A simple method for quantitative determination of ATP in soil

Eiland, F.

doi:10.1016/0038-0717(83)90030-5

Cited by 72 publications

(22 citation statements)

References 18 publications

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“…NRB consisted of a 0.05% solution of alkyldimethylbenzylammonium chloride in Tris-Mg 2ϩ buffer (20 mM Tris, 2 mM EDTA, and 10 mM Mg 2ϩ [added as Mg acetate]; adjusted to pH 7.75 with acetic acid) (9,29,30). NRB was freshly prepared before each use.…”

Section: Methodsmentioning

confidence: 99%

Variation in Bacterial ATP Level and Proton Motive Force Due to Adhesion to a Solid Surface

Hong

Brown

2009

Appl Environ Microbiol

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Bacterial adhesion to natural and man-made surfaces can be beneficial or detrimental, depending on the system at hand. Of vital importance is how the process of adhesion affects the bacterial metabolic activity. If activity is enhanced, this may help the cells colonize the surface, whereas if activity is reduced, it may inhibit colonization. Here, we report a study demonstrating that adhesion of both Escherichia coli and Bacillus brevis onto a glass surface resulted in enhanced metabolic activity, assessed through ATP measurements. Specifically, ATP levels were found to increase two to five times upon adhesion compared to ATP levels in corresponding planktonic cells. To explain this effect on ATP levels, we propose the hypothesis that bacteria can take advantage of a link between cellular bioenergetics (proton motive force and ATP formation) and the physiochemical charge regulation effect, which occurs as a surface containing ionizable functional groups (e.g., the bacterial cell surface) approaches another surface. As the bacterium approaches the surface, the charge regulation effect causes the charge and pH at the cell surface to vary as a function of separation distance. With negatively charged surfaces, this results in a decrease in pH at the cell surface, which enhances the proton motive force and ATP concentration. Calculations demonstrated that a change in pH across the cell membrane of only 0.2 to 0.5 units is sufficient to achieve the observed ATP increases. Similarly, the hypothesis indicates that positively charged surfaces will decrease metabolic activity, and results from studies of positively charged surfaces support this finding.Bacterial adhesion and biofilm formation are important to a wide array of fields, such as environmental, chemical, and biomedical engineering; food processing; materials science; marine science; and ecology and environmental sciences. A key consideration in the development of a biofilm is the initial interaction between the bacterium and the substrata and the way in which these interactions affect the metabolic activity of the cells. If the metabolic activity increases, this would help the cells colonize the surface, whereas if the metabolic activity decreases, then the cells may become inactive or die.There have been a number of studies that have examined the effects of attachment on bacterial metabolic activity. The first reported observation of this effect was made in 1943, where it was found that bacterial activity increased in the presence of glass surfaces, particularly with low nutrient concentrations (54). Since this first study, researchers have examined how various materials, including glass and polymer surfaces (10, 11, 24-26, 40, 44, 47), silicone surfaces (52), ceramic surfaces (32), dialysis membranes (18), activated carbon (7), clays (43), sands (31, 48), estuary particles (19), and ion exchange resins (46), affect the metabolic activity of attached bacteria.Different mechanisms have been examined in an attempt to explain how surfaces affect bacterial metabo...

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Section: Methodsmentioning

confidence: 99%

Variation in Bacterial ATP Level and Proton Motive Force Due to Adhesion to a Solid Surface

Hong

Brown

2009

Appl Environ Microbiol

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“…To convert substrate-induced respiration rate to biomass C, a conversion factor asuming that 100 ^ig CO 2 h~' was equivalent to 2 mg of biomass C was used (Anderson & Domsch 1978). The second method was soil ATP content, analysed after extraction of 0.5 g wet soil with 10 ml of 500 mM H 2 SO 4 -250 mM Na 2 HPO 4 according to Arnebrant & BÅÅth (1991) based on the method described by Eiland (1983). For calculation of microbial biomass C from ATP content, a conversion factor of 2. l^g ATP mg" 1 biomass C was used (Arnebrant & BÅÅth 1991).…”

Section: Methodsmentioning

confidence: 99%

Soil microbial activity in eleven Swedish coniferous forests in relation to site fertility and nitrogen fertilization

Arnebrant

Bååth

Söderström

et al. 1996

Scandinavian Journal of Forest Research

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“…To estimate total microbial biomass, studies used chloroform fumigation extraction or incubation for microbial C (Brooks et al 1985), total amounts of phospholipid fatty acids (PLFAs) in soil (Frostegard and Bååth 1996), substrate-induced respiration (Anderson and Domsch 1978), or total abundance of ATP in soil (Eiland 1983). Studies characterized fungal abundance using microscopy, fungal-specific PLFAs, total amounts of ergosterol in soil (Djajakirana et al 1996), and quantitative PCR with universal fungal primers (Borneman and Hartin 2000).…”

Section: Indices Of Microbial Abundancementioning

confidence: 99%

The effect of fire on microbial biomass: a meta-analysis of field studies

2011

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Soil microbes regulate the transfer of carbon (C) from ecosystems to the atmosphere and in doing so influence feedbacks between terrestrial ecosystems and global climate change. Fire is one element of global change that may influence soil microbial communities and, in turn, their contribution to the C dynamics of ecosystems. In order to improve our understanding of how fire influences belowground communities, we conducted a meta-analysis of 42 published microbial responses to fire. We hypothesized that microbial biomass as a whole, and fungal biomass specifically, would be altered following fires. Across all studies, fire reduced microbial abundance by an average of 33.2% and fungal abundance by an average of 47.6%. However, microbial responses to fire differed significantly among biomes and fire types. For example, microbial biomass declined following fires in boreal and temperate forests but not following grasslands fires. In addition, wildfires lead to a greater reduction in microbial biomass than prescribed burns. These differences are likely attributable to differences in fire severity among biomes and fire types. Changes in microbial abundance were significantly correlated with changes in soil CO 2 emissions. Altogether, these results suggest that fires may significantly decrease microbial abundance, with corresponding consequences for soil CO 2 emissions.

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A simple method for quantitative determination of ATP in soil

Cited by 72 publications

References 18 publications

Variation in Bacterial ATP Level and Proton Motive Force Due to Adhesion to a Solid Surface

Variation in Bacterial ATP Level and Proton Motive Force Due to Adhesion to a Solid Surface

Soil microbial activity in eleven Swedish coniferous forests in relation to site fertility and nitrogen fertilization

The effect of fire on microbial biomass: a meta-analysis of field studies

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