Influence of Cell Culture Medium on the Metabolic Behaviour of the Fibroblast

Lie, Sverre O.

doi:10.1007/978-94-011-6627-0_2

The Cultured Cell and Inherited Metabolic Disease 1977

DOI: 10.1007/978-94-011-6627-0_2

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Influence of Cell Culture Medium on the Metabolic Behaviour of the Fibroblast

Sverre O. Lie¹

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1985

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Reversible change in the fibroblast lysosomal enzyme dipeptidyl aminopeptidase-1 (cathepsin c) related to the commercial source of fetal bovine serum in the culture medium

Doughty

Davis

Gruenstein

1985

In Vitro Cell Dev Biol

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The commercial source of fetal bovine serum used to supplement the growth medium of human skin fibroblasts alters the activity of the lysosomal enzyme dipeptidyl aminopeptidase-1 (DAP-1). Cells grown with one serum were found to have a threefold higher level of DAP-1 than those grown with serum from another source (P less than 0.001). The effect on DAP-1 activity was specific inasmuch as no differences were found in the activities of a variety of other lysosomal and nonlysosomal hydrolases: DAP-II, DAP-III, DAP-IV, beta-glucosidase, beta-glucuronidase, and N-acetyl-beta-galactosaminidase. The effect is reversible and is observed over a wide range of cell population doublings. Cell growth kinetics were not significantly different with the different sera.

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Reversible change in the fibroblast lysosomal enzyme dipeptidyl aminopeptidase-1 (cathepsin c) related to the commercial source of fetal bovine serum in the culture medium

Doughty

Davis

Gruenstein

1985

In Vitro Cell Dev Biol

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show abstract

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Influence of Cell Culture Medium on the Metabolic Behaviour of the Fibroblast

Cited by 1 publication

References 53 publications

Reversible change in the fibroblast lysosomal enzyme dipeptidyl aminopeptidase-1 (cathepsin c) related to the commercial source of fetal bovine serum in the culture medium

Reversible change in the fibroblast lysosomal enzyme dipeptidyl aminopeptidase-1 (cathepsin c) related to the commercial source of fetal bovine serum in the culture medium

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