A hydrophylic polymer system enhanced articular cartilage regeneration in vivo

Reissis, N.; Kayser, M. V.; Bentley, G.; Downes, S.

doi:10.1007/bf00134315

Cited by 9 publications

(7 citation statements)

References 17 publications

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“…The scaffold's physical and chemical characteristics determine whether the seeded cells will grow and maintain their morphology and phenotype. [51][52][53] These characteristics include the texture of the scaffold surfaces contacting the cells, the presence of pores, the pore structure, size, and distribution, the presence of chemical groups controlling surface hydrophilicity (positively contributes to the expression of the chondrocyte phenotype), surface free energy, and the ability to form ionic bonds with cells. 39,[51][52][53] These are strongly influenced by both the quality of the polymer and the technique used for scaffold preparation.…”

Section: Discussionmentioning

confidence: 99%

In vitro growth and activity of primary chondrocytes on a resorbable polylactide three-dimensional scaffold

Gugala

Gogolewski

2000

J. Biomed. Mater. Res.

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Sheep articular chondrocytes were cultured for 3, 6, and 9 weeks on a three-dimensional porous scaffold from poly(L/DL-lactide) 80/20%. Cell growth and activity was estimated from the amount of proteoglycans attached to the polylactide scaffold and the amounts of DNA and proteins measured in the cell lysate. Cell morphology was assessed from scanning electron microscopy. Histochemical staining of proteoglycans present in the sponge was used to visualize the chondrocyte ingrowth in the scaffold. The amounts of DNA, proteins, and proteoglycans increased with time of culturing. Chondrocytes on the polylactide scaffold maintained their round shape. The cell ingrowth into the sponge progressed with time of culturing and proceeded from the upper surface of the sponge toward its lower surface. At 9 weeks, the chondrocytes filled the whole scaffold and reached the opposite side of the sponge. The proteoglycans network was, however, more dense at the upper half of the scaffold.

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Section: Discussionmentioning

confidence: 99%

In vitro growth and activity of primary chondrocytes on a resorbable polylactide three-dimensional scaffold

Gugala

Gogolewski

2000

J. Biomed. Mater. Res.

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“…We have previously reported on a new heterocyclic methacrylate, based on poly-ethyl-methacrylate (PEMA) polymer powder and tetra-hydro-furfurylmethacrylate (THFMA) monomer liquid, that appeared to enhance the biological resurfacing in large, full-thickness articular cartilage defects in a rabbit model [10]. The self-repair potential of the rabbits was also validated in the same study; the control empty holes repaired with significantly inferior tissue, both in structure and composition.…”

Section: Introductionmentioning

confidence: 89%

Characterization of the repair tissue in articular cartilage defects using silver-enhanced colloidal gold immunostaining

Reissis

Downes

Kayser

et al. 1994

J Mater Sci: Mater Med

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The newly developed silver-enhanced colloidal gold staining method was used in a rabbit model to characterize the repair tissue in large articular cartilage defects filled with a heterocyclic methacrylate polymer. By 6 weeks the resurfacing tissue consisted of highly organized hyaline-like articular cartilage, fully integrated with the adjacent normal cartilage. Immuno-histochemistry detected collagen type II, keratan sulphate, chondroitin 4-sulphate and chondroitin 6-sulphate in the matrix of the neocartilage. The level to which the polymer plug was recessed appeared to be critical to the overall quality of the repair tissue. Optimum results were obtained when the top surface of the biomaterial was at the level of the subchondral bone, below the level of the surrounding articular cartilage. Other technical aspects of implantation, that also affect the repair, are also discussed.

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“…In both the cases, polymers are transformed from the liquid state into the solid state (phase‐inverse process) using various techniques. The techniques involve particulate‐leaching, freeze‐drying, solid free‐form fabrication, nonwoven web‐spinning, gas‐foaming, and eutectic crystallization 1–33. Scaffolds produced using nonwoven web‐spinning consisting of microfibers are soft and floppy.…”

Section: Introductionmentioning

confidence: 99%

Biodegradable porous polyurethane scaffolds for tissue repair and regeneration

Górna

Gogolewski

2006

J Biomedical Materials Res

156

104

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Critical-size bone defects usually require the insertion of autogenous bone graft to heal. Harvesting of bone is traumatic and results in high morbidity at the donor site. A potential alternative to bone graft may be a bone substitute with adequate biocompatibility and biological properties produced from ceramics or bioresorbable/biodegradable polymers. In the present study, new elastomeric biodegradable polyurethanes with an enhanced affinity toward cells and tissues were synthesized using aliphatic diisocyanate, poly(epsilon-caprolactone) diol, and biologically active 1,4:3,6-dianhydro-D-sorbitol (isosorbide diol) as chain extender. The polymers were processed into 3D porous scaffolds by applying a combined salt leaching-phase inverse process. The critical parameters controlling pore size and geometry were the solvents and nonsolvents used for scaffold preparation and the sizes of the solid porogen crystals. Scaffolds prepared from the polymer solution in solvents such as dimethylsulfoxide or methyl-2-pyrrolidone did not have a homogenous pore structure. Many pores were interconnected, but numerous pores were closed. Irrespective of the high pore-to-volume ratio (75%), the scaffolds showed poor water permeability. The best solvent for the preparation of scaffolds from the polyurethane used in the study was dimethylformamide (DMF). The type of nonsolvent admixed to the polymer solution in DMF strongly affected the scaffolds' pore structure. The elastomeric polyurethane scaffold prepared from the optimal solvent-nonsolvent mixture had regular interconnected pores, high water permeability, and a pore-to-volume ratio of 90%. The osteoconductive properties of the 3D porous polyurethane scaffolds can be additionally promoted by loading them with calcium phosphate salts such as hydroxyapatite or tricalcium phosphate, thus making them promising candidates for bone graft substitutes.

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A hydrophylic polymer system enhanced articular cartilage regeneration in vivo

Cited by 9 publications

References 17 publications

In vitro growth and activity of primary chondrocytes on a resorbable polylactide three-dimensional scaffold

In vitro growth and activity of primary chondrocytes on a resorbable polylactide three-dimensional scaffold

Characterization of the repair tissue in articular cartilage defects using silver-enhanced colloidal gold immunostaining

Biodegradable porous polyurethane scaffolds for tissue repair and regeneration

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