A simple method of cartilage regeneration using a new polymerizing system: ultrastructural characteristics of the repair tissue

Reissis, N.; Downes, S.; Kayser, M. V.; Bentley, G.

doi:10.1007/bf00213137

Cited by 19 publications

(10 citation statements)

References 14 publications

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“…9 An alternative approach is to use another polymer system, such as the heterocyclic methacrylate described by Patel et al, 10 which consists of poly(ethyl methacrylate) polymer and tetrahydrofurfuryl methacrylate monomer (PEMA/THFMA) that together form a semi-interpenetrating network with low polymerization shrinkage. 10 In the current study we have extended previous work with this material in dental pulp, 11 cartilage, 12 and chondrocytes 13 to examine the attachment of human bone-derived cells (HBDC).…”

Section: Introductionmentioning

confidence: 97%

Attachment of cultured human bone cells to novel polymers

McFarland

Mayer

Scotchford

et al. 1999

J. Biomed. Mater. Res.

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The initial attachment of human bone-derived cells (HBDC) to several polymer systems has been studied in vitro. A novel polymer system based on poly(ethyl methacrylate) polymer and tetrahydrofurfuryl methacrylate monomer (PEMA/THFMA) was compared with a variant in which THFMA was replaced by 2-hydroxyethyl methacrylate (PEMA/HEMA). Tissue culture polystyrene (TCPS) and polystyrene (PS) were used as reference materials. The ability of the substrates to adsorb the attachment glycoproteins fibronectin (Fn) and vitronectin (Vn) from serum and the subsequent effect on radiolabeled HBDC attachment were examined. Initial cell attachment from the medium containing 10% (v/v) serum was highest on TCPS; on PEMA/THFMA and PEMA/HEMA substrates it was about 25% of this level, and on PS it was only 10% of that on TCPS. Attachment of HBDC to all substrates was dependent on the presence of Vn, which, unlike Fn, was able to adsorb in the face of competition from other serum components. Both Vn and Fn were able to support cell attachment when precoated onto all substrates. In comparison to TCPS, PEMA/THFMA did not show enhanced adsorption of either Fn or Vn from serum, and this was reflected in the level of cell attachment. Interestingly, the potency of preadsorbed Fn for cell attachment was much higher on this substrate than on any other: 45 ng/cm2 Fn when adsorbed to PEMA/THFMA gave a level of cell attachment 1.6-fold higher than the same density of Fn on PS or TCPS. The maximum Fn surface density achieved on HEMA/PEMA was 16 ng/cm2. Cells on PEMA/THFMA showed typical clustering of the alpha5 beta1 Fn receptor, but this was not evident in cells attached to PEMA/HEMA even when precoated with Fn. This study indicates that the initial attachment of HBDC to all substrates was Vn dependent. It also indicates that on PEMA/THFMA the favorable presentation of subsequently adsorbed Fn may assist matrix assembly.

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Section: Introductionmentioning

confidence: 97%

Attachment of cultured human bone cells to novel polymers

McFarland

Mayer

Scotchford

et al. 1999

J. Biomed. Mater. Res.

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“…The resulting fibrocartilaginous repair tissue is a poor substitute for articular cartilage and is prone to failure, particularly at interfaces between repair and host tissue (9). Many studies have attempted to enhance this reparative response through the addition of growth factors (10,11) or by the insertion of synthetic matrices, either alone (12)(13)(14) or seeded with allogenic chondrocytes or mesenchymal stem cells (15)(16)(17). Other studies have explored the introduction of allografts of articular cartilage into the wound (18) and, more recently, autologous chondrocyte transplantation, which maintains the cells within the lesion by the aid of a periosteal flap (19,20).…”

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confidence: 99%

The reactions of articular cartilage to experimental wounding: Role of apoptosis

Tew

Kwan

Hann

et al. 2000

Arthritis & Rheumatism

229

170

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Objective. To determine the cellular and matrix responses to experimental wounding of articular cartilage.Methods. Immature and mature bovine articular cartilage was used as an in vitro model system to study the cellular responses to cartilage wounding. Explant cultures were wounded centrally with a trephine and maintained for up to 10 days. TUNEL labeling together with ultrastructural analyses were used to assess the nature of the observed cell death. In vitro labeling with 3 H-thymidine was used to detect cell proliferation, and 2 antibodies (COL2-3/4M and BC-13) were used to detect changes in matrix turnover.Results. Cell death was observed as a response to wounding and was considered to be a combination of necrosis and apoptosis. In immature tissue, cell death was more pronounced, particularly in the articular surface region. Within the area of cell death, many cells that did not die subsequently underwent proliferation. The collagenous network showed evidence of denaturation in the area of the wound, but "aggrecanase" activity was not detected.Conclusion. There are 2 contrasting, but related, responses to cartilage wounding-apoptosis and proliferation. In order to improve cartilage repair, future studies need to elucidate the regulatory mechanisms that determine these responses.

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“…It has been suggested [6], that PEMA/THFMA increases proteoglycan synthesis by the chondrocytes, thus increasing resistance to compressive mechanical loading, applied to the repairing defect. It has also been shown [16] that the capability of the articular cartilage to repair is related to s'tress placed upon it and that excessive loading can be harmful.…”

Section: Discussionmentioning

confidence: 99%