A simple method for deriving functional MSCs and applied for osteogenesis in 3D scaffolds

Zou, Lijin; Luo, Yonglun; Chen, Muwan; Wang, Gang; Ding, Ming; Petersen, Charlotte Christie; Kang, Ran; Dagnæs‐Hansen, Frederik; Zeng, Yanjun; Lv, Nonghua; Ma, Qing; Le, Dang Quang Svend; Besenbacher, Flemming; Bolund, Lars; Jensen, Thomas G.; Kjems, Jørgen; Pu, William T.; Bünger, Cody

doi:10.1038/srep02243

Cited by 112 publications

(147 citation statements)

References 33 publications

Supporting

Mentioning

138

Contrasting

Order By: Relevance

“…The safety of iPS-NCLC-MSCs further confi rmed the reduction of some pluripotency genes as shown in our previous study of iPSNCLCs 12) . Consistent with our results, recent studies related to MSCs derived from iPS cells have demonstrated their safety for clinical application 32,[34][35][36] , providing evidence to promote the use of iPS-NCLCMSCs for stem cell therapy. Interestingly, in iPS-NCLC-MSCs in the present study, Sox2 expression level was unchanged compared to that for undiff erentiated iPS cells.…”

Section: Gfp Osx Mergesupporting

confidence: 80%

“…In addition to Sox2, the relationship between other genes and tumorigenesis has been under debate. Zou et al found that iPS-derived MSCs were positive for Nanog despite the exclusion of other pluripotent markers 34) . Lee et al documented that polysialic acid-neural cell adhesion molecule (PSA-NCAM)-negative NC cells derived from human ESCs were tumorigenic 39) .…”

Section: Gfp Osx Mergementioning

confidence: 99%

See 1 more Smart Citation

Craniofacial Bone Regeneration using iPS Cell-Derived Neural Crest Like Cells

Kikuchi

Masuda

Fujiwara

et al. 2018

J. Hard Tissue Biology.

View full text Add to dashboard Cite

Induced pluripotent stem (iPS) cells represent a powerful source for cell-based tissue regeneration because they are patient-specific cells and can differentiate into specialized cell types. Previously, we have demonstrated the derivation of neural crest like cells from iPS cells (iPS-NCLCs), and these cells have the potential to diff erentiate into dental mesenchymal cells, which subsequently diff erentiate into odontoblasts and dental pulp cells. In this study, we show that iPS-NCLCs can diff erentiate into mesenchymal stem cells (iPS-NCLC-MSCs), which contribute to craniofacial bone regeneration. iPS-NCLCs were cultured in serum-containing media and diff erentiated into functional MSCs, as confi rmed by expression MSC markers and their ability to diff erentiate into osteoblasts, adipocytes, and chondrocytes in vitro. iPS-NCLC-MSCs were negative for markers of undiff erentiated iPS cells and did not develop into teratomas when transplanted to immunodefi cient mice. Further, iPS-NCLC-MSCs grew normally and diff erentiated into osteoblasts on hydroxyapatite scaff olds in vitro. To assess the potential of iPS-NCLC-MSCs to regenerate craniofacial bone in vivo, iPS-NCLC-MSCs were transplanted into critical-size calvarial defects in immunodefi cient mice for 8 weeks. Histological analysis revealed that iPS-NCLC-MSCs diff erentiated into osteoblasts and contributed to bone regeneration without tumor formation. These results indicate that iPS-NCLC-MSCs could be a potential candidate for cell-based craniofacial bone tissue repair and regeneration.

show abstract

Section: Gfp Osx Mergesupporting

confidence: 80%

Section: Gfp Osx Mergementioning

confidence: 99%

Craniofacial Bone Regeneration using iPS Cell-Derived Neural Crest Like Cells

Kikuchi

Masuda

Fujiwara

et al. 2018

J. Hard Tissue Biology.

View full text Add to dashboard Cite

show abstract

“…But, mechanism of why functional MSCs, or mesenchymal progenitor cells named by Yen et al in their study, can efficiently derived from iPSCs by serial culturing and trypsinization needs further investigations 29) . Zou et al showed one possibility, it might be associated with the used serum, but speculated that the serum effect should not be main effector 26) . In this experiment, it was able to be osteoinducted with the cells passaged repeatedly by trypsinization in vitro, but in vivo there was teratoma formation.…”

Section: Discussionmentioning

confidence: 99%

“…Embryoid body-independent mesenchymal differentiation methods have been developed and optimized during the last few years to facilitate the induction of MSCs from human ESCs/iPSCs 27) . Lim et al applied a modified method by culturing single hESCs on gelatin-coated plates in a conditional that are suitable for MSCs growth, which has greatly improved the efficiency of deriving functional MSCs [26][27][28] . Repeated passaging by trypsinization has been showed to enriched MSCs population 28) .…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Differentiation behavior of iPS cells cultured on PLGA with osteoinduction medium

et al. 2017

View full text Add to dashboard Cite

In the present report, we have generated osteoblast-like cells derived from mouse induced-pluripotent stem (iPS) cells on PLGA with osteoinduction medium in vitro and in vivo. The cell culture period was 2 weeks. At 2 weeks, mRNA level of type I collagen was significantly higher than at 1 week. Osteocalcin mRNA level at 2 weeks was tendency to increase compared with at 1 week. And the cells cultured on PLGA were positive for immunofluorescent staining of osteocalcin and alizarin red S staining. The scaffold and osteogenic-like cells induced in vitro were implanted subcutaneously into SCID mice. In resected teratoma, hard tissues resembling bone were observed mixed with other tissues on the scaffold. The sum of these findings suggests that PLGA does not disturb the osteogenesis of iPS cells.

show abstract

Natural and Synthetic Materials for Self‐Renewal, Long‐Term Maintenance, and Differentiation of Induced Pluripotent Stem Cells

Dzhoyashvili

Shen

Rochev

2015

Adv Healthcare Materials

View full text Add to dashboard Cite

Induced pluripotent stem cells (iPSCs) have attracted considerable attention from the public, clinicians, and scientists since their discovery in 2006, and raised huge expectations for regenerative medicine. One of the distinctive features of iPSCs is their propensity to differentiate into the cells of three germ lines in vitro and in vivo. The human iPSCs can be used to study the mechanisms underlying a disease and to monitor the disease progression, for testing drugs in vitro, and for cell therapy, avoiding many ethical and immunologic concerns. This technology offers the potential to take an individual approach to each patient and allows a more accurate diagnosis and specific treatment. However, there are several obstacles that impede the use of iPSCs. The derivation of fully reprogrammed iPSCs is expensive, time-consuming, and demands meticulous attention to many details. The use of biomaterials could increase the efficacy and safety while decreasing the cost of tissue engineering. The choice of a substrate utilized for iPSC culture is also important because cell-substrate contacts influence cellular behavior such as self-renewal, expansion, and differentiation. This Progress Report aims to summarize the advantages and drawbacks of natural and synthetic biomaterials, and to evaluate their role for maintenance and differentiation of iPSCs.

show abstract

A simple method for deriving functional MSCs and applied for osteogenesis in 3D scaffolds

Cited by 112 publications

References 33 publications

Craniofacial Bone Regeneration using iPS Cell-Derived Neural Crest Like Cells

Craniofacial Bone Regeneration using iPS Cell-Derived Neural Crest Like Cells

Differentiation behavior of iPS cells cultured on PLGA with osteoinduction medium

Natural and Synthetic Materials for Self‐Renewal, Long‐Term Maintenance, and Differentiation of Induced Pluripotent Stem Cells

Contact Info

Product

Resources

About