A simple method for spore germination studies

Odell, D.E.; Smith, Jason

doi:10.1016/s0007-1528(82)80012-6

Cited by 5 publications

(2 citation statements)

References 10 publications

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“…All assays had a final spore concentration of 5 × 10 6 conidia/ml. All the above incubation treatments were performed by placing 25 μl of the freshly mixed spore-PDB/H 2 O suspension onto each slide and placing the slides in a Petri dish with a moistened filter paper (Odell & Smith, 1982). Conidial germination was examined under a light microscope (400×) 3, 6, 9, 12, 24, and 36 hr after incubation.…”

Section: Effect Of Different Environmental Conditions On Conidial Germinationmentioning

confidence: 99%

Asexual reproduction and vegetative growth of Bionectria ochroleuca in response to temperature and photoperiod

Zheng

Xie

et al. 2021

Ecology and Evolution

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Phenotypic plasticity is the ability of a given genotype to produce different phenotypes in various environments (Holloway, 2002) and is a fundamental characteristic of all organisms, enabling adaptation to various environments (Slepecky & Starmer, 2009). The molecular basis of phenotypic plasticity is differential gene expression triggered by environmental changes (Alvarez et al., 2015;Gibson, 2008). Such regulation includes early signal responses, signal transduction, and gene expression regulation (Lengeler et al., 2000). Gene expression is regulated at various stages, in-

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Section: Effect Of Different Environmental Conditions On Conidial Germinationmentioning

confidence: 99%

Asexual reproduction and vegetative growth of Bionectria ochroleuca in response to temperature and photoperiod

Zheng

Xie

et al. 2021

Ecology and Evolution

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“…An aliquot of 4 μl of 250 ng/μl Cy3‐labelled GFP dsRNA was mixed with 4 μl of fresh rust spores (10 4 –10 6 spores/ml) and plated on 1% water agar drops on microscope slides or directly on the microscope slides, according to the method described by Odell and Smith ( 1982 ). Slides were placed on moist filter paper in a Petri dish sealed with Parafilm.…”

Section: Methodsmentioning

confidence: 99%

Exogenous double‐stranded RNA inhibits the infection physiology of rust fungi to reduce symptoms in planta

Degnan

McTaggart

Shuey³

et al. 2022

Molecular Plant Pathology

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Rust fungi (Pucciniales) are a diverse group of plant pathogens in natural and agricultural systems. They pose ongoing threats to the diversity of native flora and cause annual crop yield losses. Agricultural rusts are predominantly managed with fungicides and breeding for resistance, but new control strategies are needed on non-agricultural plants and in fragile ecosystems. RNA interference (RNAi) induced by exogenous double-stranded RNA (dsRNA) has promise as a sustainable approach for managing plant-pathogenic fungi, including rust fungi. We investigated the mechanisms and impact of exogenous dsRNA on rust fungi through in vitro and whole-plant assays using two species as models, Austropuccinia psidii (the cause of myrtle rust) and Coleosporium plumeriae (the cause of frangipani rust). In vitro, dsRNA either associates externally or is internalized by urediniospores during the early stages of germination. The impact of dsRNA on rust infection architecture was examined on artificial leaf surfaces. dsRNA targeting predicted essential genes significantly reduced germination and inhibited development of infection structures, namely appressoria and penetration pegs. Exogenous dsRNA sprayed onto 1-year-old trees significantly reduced myrtle rust symptoms. Furthermore, we used comparative genomics to assess the wide-scale amenability of dsRNA to control rust fungi. We sequenced genomes of six species of rust fungi, including three new families (Araucariomyceaceae, Phragmidiaceae, and Skierkaceae) and identified key genes of the RNAi pathway across 15 species in eight families of Pucciniales. Together, these findings

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Compatability of Metarhizium anisopliae var. anisopliae with chemical pesticides

1987

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The effects of various insecticides on the mycelial growth, sporulation and conidial germination of Metarhizium anisopliae var. anisopliae isolate E9 were studied in the laboratory. Chlorpyrifos was the most toxic organophosphate to mycelial growth and sporulation at all concentrations. Temephos, malathion and leptophos were highly toxic to sporulation while malathion was the most inhibitory to germination. The carbamates, carbofuran, methomyl and oxamyl were moderately toxic to mycelial growth and sporulation while oxamyl had an adverse effect on germination. The pyrethroids (pyrethrin, permethrin and resmethrin) and the insect growth regulators (diflubenzuron and methoprene) were not inhibitory to the various developmental stages of isolate E9. The chlorinated hydrocarbons (chlordane, lindane and toxaphene) were more deleterious than all other insecticide groups tested. Among the fungicides, benomyl and maneb produced the greatest inhibition.

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A simple method for spore germination studies

Cited by 5 publications

References 10 publications

Asexual reproduction and vegetative growth of Bionectria ochroleuca in response to temperature and photoperiod

Asexual reproduction and vegetative growth of Bionectria ochroleuca in response to temperature and photoperiod

Exogenous double‐stranded RNA inhibits the infection physiology of rust fungi to reduce symptoms in planta

Compatability of Metarhizium anisopliae var. anisopliae with chemical pesticides

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