A simple method for detecting mycoplasma infection of cell cultures

Harnett, Gerald B.; Phillips, P. A.; Mackay‐Scollay, E. M.

doi:10.1136/jcp.27.1.70

Cited by 7 publications

(1 citation statement)

References 18 publications

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“…The organisms did not stain with Brown and Brenn Gram stain but they were stained blue in Wolbach-Giemsa-stained sections, features which are consistent with Mycoplasma. 10 Histologic examination of the lung revealed diffuse alveolar edema, and a fibrous tag was present on the epicardial surface. Histologic lesions were not present in any other tissue.…”

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Meningitis caused by Mycoplasma mycoides subspecies capri in a goat

et al. 2011

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Mycoplasmas are the cause of serious diseases in human beings and animals (e.g., walking pneumonia in human beings; contagious bovine pleuropneumonia, arthritis, and mastitis in cattle; enzootic pneumonia in pigs; and infectious anemia in cats). In goats, mycoplasmosis can manifest in many different ways, ranging from pneumonia and joint pain, to agalactia, pleuropneumonia, or death. Meningitis or central nervous system (CNS) involvement is an uncommon manifestation of mycoplasmosis, reported occasionally in human beings and other animals. Most notably, Mycoplasma pneumoniae infection has been associated with encephalitis, aseptic meningitis, acute transverse myelitis, stroke, and polyradiculopathy. 21 In goats, CNS involvement appears to be even less common, with only 4 naturally occurring cases reported in the literature, all occurring in goat kids. These cases occurred in goat herds in Hungary, Canada, and California, where goats exhibited the more usual systemic lesions such as polyarthritis, pneumonia, and mastitis, in addition to meningitis. 2,14,18 The present paper describes a naturally occurring case of meningitis in an adult Toggenburg goat from which only Mycoplasma mycoides subspecies capri was recovered, and in which there was no evidence of pneumonia or other systemic lesions.A 2-year-old, 3-month-pregnant, female Toggenburg goat was submitted to the Connecticut Veterinary Medical Diagnostic Laboratory (Storrs, Connecticut) for postmortem examination. This goat was a member of a herd of 19 adults and 11 kids used for milking and show. At different time points throughout the past year, the herd had 4 adult goats with neurologic disease including facial paralysis and circling. One of the 4 neurologic goats had a history of a lump at the base of the ear. Two goats recovered after treatment with antibiotics and palliative care; another was euthanized but a necropsy was not performed. The goat in the current report had a 5-day history of pyrexia and intermittent neurologic signs, including nystagmus, seizures, and circling, followed by death.At necropsy, the goat was in good body condition. The subarachnoid space contained diffusely distributed liquid and cream-colored pus; the lungs were diffusely, mildly congested; and a fibrous tag was present on the surface of the heart. No other gross lesions were present. In particular, no lesions were present in the ear canals or tympanic bullae. Tissue samples of brain, spinal cord, trachea, lung, liver, kidney, urinary bladder, heart, rumen, reticulum, omasum, abomasum, small intestine, and large intestine were fixed in 10% neutral buffered formalin, processed routinely, and embedded in paraffin. Sections were cut at 4-µm thickness and stained with hematoxylin and eosin, Brown and Brenn Gram stain, and Wolbach-Giemsa stains. Abstract. A 2-year-old, female goat from Connecticut was submitted for necropsy with a 5-day history of pyrexia and intermittent neurologic signs, including nystagmus, seizures, and circling. Postmortem examination revealed suppurative meni...

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confidence: 99%