of the bacterial flora of a non‐carbonated natural mineral water

Mavridou

1992

Journal of Applied Bacteriology

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“…Ž . Previous studies Schmidt-Lorenz et al, 1990 demonstrated that various isolates from bottled, non-carbonated, natural mineral water had an average optimum temperature of 268C. It is, however, recommended to use incubation at 308C since all the indicator pathogens investigated throughout this Ž EC-funded study Escherichia coli, Pseudomonas aeruginosa, Salmonella typhimurium, Staphylococ-.…”

Section: Resultsmentioning

confidence: 99%

Improved methods for the enumeration of heterotrophic bacteria in bottled mineral waters

Ramalho

Cunha

Teixeira

et al. 2001

Journal of Microbiological Methods

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At this time the European Union regulations require that the heterotrophic plate counts (HPC) of mineral waters be assessed at two recovery temperatures: 22 degrees C for 72 h and 37 degrees C for 24 h. This procedure is time consuming and expensive. Development of new rapid methods for microbiological assessment of the microbial flora in the bottled water is an industry-driven need. The objectives of this work were to develop a method for the HPC that utilises only one recovery temperature and one incubation period and evaluate the use of, the LIVE/DEAD(R) BacLight Bacterial Viability Kit, 5-cyano-2,3-ditotyl tetrazolium chloride (CTC) and impedance methods to enumerate viable bacteria in bottled mineral water. Results showed that incubation at 30 degrees C could be used instead of incubation at 22 degrees C and 37 degrees C. Good correlation exists between counts at 30 degrees C and counts at 22 degrees C (r>0.90) and all the pathogens important in mineral water analyses grow similarly at 30 degrees C and 37 degrees C during 24 h. It was demonstrated that impedance methods might be useful to the mineral water industry as a rapid indicator of microbiological quality of the water. Results obtained with BacLight and CTC were similar to those obtained with plate counts.

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“…Além disso, P. aeruginosa, pode alterar cor, turbidez e sabor de águas [25]. Algumas espécies de Pseudomonas isoladas de águas minerais, são capazes de se multiplicarem e atingirem populações de 10 4 -10 5 UFC/mL quando inoculadas em água mineral esterilizada [23].…”

Section: Warburton Austin Harrisson and Sandersunclassified